Visualization and tracking of live type a spermatogonia using a fluorescence-conjugated antibody in Salmo species

Ichida, Kensuke; Matsushita, Yoshiyuki; Amano, Yuichi; Miwa, Misako; Nagasawa, Kazue; Hayashi, Makoto; Mizutani, Hanaka; Takahashi, Momo; Boonanuntanasarn, Surintorn; Yoshizaki, Goro

doi:10.1016/j.aquaculture.2020.736096

Cited by 5 publications

(7 citation statements)

References 40 publications

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“…In non-transgenic fish, GFP - nos1 3′ UTR mRNAs were injected into 1 to 4-cell stage embryos to label PGCs, which resulted in reaching 100% purity after FACS [ 17 ]. Fluorophore-conjugated antibodies that were raised against fish ASG surface antigens led to 70.7–80.9% ASG purity after FACS in brown trout ( Salmo trutta ) [ 86 ], Pacific bluefin tuna [ 45 ], and rainbow trout [ 20 ]. Without fluorescence labeling, the enrichment of ASG was achieved by only the parameters such as forward scatter (FS; for cell size) and side scatter (SS; for granularity).…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…Thus, employing the antibodies against specific surface proteins of GSCs seems one of the appropriate options to obtain highly enriched GSCs for aquaculture applications. However, the purities of fish GSCs after FACS with antibodies were 70.7–80.9% [ 20 , 45 , 86 ], which are lower than the purities (75.6–94.9%) achieved in FS and SS methods [ 19 , 87 ] and the purities (higher than 90%) enriched by FACS with antibodies in mammalian studies [ 91 , 92 , 93 ]. In addition, rainbow trout GSC antibodies (No.…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…By combining them, the limitation of each method can be mitigated, which would lead to higher purities than those of enrichment with a single method [ 70 ]. The improved purity of GSCs after enrichment resulted in an increase of colonization efficiencies (up to a 17.5-fold increase) compared to those of unsorted cells [ 19 , 20 , 62 , 70 , 73 , 86 ]. Improved colonization rates by enriching donor cells will contribute to efficient surrogate offspring production, reducing efforts for screening works.…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…During in vitro culture, the characteristics of spermatogonia were maintained, demonstrated by transplantation assay [ 147 ]. Considering that germ cells from other salmonids could be nursed in the microenvironmental conditions supplied by rainbow trout recipients after transplantation [ 2 , 19 , 62 , 86 , 150 ], this culture system is probably able to apply to other salmonids or extend to other fish to propagate donor germ cells. Further studies on enhancing proliferation, maintaining GSC characteristics, and prolonging the culture period without causing GSC differentiation would enable a sufficient supply of donor cells for germ cell transplantation.…”

Section: Obstacles and Potential Solutions Of Germ Cell Transplantati...mentioning

confidence: 99%

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Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Ryu

Wong

2022

Animals

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Germ cell transplantation technology enables surrogate offspring production in fish. This technology has been expected to mitigate reproductive barriers, such as long generation time, limited fecundity, and complex broodstock management, enhancing seed production and productivity in aquaculture. Many studies of germ cell transplantation in various fish species have been reported over a few decades. So far, surrogate offspring production has been achieved in many commercial species. In addition, the knowledge of fish germ cell biology and the related technologies that can enhance transplantation efficiency and productivity has been developed. Nevertheless, the commercial application of this technology still seems to lag behind, indicating that the established models are neither beneficial nor cost-effective enough to attract potential commercial users of this technology. Furthermore, there are existing bottlenecks in practical aspects such as impractical shortening of generation time, shortage of donor cells with limited resources, low efficiency, and unsuccessful surrogate offspring production in some fish species. These obstacles need to be overcome through further technology developments. Thus, we thoroughly reviewed the studies on fish germ cell transplantation reported to date, focusing on the practicality, and proposed potential solutions and future perspectives.

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Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Obstacles and Potential Solutions Of Germ Cell Transplantati...mentioning

confidence: 99%

See 2 more Smart Citations

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Ryu

Wong

2022

Animals

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show abstract

“…Both of these methods can be combined with differential plating for further elimination of somatic cells based on their adhesive properties to the culture plate ( Lacerda et al, 2010 ; Shikina et al, 2008 ). Alternative methods to enrich gonial cells include cell sorting based on their light-scattering characteristics ( Ichida et al, 2017 ; Kise et al, 2012 ), or the use of gonial cell specific antibodies with flow cytometry, FACS or magnetic-activated cell sorting (MACS) ( Hayashi et al, 2019 ; Ichida et al, 2020 , Ichida et al, 2019 , Ichida et al, 2019 ). However, it should be noted that these processes may damage gonial cells or alter their physiological features ( Xie et al, 2020 ).…”

Section: Surrogate Broodstock Technologymentioning

confidence: 99%

Surrogate broodstock to enhance biotechnology research and applications in aquaculture

Jin

Robledo

Hickey

et al. 2021

Biotechnology Advances

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Aquaculture is playing an increasingly important role in meeting global demands for seafood, particularly in low and middle income countries. Genetic improvement of aquaculture species has major untapped potential to help achieve this, with selective breeding and genome editing offering exciting avenues to expedite this process. However, limitations to these breeding and editing approaches include long generation intervals of many fish species, alongside both technical and regulatory barriers to the application of genome editing in commercial production. Surrogate broodstock technology facilitates the production of donor-derived gametes in surrogate parents, and comprises transplantation of germ cells of donors into sterilised recipients. There are many successful examples of intra- and inter-species germ cell transfer and production of viable offspring in finfish, and this leads to new opportunities to address the aforementioned limitations. Firstly, surrogate broodstock technology raises the opportunity to improve genome editing via the use of cultured germ cells, to reduce mosaicism and potentially enable in vivo CRISPR screens in the progeny of surrogate parents. Secondly, the technology has pertinent applications in preservation of aquatic genetic resources, and in facilitating breeding of high-value species which are otherwise difficult to rear in captivity. Thirdly, it holds potential to drastically reduce the effective generation interval in aquaculture breeding programmes, expediting the rate of genetic gain. Finally, it provides new opportunities for dissemination of tailored, potentially genome edited, production animals of high genetic merit for farming. This review focuses on the state-of-the-art of surrogate broodstock technology, and discusses the next steps for its applications in research and production. The integration and synergy of genomics, genome editing, and reproductive technologies have exceptional potential to expedite genetic gain in aquaculture species in the coming decades.

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Development of spermatogonial cell transplantation in barramundi (Asian seabass: Lates calcarifer)

Ichida,

Tani,

Sreebun

et al. 2024

Aquaculture

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Visualization and tracking of live type a spermatogonia using a fluorescence-conjugated antibody in Salmo species

Cited by 5 publications

References 40 publications

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Surrogate broodstock to enhance biotechnology research and applications in aquaculture

Development of spermatogonial cell transplantation in barramundi (Asian seabass: Lates calcarifer)

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