Virus-induced gene silencing (VIGS) in Cysticapnos vesicaria, a zygomorphic-flowered Papaveraceae (Ranunculales, basal eudicots)

Hidalgo, Oriane; Bartholmes, Conny; Gleissberg, Stefan

doi:10.1093/aob/mcs008

Cited by 24 publications

(19 citation statements)

References 54 publications

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“…Similar evolutionary changes are reported in comparisons of whole transcriptional programmes in floral organs [20,85,87,88]. Much progress has been made in our understanding of floral developmental genetics in Ranunculales [78,[89][90][91][92][93][94], but the basal eudicot grade has not been representatively studied to date. The available data suggest that genetic programmes for floral organ identity are often more broadly deployed in the flowers of non-Pentapetalae angiosperms than in the flowers of Pentapetalae.…”

Section: Genetic Origin Of the Pentapetalae Flowersupporting

confidence: 58%

Evolution of floral diversity: genomics, genes andgamma

Chanderbali

Berger

Howarth

et al. 2017

Phil. Trans. R. Soc. B

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One contribution of 17 to a theme issue 'Evo-devo in the genomics era, and the origins of morphological diversity'. A salient feature of flowering plant diversification is the emergence of a novel suite of floral features coinciding with the origin of the most species-rich lineage, Pentapetalae. Advances in phylogenetics, developmental genetics and genomics, including new analyses presented here, are helping to reconstruct the specific evolutionary steps involved in the evolution of this clade. The enormous floral diversity among Pentapetalae appears to be built on a highly conserved ground plan of five-parted (pentamerous) flowers with whorled phyllotaxis. By contrast, lability in the number and arrangement of component parts of the flower characterize the early-diverging eudicot lineages subtending Pentapetalae. The diversification of Pentapetalae also coincides closely with ancient hexaploidy, referred to as the gamma whole-genome triplication, for which the phylogenetic timing, mechanistic details and molecular evolutionary consequences are as yet not fully resolved. Transcription factors regulating floral development often persist in duplicate or triplicate in gamma-derived genomes, and both individual genes and whole transcriptional programmes exhibit a shift from broadly overlapping to tightly defined expression domains in Pentapetalae flowers. Investigations of these changes associated with the origin of Pentapetalae can lead to a more comprehensive understanding of what is arguably one of the most important evolutionary diversification events within terrestrial plants.This article is part of the themed issue 'Evo-devo in the genomics era, and the origins of morphological diversity'.

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Section: Genetic Origin Of the Pentapetalae Flowersupporting

confidence: 58%

Evolution of floral diversity: genomics, genes andgamma

Chanderbali

Berger

Howarth

et al. 2017

Phil. Trans. R. Soc. B

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“…() for E. californica and in Hidalgo et al . () for C. vesicaria . Signals were recorded using a Leitz Laborlux S microscope equipped with a Leica DFC420 C digital camera (Wetzlar, Germany).…”

Section: Methodsmentioning

confidence: 97%

“…Ranunculales include several model species that are susceptible to functional genetics by virus-induced gene silencing (VIGS). Among these are the Papaveraceae representatives Papaver somniferum (Hileman et al, 2005), Eschscholzia californica (Wege et al, 2007) and Cysticapnos vesicaria (Hidalgo et al, 2012), and, in Ranunculaceae, Aquilegia coerulea (Gould & Kramer, 2007), Thalictrum ssp. (Di Stilio et al, 2010) and Nigella damascena (Gonc ßalves et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary diversification of CYC/TB1‐like TCP homologs and their recruitment for the control of branching and floral morphology in Papaveraceae (basal eudicots)

et al. 2018

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Angiosperms possess enormous morphological variation in plant architectures and floral forms. Previous studies in Pentapetalae and monocots have demonstrated the involvement of TCP domain CYCLOIDEA/TEOSINTE BRANCHED1-like (CYC/TB1) genes in the control of floral symmetry and shoot branching. However, how TCP/CYC-like (CYL) genes originated, evolved and functionally diversified remain unclear. We conducted a comparative functional study in Ranunculales, the sister lineage to all other eudicots, between Eschscholzia californica and Cysticapnos vesicaria, two species of Papaveraceae with actinomorphic and zygomorphic flowers, respectively. Phylogenetic analysis indicates that CYL genes in Papaveraceae form two paralogous lineages, PapaCYL1 and PapaCYL2. Papaveraceae CYL genes show highly diversified expression patterns as well as functions. Enhanced branching by silencing of EscaCYL1 suggests that the role of CYC/TB1-like genes in branching control is conserved in Papaveraceae. In contrast to the arrest of stamen development in Pentapetalae, PapaCYL genes promote stamen initiation and growth. In addition, we demonstrate that CyveCYLs are involved in perianth development, specifying sepal and petal identity in Cysticapnos by regulating the B-class floral organ identity genes. Our data also suggest the involvement of CyveCYL genes in the regulation of flower symmetry in Cysticapnos. Our work provides evidence of the importance of TCP/CYC-like genes in the promotion of morphological diversity across angiosperms.

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“…They have found that silencing of LAP profoundly increased the larval mass of Manduca sexta which fed on silenced plants demonstrating its involvement in defensive function against herbivores [4]. Agrobacterium tumefaciens mediated transfer of CvPDS gene (Phytoene desaturase) and CvFLOgene (Floricaula) into Cysticapnos vesicaria (Papaveraceae, basal eudicots) through Tobacco rattle virus (TRV)-based vectors have resulted in strong photobleaching of green parts and reduction of endogenous CvPDS transcript levels and affected floral phyllotaxis, symmetry and floral organ identities respectively [5]. Glutathione S-transferase (GST) was known to be up-regulated during the infection of fungal pathogen O. neolycopersici in resistant tomato plants (Solanum habrochiates G1.1560) that resulted in the accumulation of H 2 O 2 and Hypersensitive reaction (HR) and retarded the growth of the pathogen.…”

Section: Recent Advances On Vigs In Plant Functional Studymentioning

confidence: 99%