During bovine enterovirus infection of Ehrlich ascites tumor cells, large amounts of doublestranded RNA accumulate. Addition of this doublestranded RNA to uninfected cells leads to rapid cell death. This is not a result of infectious virus production. Neither single-stranded RNA nor heat-denatured double-stranded RNA has this effect. Similar experiments with synthetic double-stranded polymers, poly(I) *poly(C) and poly(A). poly(U), show that they are only slightly toxic at the concentrations used. The effect of the double-stranded RNA is nonspecific for cells of different origins. The implications of this finding in relation to the cytopathic effects of picornavirus and to cancer chemotherapy are discussed.During the replication cycle of picornaviruses, doublestranded RNA is formed as an intermediate (1). In polio-and mengovirus infected cells, this double-stranded RNA is detectable 3-5 hr after infection (2, 3). In bovine enterovirus (BEV) replication, double-stranded RNA is detectable 2 hr after infection (manuscript in preparation). Unlike mengovirus-and poliovirus-infected cells, in which only 1-5% of the total amount of RNA is double-stranded, in BEV-infected cells, double-stranded RNA accumulates throughout the infection cycle; 7 hr after infection, just before cell death, it is the major species of viral RNA in the cells. In order to test whether there was any relationship between the accumulation of double-stranded RNA and the cytopathic effect, we have examined the effect of this RNA on various cells in culture. Our data suggest that viral double-stranded RNA is very toxic to such cells.
MATERIALS AND METHODS
Cells and virusMonolayer cultures of W138 were purchased from GIBCO (Grand Island, N.Y.) penicillin (100 units/ ml), streptomycin (100 gg/ml), and Fungizone (5 units/ml).Ehrlich ascites tumor and Sarcoma-1 were maintained as ascites tumors by intraperitoneal passage of about 108 cells at 8-day intervals in adult Swiss-Webster mice.Bovine enterovirus-1 (BEV) was grown as described (4). Titrations of the virus were performed on either L-cells or MDBK cells.