Virus production and hemoglobin synthesis in variant lines of dimethyl sulfoxide-treated Friend erythroleukemia cells

Tsuei, Deane; Haubenstock, Harriette; Revoltella, Roberto P.; Friend, Charlotte

doi:10.1128/jvi.31.1.178-183.1979

Cited by 14 publications

(4 citation statements)

References 34 publications

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“…Coupled with this increase is a change in the tropism of the helper virus, suggesting that DMSO treat-114 KERN AND AXELROD ment also causes the induction of an endogenous virus in MELC (9,31,32). Increased reverse transcriptase activity after DMSO treatment has also been reported in some MELC lines (22,32,42), as have increases in the amount of polysomal viral RNA (7,31,32,33). In addition, changes in the genome composition and the ratios of SFFV to helper virus have been observed in the virus complex that is released by DMSO-treated MELC.…”

mentioning

confidence: 76%

“…Cell lines resistant to the differentiation-inducing effects of DMSO that nevertheless show an increase in virus release upon exposure to DMSO have also been described. Therefore, it has been suggested that the augmentation of virus gene expression and the induction of differentiation are independent events (9, 22,38,42). It has also been suggested that this increase in virus synthesis may be related to the effects of DMSO on the cellular growth cycle of MELC (38).…”

mentioning

confidence: 99%

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Dimethyl sulfoxide affects the amount of extrachromosomal spleen focus-forming virus DNA in murine erythroleukemia cells

Kern¹,

Axelrod

1983

J Virol

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We used Southern blot hybridization to titrate and map restriction enzyme cleavage sites of a 6.3-kilobase-pair species of extrachromosomal viral DNA found in derivatives of the 745A line of murine erythroleukemia cells, which vary in their ability to be induced to differentiate by dimethyl sulfoxide (DMSO). Greater than an eightfold variation was observed in the amount of this DNA, with the largest amounts being found in cells that were resistant to the induction of differentiation by DMSO. This increase in the level of extrachromosomal viral DNA was found to be dependent upon the continued presence of DMSO in the culture medium. The increase was shown not to be due to an immediate stimulatory effect of this agent on the synthesis or maintenance of this DNA, since cell lines sensitive to the differentiation-inducing effects of DMSO were shown to undergo a transient reduction in the amount of extrachromosomal viral DNA after the addition of DMSO to the culture medium. In addition to the 6.3-kilobase-pair linear form found in the cytoplasm, in some preparations two hybridizing bands were observed that migrated in agarose gels in the position expected of covalently closed circular species of viral DNA. Restriction enzyme mapping of the cytoplasmic linear form indicated a close relationship of this DNA to two polycythemic strains of spleen focus-forming virus that have been molecularly cloned by other workers. No obvious change in the number or arrangement of chromosomal viral sequences could be detected after treating cells with DMSO. Thus, the exposure of murine erythroleukemia cells to DMSO caused an obvious change in the amount of extrachromosomal spleen focus-forming virus DNA but no obvious change in the integration of the provirus.

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mentioning

confidence: 76%

mentioning

confidence: 99%

Dimethyl sulfoxide affects the amount of extrachromosomal spleen focus-forming virus DNA in murine erythroleukemia cells

Kern¹,

Axelrod

1983

J Virol

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“…Several studies have reported that DMSO affects the life cycles of viruses. For example, the replication or virus of fowl plague virus ( Scholtissek and Muller, 1988 ), human immunodeficiency virus 1 ( Taddeo et al, 2000 ), and duck hepatitis B virus ( Tsuei et al, 1979 ) is increased by DMSO. In addition, DMSO induces reactivation of murine cytomegalovirus in latently infected mouse spleens ( Blackett et al, 1987 ).…”

Section: Discussionmentioning

confidence: 99%

Dimethyl Sulfoxide Enhances Kaposi’s Sarcoma-Associated Herpesvirus Production During Lytic Replication

et al. 2021

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Kaposi’s sarcoma-associated herpesvirus (KSHV) is an etiologic agent of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman disease. In studies of KSHV, efficient virus production and isolation are essential. Reactivation of KSHV can be initiated by treating latently infected cells with chemicals, such as 12-O-tetradecanoyl-phorbol-13-acetate and sodium butyrate. These chemicals have been used as tools to induce lytic replication and viral production in KSHV-producing cell lines. Dimethyl sulfoxide (DMSO) is an organosulfur compound that is frequently used as an aprotic solvent similar to water. In experiments exploring signaling pathways in KSHV-infected cells, DMSO treatment alone as a vehicle affected the lytic gene expression of KSHV. However, to the best of our knowledge, the effects of DMSO on KSHV-producing cells have not yet been reported. Therefore, in this study, we investigated whether DMSO could be used as a reagent to enhance viral production during lytic replication in KSHV-producing cells and assessed the underlying mechanisms. The effects of DMSO on KSHV production were analyzed in iSLK BAC16 cells, which have been widely used for recombinant KSHV production. We found that the production of KSHV virions was significantly increased by treatment with DMSO during the induction of lytic replication. Mechanistically, lytic genes of KSHV were enhanced by DMSO treatment, which was correlated with virion production. Additionally, DMSO induced the phosphorylation of JNK during lytic replication, and inhibition of JNK abolished the effects of DMSO on lytic replication and virion production. Our findings showed that additional treatment with DMSO during the induction of lytic replication significantly improved the yield of KSHV production.

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“…The culture fluids were also tested for reverse transcriptase (RT) activity as described by Tsuei et al (1979), and for FLV antigens by com etition p30 (4 ng), and highly specific goat anti-FLV gp71 (1:300) and anti-FLV p30 (1 :200) sera as previously described (Bilello et al, 1974). For electron microscopy, the cells were fixed in glutaraldehyde (2% in 0.1 M cacodylate buffer, pH 7.2) and processed by standard procedures (Gudat and Willinger, 1973).…”

Section: Other Assaysmentioning

confidence: 99%

Lack of correlation between in vivo and in vitro assays for the detection of virus released from clones of friend erythroleukemia cells

Bertolini

Revoltella

Bendinelli

et al. 1981

Intl Journal of Cancer

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The biological properties of the virus synthesized by 18 clones of a line of mouse bone-marrow hematopoietic cells transformed in vitro by the polycythemic strain of Friend leukemia virus (FLV-P) were compared. In vitro assays were performed to determine whether the virus released into the culture fluids was ecotropic or xenotropic, and in vivo assays were carried out to determine spleen focus formation and leukemogenicity in susceptible DBA/2J and BALB/c mice. A number of clones released virus which reproduced the entire range of effects typical of the FLY-P complex. However, in other clones, there appeared to be no correlation between the assays for leukemogenicity and the assays for either ecotropic virus, reverse transcriptase activity, or virus antigens. Xenotropic virus was not detected in any of the cultures. These results suggest that the FLV-P complex contains a heterogeneous population of viruses, but the possibility that the differences observed may be due to the inability of FLV-P to be expressed fully in some clones cannot be excluded.

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Virus production and hemoglobin synthesis in variant lines of dimethyl sulfoxide-treated Friend erythroleukemia cells

Cited by 14 publications

References 34 publications

Dimethyl sulfoxide affects the amount of extrachromosomal spleen focus-forming virus DNA in murine erythroleukemia cells

Dimethyl sulfoxide affects the amount of extrachromosomal spleen focus-forming virus DNA in murine erythroleukemia cells

Dimethyl Sulfoxide Enhances Kaposi’s Sarcoma-Associated Herpesvirus Production During Lytic Replication

Lack of correlation between in vivo and in vitro assays for the detection of virus released from clones of friend erythroleukemia cells

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