Viscolin, a new chalcone from Viscum coloratum, inhibits human neutrophil superoxide anion and elastase release via a cAMP-dependent pathway

Hwang, Tsong‐Long; Leu, Yann‐Lii; Kao, Shu-Hui; Tang, Ming-Chi; Chang, Han-Lin

doi:10.1016/j.freeradbiomed.2006.08.001

Cited by 106 publications

(89 citation statements)

References 34 publications

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“…•2 and the release of elastase in FMLP-activated human neutrophils (36,37). This contention is supported by a previous study, which reported that cAMP/protein kinase A increases the Ca 21 sequestering/ resequestering activity of ERCA by phosphorylation of the regulatory polypeptide, phospholamban (41).…”

Section: Discussionsupporting

confidence: 71%

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Suppression of superoxide anion and elastase release by C18 unsaturated fatty acids in human neutrophils

Hwang

Chang

et al. 2009

Journal of Lipid Research

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The structure-activity relationship of 18-carbon fatty acids (C 18 FAs) on human neutrophil functions and their underlying mechanism were investigated. C 18 unsaturated (U)FAs potently inhibited superoxide anion production, elastase release, and Ca 21 mobilization at concentrations of UFAs did not reduce extracellular Ba 21 entry in FMLP-and thapsigargin-activated neutrophils. In summary, the inhibition of neutrophil functions by C 18 UFAs is attributed to the blockade of Ca 21 mobilization through modulation of PMCA. We also suggest that both the free carboxy group and the number of double bonds of the C 18 UFA structure are critical to providing the potent anti-inflammatory properties in human neutrophils.-Hwang,

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Section: Discussionsupporting

confidence: 71%

“…•2 and the release of elastase, as well as to shorten the value of t 1/2 in FMLP-activated human neutrophils (36,37). To examine whether cAMP pathways are involved in the inhibitory effects of C 18 UFAs, cAMP concentrations and PDE activities were assayed.…”

Section: Ufas Inhibit Omentioning

confidence: 99%

Suppression of superoxide anion and elastase release by C18 unsaturated fatty acids in human neutrophils

Hwang

Chang

et al. 2009

Journal of Lipid Research

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“…Measurement of superoxide anion generation was based on the SOD-inhibitable reduction of ferricytochrome c (Hwang, Leu, Kao, Tang, & Chang, 2006). In brief, after supplementation with 0.5 mg/ml ferricytochrome c and 1 mM Ca 2+ , neutrophils (6 Â 10 5 /ml) were equilibrated at 37°C for 2 min and incubated with different concentrations of compounds or DMSO (as control) for 5 min.…”

Section: Measurement Of Superoxide Anion Generationmentioning

confidence: 99%

“…Degranulation of azurophilic granules was determined by measuring elastase release as described previously (Hwang et al, 2006). Experiments were performed using MeO-Suc-Ala-Ala-ProVal-p-nitroanilide as the elastase substrate.…”

Section: Measurement Of Elastase Releasementioning

confidence: 99%

New coumarins and anti-inflammatory constituents from Zanthoxylum avicennae

et al. 2012

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“…Degranulation of azurophilic granules was determined by measuring elastase release as described previously (Hwang et al, 2006). Experiments were performed using MeO-Suc-AlaAla-Pro-Val-p-nitroanilide as the elastase substrate.…”

Section: Measurement Of Elastase Releasementioning

confidence: 99%