Natural environment has undergone rapid transformations, primarily attributed to human actions that threaten ecosystems' balance. Understanding the relationship between humans and nature is essential to generate effective strategies for sustainably using resources. In this study, we analyzed the environmental perception and knowledge about the biology of sea turtles through 120 informal interviews with visitors at beaches on the north-central coast of Rio de Janeiro state. Residents noticed garbage more often than tourists, as did women and younger individuals. The perception of initiatives necessary to increase the preservation of the beaches was higher for people with higher education. In general, there were no differences in biological knowledge about sea turtles between residents, tourists and people who work or study at the cities. The results point to the need for oceanic education strategies that can help raise environmental awareness through long-term conservation programs.