Visual detection of cyclobutane pyrimidine dimer DNA damage lesions by Hg2+ and carbon dots

Singh, Seema; Singh, Manoj K.; Das, Prolay

doi:10.1016/j.aca.2018.02.029

Cited by 7 publications

(1 citation statement)

References 63 publications

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“…Given that CNPs potentially cause DNA damage [8,21], the present work explores the potential CNPs induced cell death signaling and combinatorial with PARP inhibitors to develop a novel conceptual link of carbon effect on cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells

Fan

Sun

Dukenbayev

et al. 2022

Preprint

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Background Quantum nanodots especially carbon nanoparticles (CNPs) have been widely studied in biomedicine in imaging, drug delivery, but anti-cancer mechanisms remain elusive. Methods Here we studied on a type of cell death induced by food (beet, soybean) derived CNPs in cancer cells and tested whether CNPs induced DNA damage and resistant to anticancer agent PARP inhibitor (PARPi) could be overcome by quantum calculation, SEM, TEM, AFM, FT-IR, soft agar assay, and cytotoxicity assay. Results At high doses, CNPs derived from beet lead to a pop-like apoptosis of cells. Quantum mechanical calculation confirmed CNPs binding with phosphate groups as well as DNA bases. At low doses, CNPs develop PARPi drug resistance through interactions between CNPs and PARPi. A synergistic drug effect was achieved with the combination of phosphatase inhibitor (PPi), PARPi and CNPs. This is corroborated by the fact that a sulfur modulated CNPs which exhibit super high phosphatase nanozyme activity abrogated the CNPs induced colony formation in anchorage-independent cancer cell growth. Conclusion Thus, our data suggest the CNPs intrinsic nanozyme activity of phosphatase may crosstalk with drug resistance, which can be reversed upon modulations.

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Section: Introductionmentioning

confidence: 99%

Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells

Fan

Sun

Dukenbayev

et al. 2022

Preprint

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PEITC Induces DNA Damage and Inhibits DNA Repair‐Associated Proteins in Human Retinoblastoma Cells In Vitro

Hsu,

Huang,

Hsia

et al. 2024

Environmental Toxicology

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Phenethyl isothiocyanate (PEITC), a natural product, exists in biological activities, including anticancer activity in many human cancer cells. No information shows that PEITC affects DNA damage in human retinoblastoma (RB) cells in vitro. In this study, the aim of experiments was to determine whether PEITC decreased total viable cell number or not by inducing protein expressions involved in DNA damage and repair in Y79 RB cells in vitro. Total cell viability was measured by PI exclusion assay, and PEITC reduced the total Y79 viable cell numbers in a dose‐dependent manner. DNA condensation and DNA impairment were conducted by DAPI staining and comet assays, respectively, in Y79 cells. The findings show that PEITC induced DNA condensation dose‐dependently based on the brighter fluorescence of cell nuclei stained by DAPI staining. PEITC‐induced DNA damage showed a more extended DNA migration smears than that of the control, which was performed by a comet assay. Western blotting was performed to measure the protein expressions involved in DNA damage and repair, which showed that PEITC at 2.5–10 μM increased NRF2, HO‐1, SOD (Mn), and catalase; however, it decreased SOD (Cu/Zn) except 10 μM PEITC treatment, and decreased glutathione, which were associated with oxidative stress. Furthermore, PEITC increased DNA‐PK, MDC1, H2A.XpSer139, ATMpSer1981, p53, p53pSer15, PARP, HSP70, and HSP90, but decreased TOPIIα, TOPIIβ, and MDM2pSer166 that were associated with DNA damage and repair mechanism in Y79 cells. The examination from confocal laser microscopy shows that PEITC increased H2A.XpSer139 and p53pSer15, and decreased glutathione and TOPIIα in Y79 cells. In conclusion, the cytotoxic effects of PEITC on reducing the number of viable cells may be due to the induction of DNA damage and the alteration of DNA repair proteins in Y79 cells in vitro.

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Solar-mediated oil-spill cleanup by a carbon dot-polyurethane sponge

Singh¹,

Jelinek

2020

Carbon

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Visual detection of cyclobutane pyrimidine dimer DNA damage lesions by Hg2+ and carbon dots

Cited by 7 publications

References 63 publications

Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells

Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells

PEITC Induces DNA Damage and Inhibits DNA Repair‐Associated Proteins in Human Retinoblastoma Cells In Vitro

Solar-mediated oil-spill cleanup by a carbon dot-polyurethane sponge

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