Viscoelastic lithography for fabricating self-organizing soft micro-honeycomb structures with ultra-high aspect ratios

Jeong, Gi Seok; No, Da Yoon; Lee, Jaeseo; Yoon, Junghyo; Chung, Seok; Lee, Sang Hoon

doi:10.1038/ncomms11269

Cited by 40 publications

(17 citation statements)

References 31 publications

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“…Recent emerging technologies in biomaterials and tissue engineering enable the development of a complex structure for regeneration and precision medicine. 11,63 Despite such advancement, the fabrication of precisely organized and aligned cell-matrix structures mimicking real tissues has been challenging. Fabricating complex structure requires complex technologies, which makes the barrier higher.…”

Section: Discussionmentioning

confidence: 99%

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

et al. 2020

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“…Square, hexagonal, circular and triangular Some reports showed that hexagonal and circular shapes are better than others to form founded spheroid 6,27,36,37 ✓ ✓…”

Section: Conventional Lithography Fabricationmentioning

confidence: 99%

A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

Behroodi

Latifi

Bagheri

et al. 2020

Sci Rep

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The fabrication of a large-scale microfluidic mold with 3D microstructures for manufacturing of the conical microwell chip using a combined projection micro-stereolithography (PµSL) 3D printing/CNC micro-milling method for tumor spheroid formation is presented. The PµSL technique is known as the most promising method of manufacturing microfluidic chips due to the possibility of creating complex three-dimensional microstructures with high resolution in the range of several micrometers. The purpose of applying the proposed method is to investigate the influence of microwell depths on the formation of tumor spheroids. In the conventional methods, the construction of three-dimensional microstructures and multi-height chips is difficult, time-consuming, and is performed using a multi-step lithography process. Microwell depth is an essential parameter for microwell design since it directly affects the shear stress of the fluid flow and the diffusion of nutrients, respiratory gases, and growth factors. In this study, a chip was made with microwells of different depth varying from 100 to 500 µm. The mold of the microwell section is printed by the lab-made PµSL printer with 6 and 1 µm lateral and vertical resolutions. Other parts of the mold, such as the main chamber and micro-channels, were manufactured using the CNC micro-milling method. Finally, different parts of the master mold were assembled and used for PDMS casting. The proposed technique drastically simplifies the fabrication and rapid prototyping of large-scale microfluidic devices with high-resolution microstructures by combining 3D printing with the CNC micro-milling method.

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“…Several protocols have reported the fabrication of cell aggregates, referred to as spheroids [ 22 , 23 ], that are considered to be applicable to drug development, embryology, and regenerative medicine. Cell spheroids can be formed by various methods such as the hanging drop method [ 24 , 25 ], a non-adherent culture substratum [ 26 , 27 ], and rotating equipment [ 28 , 29 ]. However, these methods cannot produce large spheroids and it is also difficult to control the morphology of the cell aggregates.…”

Section: Introductionmentioning

confidence: 99%

Large three-dimensional cell constructs for tissue engineering

Sasaki

Abe

et al. 2021

Science and Technology of Advanced Materials

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Much research has been conducted on fabricating biomimetic biomaterials in vitro. Tissue engineering approaches are often conducted by combining cells, scaffolds, and growth factors. However, the degradation rate of scaffolds is difficult to control and the degradation byproducts occasionally limit tissue regeneration. To overcome these issues, we have developed a novel system using a thermo-responsive hydrogel that forms scaffold-free, three-dimensional (3D) cell constructs with arbitrary size and morphology. 3D cell constructs prepared using bone marrow-derived stromal stem cells (BMSCs) exhibited self-organizing ability and formed bone-like tissue with endochondral ossification. Endothelial cells were then introduced into the BMSC construct and a vessel-like structure was formed within the constructs. Additionally, the bone formation ability was promoted by endothelial cells and cell constructs could be freeze-dried to improve their clinical application. A pre-treatment with specific protein protectant allowed for the fabrication of novel bone substitutes composed only of cells. This 3D cell construct technology using thermo-responsive hydrogels was then applied to other cell species. Cell constructs composed of dental pulp stem cells were fabricated, and the resulting construct regenerated pulp-like tissue within a human pulpless tooth. In this review, we demonstrate the approaches for the in vitro fabrication of bone and dental pulp-like tissue using thermo-responsive hydrogels and their potential applications.

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Viscoelastic lithography for fabricating self-organizing soft micro-honeycomb structures with ultra-high aspect ratios

Cited by 40 publications

References 31 publications

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production

Large three-dimensional cell constructs for tissue engineering

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