Virus-Like Particle Vaccine Confers Protection against a Lethal Newcastle Disease Virus Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals

Park, Jaekeun; Lee, Donghun; Yuk, Seong‐Su; Erdene-Ochir, Tseren-Ochir; Kwon, Jung‐Hoon; Noh, Jin-Yong; Kim, Byoung Yoon; Choi, Soo-Won; Kang, Sang-Moo; Lee, Joong‐Bok; Park, Seung‐Yong; Choi, In‐Soo; Song, Chang‐Seon

doi:10.1128/cvi.00636-13

Cited by 31 publications

(17 citation statements)

References 25 publications

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“…However, the vaccine could take a long time to initiate the protective immune system. Therefore, different strategies to either prevent the replication of NDV or to decrease its drastic impact on infected flocks are needed (Park et al, 2014). One of those potential strategies is to use medicinal plant extracts as antiviral agents against NDV either in cell culture or chicken embryo systems (Waihenya et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antiviral Activity of Different Medicinal Plants against Newcastle Disease Virus

Al-Hadid¹

2016

American Journal of Agricultural and Biological Sciences

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Newcastle Disease (ND) is a highly contagious viral disease that has a tremendous negative impact on the poultry industry worldwide. Plant extracts were prepared from five different medicinal plants and applied against Newcastle Disease Virus (NDV) to evaluate the antiviral replication in Specific Pathogen-Free (SPF) chicken embryos. Three dilutions from each plant extract were prepared and mixed with a fixed titer (10 4 EID 50 /mL) of virulent NDV strain Herts 33. The mix was inoculated into nine-day-old SPF chicken embryos that were monitored for five days. Real-time PCR and hemagglutination tests were conducted to evaluate the activity of NDV and its viral RNA titer. Some plant extracts showed a complete inhibition of NDV evidenced by the absence of embryo deaths, the absence of HA titer and viral RNA in the allantoic fluid. These plant extracts were from Moringa peregrina (leaves), Acacia cyanophylla (leaves), Eucalyptus camaldulensis (fruits) and Pistacia atlantica (leaves and stems). Other plant extracts showed partial inhibition of NDV, such as Ceratonia siliqua (leaves) and Eucalyptus camaldulensis (leaves). This experiment shows the potential of using medicinal plants as antiviral agents.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Antiviral Activity of Different Medicinal Plants against Newcastle Disease Virus

Al-Hadid¹

2016

American Journal of Agricultural and Biological Sciences

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“…HA (Genbank AY676035) and M1 (Genbank AY676047) genes of A/chicken/Korea/ES/2003 and the F gene (Genbank AV630423) of KR-005/00 were extracted from virus infected allantoic fluid and amplified by two-step reverse transcription-polymerase chain reaction (RT-PCR; RNeasy® Mini Kit, Omniscript™ RT Kit, Qiagen, Germany). For cDNA synthesis, Uni12 primer was used for HA and M1 genes and a 5′-ATGGGCTCCAAACTTTC-3′ primer for F gene, as previously described [ 25 , 26 ]. To enhance the interaction between the ectodomain of the NDV F and the AIV M1 proteins in particle assembly, we constructed a chimeric F/HA protein.…”

Section: Methodsmentioning

confidence: 99%

Chimeric Bivalent Virus-Like Particle Vaccine for H5N1 HPAI and ND Confers Protection against a Lethal Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals (DIVA)

et al. 2016

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Highly pathogenic avian influenza (HPAI) and Newcastle disease (ND) are considered as the most devastating poultry infections, owing to their worldwide distribution and economical threat. Vaccines have been widely used to control these diseases in the poultry industry in endemic countries. However, vaccination policy without differentiating infected animals from vaccinated animals (DIVA) makes the virus surveillance difficult. In this study, we developed a bivalent virus-like particle (VLP) vaccine that is composed of the hemagglutinin (HA) and matrix 1 (M1) proteins of the H5N1 HPAI virus (HPAIV) and a chimeric protein containing the ectodomain of the ND virus (NDV) fusion (F) protein fused with the cytoplasmic and transmembrane domains of the HPAIV HA protein. A single immunization of chickens with the chimeric VLP vaccine induced high levels of hemagglutination inhibition (HI) antibody titers against H5N1 HPAI virus and anti-NDV antibody detected in ELISA and protected chickens against subsequent lethal HPAIV and NDV infections. Furthermore, we could easily perform DIVA test using the commercial NP-cELISA tests against HPAIV and HI assay against NDV. These results strongly suggest that utilization of chimeric VLP vaccine in poultry species would be a promising strategy for the better control of HPAI and ND simultaneously.

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“…A few years ago, production of NDVLPs was achieved following the expression of M protein in combination with the NP and viral surface glycoproteins (F and HN) [ 157 ]. More so, coexpression of NDV F protein and avian influenza M1 protein effectively produced VLPs in baculovirus expression system [ 158 ]. Interestingly in the above studies, not only did the VLPs successfully incorporate the surface glycoproteins, but the proteins' structural conformation and biological functions such as fusogenicity, hemagglutination, and neuraminidase activities remained unaffected.…”

Section: Vaccination Strategiesmentioning

confidence: 99%

Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives

Bello

Yusoff

Ideris

et al. 2018

BioMed Research International

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Newcastle disease (ND) is one of the most devastating diseases that considerably cripple the global poultry industry. Because of its enormous socioeconomic importance and potential to rapidly spread to naïve birds in the vicinity, ND is included among the list of avian diseases that must be notified to the OIE immediately upon recognition. Currently, virus isolation followed by its serological or molecular identification is regarded as the gold standard method of ND diagnosis. However, this method is generally slow and requires specialised laboratory with biosafety containment facilities, making it of little relevance under epidemic situations where rapid diagnosis is seriously needed. Thus, molecular based diagnostics have evolved to overcome some of these difficulties, but the extensive genetic diversity of the virus ensures that isolates with mutations at the primer/probe binding sites escape detection using these assays. This diagnostic dilemma leads to the emergence of cutting-edge technologies such as next-generation sequencing (NGS) which have so far proven to be promising in terms of rapid, sensitive, and accurate recognition of virulent Newcastle disease virus (NDV) isolates even in mixed infections. As regards disease control strategies, conventional ND vaccines have stood the test of time by demonstrating track record of protective efficacy in the last 60 years. However, these vaccines are unable to block the replication and shedding of most of the currently circulating phylogenetically divergent virulent NDV isolates. Hence, rationally designed vaccines targeting the prevailing genotypes, the so-called genotype-matched vaccines, are highly needed to overcome these vaccination related challenges. Among the recently evolving technologies for the development of genotype-matched vaccines, reverse genetics-based live attenuated vaccines obviously appeared to be the most promising candidates. In this review, a comprehensive description of the current and emerging trends in the detection, identification, and control of ND in poultry are provided. The strengths and weaknesses of each of those techniques are also emphasised.

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Virus-Like Particle Vaccine Confers Protection against a Lethal Newcastle Disease Virus Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals

Cited by 31 publications

References 25 publications

Evaluation of Antiviral Activity of Different Medicinal Plants against Newcastle Disease Virus

Evaluation of Antiviral Activity of Different Medicinal Plants against Newcastle Disease Virus

Chimeric Bivalent Virus-Like Particle Vaccine for H5N1 HPAI and ND Confers Protection against a Lethal Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals (DIVA)

Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives

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