Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

Jang, Yong-Jun; Jung, Da-Jung; Choi, Seung Cheol; Lim, Do Sun; Kim, Jong Hoon; Jeoung, Gi Seok; Kim, Jong‐Seong; Park, Yongdoo

doi:10.1039/d0ra01577f

Cited by 2 publications

(3 citation statements)

References 71 publications

(82 reference statements)

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“…In the following study, the accordion-like honeycomb (ALH) pattern was engaged with the LBL-based cardiac patch. The results show that cardiac cells were aligned with the pattern and highly matured in the LBL-layered cardiomyocyte and cardiac fibroblast group [9]. In another study, a cardiac patch was fabricated using collagen, i.e., a clinically approved material, and the electrospinning method to construct a substrate by controlling the deposition of polymer fibers using an electric field [99].…”

Section: Cardiac Patchesmentioning

confidence: 98%

“…On the other hand, combining and properties, and geometry, is essential to achieving mature cardiac tissue. On the other hand, combining and assembling heart cells, i.e., cardiomyocytes and cardiac fibroblasts in engineered cardiac tissues, is essential to form functional cardiac tissues that synchronize the contractility of cells in the engineered tissues [9]. Thus, micro-and macroscale understanding of cell-cell interactions is necessary, which is the purpose of various two-dimensional (2D) and three-dimensional (3D) approaches (see Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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Engineering Biomaterials to Guide Heart Cells for Matured Cardiac Tissue

2020

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The extracellular matrix (ECM) is needed to maintain the structural integrity of tissues and to mediate cellular dynamics. Its main components are fibrous proteins and glycosaminoglycans, which provide a suitable environment for biological functions. Thus, biomaterials with ECM-like properties have been extensively developed by modulating their key components and properties. In the field of cardiac tissue engineering, the use of biomaterials offers several advantages in that biophysical and biochemical cues can be designed to mediate cardiac cells, which is critical for maturation and regeneration. This suggests that understanding biomaterials and their use in vivo and in vitro is beneficial in terms of advancing cardiac engineering. The current review provides an overview of both natural and synthetic biomaterials and their use in cardiac engineering. In addition, we focus on different strategies to recapitulate the cardiac tissue in 2D and 3D approaches, which is an important step for the maturation of cardiac tissues toward regeneration of the adult heart.

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Section: Cardiac Patchesmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Engineering Biomaterials to Guide Heart Cells for Matured Cardiac Tissue

2020

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“…[387] Among all device architectures and morphologies, CPs fibers-and -pillars based platforms [388] demonstrated to increase the coupling efficiency matching the highly anisotropic CMs assembly [389] and avoiding devices repositioning and so aversive reactions such as inflammation, rejection or break. [135] Considering cardiac tissue engineering, the main challenge relates to the re-establishment of the complexity of the heart tissue following injuries through the development of cardiaclike platforms [390] that are capable of assisting the synchronous contractions [391,392] elicited by electrical signals as well as facilitating the oxygen exchange between the cells and blood. [393] In this context EFs stimulation through complex structures, such as hydrogel-based microchamber with PEDOT electrodes platforms, has proved to recreate the environmental signals on CMs monolayers allowing to maintain cardiac phenotype and contractile function in vitro while enhancing cellular elongation, cardiac maturation, beating performances and gap junction organization.…”

Section: From Blood Vessels Till the Blood Brain Barrier: A Long Route Passing Through The Heartmentioning

confidence: 99%

Conductive Polymer‐Based Bioelectronic Platforms toward Sustainable and Biointegrated Devices: A Journey from Skin to Brain across Human Body Interfaces

Bettucci

Matrone

Santoro

2021

Adv Materials Technologies

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the human's body toward medical applications. [1] Coupling electronics with human tissues allows sensing, stimulating and possibly regulating biological functions. On these premises, a key paradigm of bioelectronics is to preserve the functionality of the biological environment upon coupling, in order to "observe biology through non-perturbing lenses". However, aiming to a seamless interface, the properties of common electronic components, based on metals or inorganic semiconductors, have major limitations to comply with the coupling requirements for cells, organs, and tissues. [2] In fact, inorganic materials might display mechanical rigidity (high Young's modulus ≈100 GPa), [3,4] surface degradation phenomena (oxidation) [5] and surface structure which increases interface capacitance. [6,7] Organic materials have so emerged combining mechanical flexibility, compatibility with large area and different surface functionalization processes, while keeping high electrical conductivity and reproducibility. [8,9] Particularly, these materials intrinsically display key properties such as tunable surface roughness, [10] surface chemical reactivity 11 , and Young's moduli ranging from 20 kPa to 3 GPa, [11,12] approaching the modulus of living tissue (10 kPa [13] ). However, the diversity of biological landscapes offered by the different human tissues, in terms of mechanical flexibility, interface functionalization, accessibility and biological activities defines sets of requirements so stringent that commonly for each tissue/organ coupling ad hoc devices and platforms have been developed. Hence, examining the three major human's body interfaces targeted by bioelectronics applications (skin, heart, and brain), this review focuses on the strategies that can be adopted by employing organic materials such as surface patterning, novel material synthesis and bio-functionalization in order to enhance tissue/organ-specific coupling performances. These aspects are investigated highlighting current and future main challenges and providing perspectives on the development of the next generation organic bioelectronics devices, thus envisioning systems that are: 1) able to adapt their interface in shape and size to comply with different curvilinear and hierarchical biological architectures and 2) combine sensing and stimulation to dynamically control biological functions for biomedical applications.

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Multidimensional assembly using layer-by-layer deposition for synchronized cardiac macro tissues

Abstract: We fabricated a cardiac macro tissue with synchronized beating by layer-by-layer deposition and evaluated the effect of drug candidates.

Cited by 2 publications

References 71 publications

Engineering Biomaterials to Guide Heart Cells for Matured Cardiac Tissue

Engineering Biomaterials to Guide Heart Cells for Matured Cardiac Tissue

Conductive Polymer‐Based Bioelectronic Platforms toward Sustainable and Biointegrated Devices: A Journey from Skin to Brain across Human Body Interfaces

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