Recent Advances on Virus Induced Gene Silencing (VIGS): Plant Functional Genomics

Gupta, Bhaskar

doi:10.4172/2329-9029.1000e116

Cited by 11 publications

(8 citation statements)

References 15 publications

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“…Replication in the dsRNA intermediate would be processed in a way that siRNA present in the damaged cells would correspond to parts of the viral vector genome and also including any non-viral insert. Thus, when insertion is made in the host cell, the RNase complex is targeted by siRNA to the corresponding host mRNA and symptoms reveal the loss of function of the encoded protein in the infected plant [215]. In recent years, VIGS has been applied successfully in plant reverse genomics.…”

Section: Virus-induced Gene Silencing (Vigs)mentioning

confidence: 99%

DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing

Nadeem

Nawaz

Shahid

et al. 2017

Biotechnology & Biotechnological Equipment

557

327

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With the development of molecular marker technology in the 1980s, the fate of plant breeding has changed. Different types of molecular markers have been developed and advancement in sequencing technologies has geared crop improvement. To explore the knowledge about molecular markers, several reviews have been published in the last three decades; however, all these reviews were meant for researchers with advanced knowledge of molecular genetics. This review is intended to be a synopsis of recent developments in molecular markers and their applications in plant breeding and is devoted to early researchers with a little or no knowledge of molecular markers. The progress made in molecular plant breeding, genetics, genomic selection and genome editing has contributed to a more comprehensive understanding of molecular markers and provided deeper insights into the diversity available for crops and greatly complemented breeding stratagems. Genotyping-by-sequencing and association mapping based on next-generation sequencing technologies have facilitated the identification of novel genetic markers for complex and unstructured populations. Altogether, the history, the types of markers, their application in plant sciences and breeding, and some recent advancements in genomic selection and genome editing are discussed.

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Section: Virus-induced Gene Silencing (Vigs)mentioning

confidence: 99%

DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing

Nadeem

Nawaz

Shahid

et al. 2017

Biotechnology & Biotechnological Equipment

557

327

View full text Add to dashboard Cite

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“…Silencing Complex) which screens for and destroys RNAs complementary to the siRNA [25,[53][54][55][56][57][58][59][60][61][62][63]. Interaction of viral siRNA with target RNA can lead to endonucleolytic cleavage and translational inhibition of cognate RNAs [1,22].…”

Section: Molecular Mechanism Of Vigsmentioning

confidence: 99%

“…This facilitates high throughput silencing and multiple stress impositions. VIGS has also been used to silence genes during tissue culture and callus development [58] which can facilitate precise stress imposition and high throughput screening.…”

Section: Advances In Vigsmentioning

confidence: 99%

Applications of Virus Induced Gene Silencing (VIGS) in Plant Functional Genomics Studies

Bekele¹,

Tesfaye²,

Fikre³

2019

J Plant Biochem Physiol

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Virus induced gene silencing (VIGS) is an effective technology that exploits an antiviral defense mechanism in plants. It is a recently developed gene transcript suppression technique for characterizing the function of plant genes. VIGS is rapid, efficient and specific system for transient gene silencing. The major steps in VIGS includes; engineering viral genomes to the appropriate viral vector to incorporate fragments of host genes that are targeted to be silenced, infecting the appropriate plant hosts and silencing the target genes as part of the defense mechanism of the plant against virus infection. The VIGS vector is a recombinant virus engineered to be able to carry a piece an endogenous gene from the host. During infection with the modified vector, the host's defense reaction will be induced against the cloned host gene; a loss of function phenotype makes it possible to identify the function of the gene. The recombinant virus is introduced into plant cells through Agrobacterium tumefaciens mediated transient expression or in vitro transcribed RNA inoculation or direct DNA inoculation. The Trans gene is amplified along with the viral RNA by RNA dependent RNA polymerase generating dsRNA molecules. dsRNA is the triggering molecule of Post transcriptional gene silencing. VIGS as a reverse genetics tool for functional genomics studies presenting several advantages. Despite its great potential, many limitations remain to be overcome. In this review, the molecular mechanism in VIGS technology, its advanced application in plant functional genomics studies and the major limitation and potential future prospects were briefly discussed.Lack of an efficient method for virus vector delivery is another challenge in application of VIGS in plants. Agrobacterium mediated delivery of binary VIGS vectors is efficiently used in many dicot plants.The application of VIGS has now become more versatile. However, studies related to heritable and long duration VIGS have yet to be extended to other genes, apart from marker genes, in a wide range of plant species. The potential application of VIGS in crop improvement has yet to be realized. In light of recent advances in VIGS technology,

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“…It favors to study gene functions when mutations are lethal to the embryonic stages and resulting into deformation of the plants (26,32). It is also good for high-throughput genetic analysis (33)(34)(35), as it can be performed in a short time without any alteration in the plant genome (36,37).…”

Section: Introductionmentioning

confidence: 99%

Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana

Ali

Nasir

Rafiq

et al. 2017

Iran. J. Biotechnol.

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Background: Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives: pBINTRA6 vector was modifi ed by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1) and the two nonstructural proteins of TRV2(RNA2). Materials and Methods: SCMV construct was inoculated into 3-4 weeks Nicotiana benthamiana plant leaves either by using a needleless syringe or applying pricking with a toothpick. Results: The construct (SCMV-RNA2) successfully induced post-transcriptional gene silencing (PTGS) of the target genes GFP and ChlI through agroinoculation proving that SCMV is a substitute of the RNA1, which plays a pivotal role in the systemic gene silencing. 2-3-weeks of post inoculation, target genes' silencing was observed in the newly developed noninoculated leaves. Conclusions:The newly developed construct expresses the knocked down of the endogenous as well as exogenous genes and only four weeks are required for the transient expression of the gene silencing based on SCMV-VIGS system.

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Recent Advances on Virus Induced Gene Silencing (VIGS): Plant Functional Genomics

Abstract: Figure 1: General outline of 'VIGS' approach applied for plant functional genomics study.

Cited by 11 publications

References 15 publications

DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing

DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing

Applications of Virus Induced Gene Silencing (VIGS) in Plant Functional Genomics Studies

Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana

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