Visualisation of human plasma fibrinogen adsorbed on titanium implant surfaces with different roughness

Cacciafesta, Paola; Hallam, K; Watkinson, A C; Allen, GC; Miles, Mervyn J; Jandt, Klaus D.

doi:10.1016/s0039-6028(01)01303-6

Cited by 83 publications

(66 citation statements)

References 47 publications

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“…AFM imaging was performed on a Digital Instruments NanoScope III. The topographic image on the AFM was obtained by the tapping mode using the changes in the cantilever oscillation amplitude, but in the case of the phase image, the phase lag of the cantilever motion relative to the driving oscillator was also registered and used to generate images [17,[36][37][38][39]. ESEM signals were collected in low vacuum mode, with an off axis gaseous secondary electron detector.…”

Section: Dense Discsmentioning

confidence: 99%

Effect of Protein Adsorption onto the Dissolution of Silicon-Substituted Hydroxyapatite

Botelho¹,

Brooks²,

Kanitakahara³

et al. 2011

JEAS

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Several authors have shown the beneficial role of silicon incorporate into the hydroxyapatite lattice, although, the mechanism behind the enhanced bioactivity of this Si-hydroxyapatite (Si-HA) is poorly understood. The incorporation of Si into the HA lattice alters the surface charge of HA, leading to more negative values. Due to the importance of the surface properties on the interaction between biomaterials, physiological fluids, and the host tissue, it is important to further characterize the surface of Si-HA by determining its surface energy and wettability. Our results showed that the incorporation of Si increased the hydrophilicity of HA, leading to a higher interfacial tension. Another important property for osteointegration is the formation of an apatite layer. The dissolution of Si-HA in the presence of serum-free simulated body fluid (SBF) started at early time points and using atomic force microscopy (AFM) it was possible to observe the dissolution at the grain boundaries and grains. As the dissolutionprecipitation process is much more complex in vivo, we tried to mimic the initial stages of the in vivo reaction by incubating the Si-HA in serum-SBF. It was shown that the dissolution kinetics in serum-SBF was slower when compared to the dissolution in serum free-SBF. At the same time point, no significant dissolution features were observed or apatite layer was visualized. The phase imaging AFM indicated the presence of a layer on top of these materials that could be a proteinaceous layer, as XPS analysis detected an increase on the concentration of nitrogen on the surface of the samples incubated in the presence of proteins.

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Section: Dense Discsmentioning

confidence: 99%

Effect of Protein Adsorption onto the Dissolution of Silicon-Substituted Hydroxyapatite

Botelho¹,

Brooks²,

Kanitakahara³

et al. 2011

JEAS

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“…Human plasma fibrinogen (HPF) is the relevant protein, which adsorbs on biomaterial surfaces. HPF partakes in blood coagulation, facilitates adhesion and aggregation of platelets (Cacciafesta et al, 2001;. The structure and composition of the adsorbed protein layer determine the type and extent of the subsequent biological reactions, such as activation of coagulation and immune response and osseointegration (Nygren et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…When measuring in liquid environment the potential amount of usable devices rapidly decreases and none of the listed devices are alone able to describe, in detail, the adsorption process of biomolecules. (Agnihotri & Siedlecki, 2004;Cacciafesta et al, 2001;Cai et al, 2006;2005;Höök et al, 2002b;Jackson et al, 2000;Jandt, 2001;Roach et al, 2005;Roach, Shirtcliffe, Farrar & Perry, 2006;Soman et al, 2008;Sonesson et al, 2007;Swann et al, 2004;Toscano & Santore, 2006;Van De Keere et al, 2008;Vanderah et al, 2004;Wang et al, 2003;Wertz & Santore, 1999;2001;2002;Xu & Siedlecki, 2007) Adsorption of HPF molecules at the rough bulk titanium and modified titanium surfaces increases the scattering and decreases the coherence of the probing laser light beam. These changes in coherence as well as in signal magnitude can be detected with diffractive optical element (DOE) based sensor.…”

Section: Introductionmentioning

confidence: 99%

Optical Detection of Protein Adsorption on Doped Titanium Surface

Silvennoinen¹,

Penttinen²,

Silvennoinen³

et al. 2011

Biomaterials Science and Engineering

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“…HPF is one of the most relevant proteins that are adsorbed on biomaterial surfaces. It takes part in blood coagulation and facilitates adhesion and aggregation of platelets [8,17]. The adsorption of HPF on titanium samples was studied using Atomic Force Microscopy (AFM), which tends to be an appropriate technique for surfaces analysis, even in the presence of biomolecules [8,[18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Fibrinogen and cellular adherability on differently treated titanium as implants

et al. 2011

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Ê Ú ¾ ÂÙÒ ¾¼½½ ÔØ ½ Ë ÔØ Ñ Ö ¾¼½½ ×ØÖ ØAdsorption of human plasma fibrinogen, osteoblasts, and fibroblasts on differently treated titanium samples as implants were examined in this study. Titanium samples were mechanically polished, chemically etched (with and without surface material loss), and grinded. The main goal of this study is to find the best surface treatment of titanium for its possible use as implants. Atomic force microscopy was used to evaluate the adsorption of human plasma fibrinogen onto the titanium samples. Cell counting was used to determine the adherability of osteoblasts and fibroblasts on the titanium samples. Our preliminary results show that the etched titanium surface with surface material loss is the best surface treatment used in our experiments.

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Visualisation of human plasma fibrinogen adsorbed on titanium implant surfaces with different roughness

Cited by 83 publications

References 47 publications

Effect of Protein Adsorption onto the Dissolution of Silicon-Substituted Hydroxyapatite

Effect of Protein Adsorption onto the Dissolution of Silicon-Substituted Hydroxyapatite

Optical Detection of Protein Adsorption on Doped Titanium Surface

Fibrinogen and cellular adherability on differently treated titanium as implants

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