Virus-induced gene silencing unravels multiple transcription factors involved in floral growth and development in Phalaenopsis orchids

Hsieh, Ming Hsien; Pan, Zhao Jun; Lai, Pei Han; Lu, Hsiao‐Chi; Yeh, Hsin Hung; Hsu, Chia Chi; Wu, Wen-Chung; Chung, Mei Chu; Wang, Shyh Shyan; Chen, Wen Huei; Chen, Hong Hwa

doi:10.1093/jxb/ert218

Cited by 39 publications

(34 citation statements)

References 47 publications

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“…have a similar micromorphological structure as the sepals (Hsieh et al, 2013a(Hsieh et al, , 2013bPan et al, 2014). Similarly, the VIGS phenotypes of petals resembled those of sepals for changed pigmentation patterning on silencing of these PeMYBs, although much weaker (Supplemental Fig.…”

Section: Distinct Pigmentation Patterning In the Sepals/petals Withinmentioning

confidence: 73%

“…lip is a modified petal, its micromorphological structure is distinct from that of petals (Hsieh et al, 2013b;Pan et al, 2014). In the VIGS experiment, only PeMYB12-silenced lips showed reduced Figure 5.…”

Section: Distinct Pigmentation Patterning In the Lip Regulated By Pemmentioning

confidence: 95%

“…TF15 (PhaTF15) on disease resistance (Lu et al, 2012), Phalaenopsis equestris UDP glucose: flavonoid 3-O-glucosyltransferase (PeUFGT3) on anthocyanin biosynthesis , P. equestris MADS5 (PeMADS5), PeMADS6, P. equestris SEPALLATA1 (PeSEP1), PeSEP2, PeSEP3, and PeSEP4 on floral morphogenesis (Hsieh et al, 2013a;Pan et al, 2014), and high-throughput silencing of 126 transcription factors for identifying genes involved in flower development of Phalaenopsis spp. (Hsieh et al, 2013b).…”

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confidence: 99%

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Three R2R3-MYB Transcription Factors Regulate Distinct Floral Pigmentation Patterning in Phalaenopsis spp.

Hsu¹,

Chen²,

et al. 2015

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Orchidaceae are well known for their fascinating floral morphologic features, specialized pollination, and distinctive ecological strategies. With their long-lasting flowers of various colors and pigmentation patterning, Phalaenopsis spp. have become important ornamental plants worldwide. In this study, we identified three R2R3-MYB transcription factors PeMYB2, PeMYB11, and PeMYB12. Their expression profiles were concomitant with red color formation in Phalaenopsis spp. flowers. Transient assay of overexpression of three PeMYBs verified that PeMYB2 resulted in anthocyanin accumulation, and these PeMYBs could activate the expression of three downstream structural genes Phalaenopsis spp. Flavanone 3-hydroxylase5, Phalaenopsis spp. Dihydroflavonol 4-reductase1, and Phalaenopsis spp. Anthocyanidin synthase3. In addition, these three PeMYBs participated in the distinct pigmentation patterning in a single flower, which was revealed by virus-induced gene silencing. In the sepals/petals, silencing of PeMYB2, PeMYB11, and PeMYB12 resulted in the loss of the full-red pigmentation, red spots, and venation patterns, respectively. Moreover, different pigmentation patterning was regulated by PeMYBs in the sepals/petals and lip. PeMYB11 was responsive to the red spots in the callus of the lip, and PeMYB12 participated in the full pigmentation in the central lobe of the lip. The differential pigmentation patterning was validated by RNA in situ hybridization. Additional assessment was performed in six Phalaenopsis spp. cultivars with different color patterns. The combined expression of these three PeMYBs in different ratios leads to a wealth of complicated floral pigmentation patterning in Phalaenopsis spp.

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Section: Distinct Pigmentation Patterning In the Sepals/petals Withinmentioning

confidence: 73%

Section: Distinct Pigmentation Patterning In the Lip Regulated By Pemmentioning

confidence: 95%

mentioning

confidence: 99%

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Three R2R3-MYB Transcription Factors Regulate Distinct Floral Pigmentation Patterning in Phalaenopsis spp.

Hsu¹,

Chen²,

et al. 2015

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“…The chimeric repressor of SRDX has been used successfully to reduce C-class gene expression and produce double-flowered mutants in morning glory and cyclamen (Sage-Ono et al, 2011;Tanaka et al, 2013). Recently, virus-induced gene silencing (VIGS) has been employed to reduce B-or C-class gene expression in ornamental plants such as Aquilegia, Phalaenopsis, and Petunia Hsieh et al, 2013;Kramer et al, 2007;Noor et al, 2014). VIGS may be useful to create viridiflora and double-flower cultivars by reducing B-and C-class gene expression, respectively.…”

Section: Future Prospectsmentioning

confidence: 99%

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Kanno

2016

The Hortic J

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Molecular and genetic analyses of flower development have been conducted primarily in dicot model plants such as Arabidopsis thaliana and Antirrhinum majus. The obtained data are the basis for the ABC model, which was extended to the ABCE model of floral development. This model has been validated in many dicot species using genetic transformation studies and mutant analyses. Many dicot flowers have two distinctive perianth whorls, which include greenish sepals and showy petals. By contrast, the monocot lily flower has two almost identical petaloid whorls, the inner and outer tepals. To explain this type of floral morphology, a modified ABC model, the further extended modified ABCE model, was proposed. According to this model, B-class genes are expressed in whorls 1-3, and whorls 1 and 2 form petaloid structures. This review describes the molecular mechanisms regulating flower development in monocots. Since the showy perianth is one of the most important traits in floricultural crops, we focused on the B-and C-class genes, which are related to the development and modification of the perianth. The review describes the expression patterns of floral organ identity genes, and presents functional studies using double-flowered and viridiflora cultivars in some monocot species. Besides lily-type flowers, there are several types of monocot flower, such as commelina type with two whorls of distinctive perianth, orchid and grass flowers. The review also describes the molecular analyses of these types of monocot flower.

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“…Five transcription factors involved in various aspects of flower morphogenesis in the orchid Phalaenopsis equestris namely PeMADS1 (AGAMOUS-like MADS-box gene), PeMADS7 (D-class MADS-box gene), PeHB, PebHLH and PeZIP were inserted into orchid viruses, CymMV to explore their roles during flower development. Silencing of PeMADS1 and PebHLH gene lead to reduced flower size together with a petaloid column containing petallike epidermal cells and alterations of epidermal cell arrangement in lip lateral lobes, respectively, whereas silenced PeMADS7, PeHB, and PeZIP genes alone resulted in abortion of the first three fully developed flower buds of an inflorescence [15].…”

Section: Recent Advances On Vigs In Plant Functional Studymentioning

confidence: 99%

Recent Advances on Virus Induced Gene Silencing (VIGS): Plant Functional Genomics

Gupta¹

2013

J Plant Biochem Physiol

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Virus-induced gene silencing unravels multiple transcription factors involved in floral growth and development in Phalaenopsis orchids

Cited by 39 publications

References 47 publications

Three R2R3-MYB Transcription Factors Regulate Distinct Floral Pigmentation Patterning in Phalaenopsis spp.

Three R2R3-MYB Transcription Factors Regulate Distinct Floral Pigmentation Patterning in Phalaenopsis spp.

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Recent Advances on Virus Induced Gene Silencing (VIGS): Plant Functional Genomics

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