Virus-Induced Gene Silencing of Endogenous Genes and Promotion of Flowering in Soybean by Apple latent spherical virus-Based Vectors

Yamagishi, Noriko; Yoshikawa, Nobuyuki

doi:10.5772/14568

Cited by 7 publications

(1 citation statement)

References 49 publications

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“…However, VIGS technology also has its limitations. Due to the limitation of host range, different host plants may need to select corresponding virus vectors, and different virus vectors may have different requirements for the length of inserted exogenous gene fragments [8]. In addition, the sensitivity of plant materials to virus vectors also determines the method of introducing virus vectors suitable for use in plant materials.…”

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confidence: 99%

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial <em>Rosa</em> Plants under Field Conditions

Sui¹,

Yan²,

Han³

et al. 2018

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Virus-induced gene silencing (VIGS) technique, which is developed in recent years, is a rapid identification of plant gene function from reverse genetics. It is a manifestation of post-transcriptional gene silencing mechanism. Compared with the traditional transgenic technology, VIGS is a transient expression system, which can achieve good results in a short time. At present, it is widely used to study the function of plant genes, but most of them are model plants, and the experiments are carried out always in the indoor environment with controlled light and temperature conditions. In this study, we creatively provided a method to establish VIGS system using perennial Rosa plants as experimental materials under field conditions. The recombinant virus vector was constructed with RrGT1 gene as reporter gene and modified TRV-GFP virus as vector, and the perennial R. rugosa "Zizhi" and R. davurica were used as experimental verification materials. According to the growth conditions of Rosa plants, the natural environment in the field and the optimal conditions for the occurrence of VIGS, the technical problems such as the confirmation of the inoculation period, the preparation of the infective fluid, the inoculation technology of the virus vector and the light and temperature conditions of plant materials cultured after inoculation were solved one by one. When the RrGT1 gene was silenced, the Rosa plants showed a pale petal color phenotype. By detection, it was found that the expression of endogenous RrGT1 gene was significantly down-regulated, and the content of all anthocyanins also decreased significantly. Therefore, we believed that the attempt to establish VIGS system in perennial Rosa plants under field conditions was very successful. 1. INTRODUCTION Many plants in the genus Rosa, such as Rosa rugosa, Rosa chinensis and Rosa davidiana, have great

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confidence: 99%

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial <em>Rosa</em> Plants under Field Conditions

Sui¹,

Yan²,

Han³

et al. 2018

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Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Whitham

Eggenberger

Zhang

et al. 2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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Gaining insight into soybean defense responses using functional genomics approaches: Figure 1

Liu¹,

Graham²,

Pedley³

et al. 2015

Briefings in Functional Genomics

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Soybean pathogens significantly impact yield, resulting in over $4 billion dollars in lost revenue annually in the United States. Despite the deployment of improved soybean cultivars, pathogens continue to evolve to evade plant defense responses. Thus, there is an urgent need to identify and characterize gene networks controlling defense responses to harmful pathogens. In this review, we focus on major advances that have been made in identifying the genes and gene networks regulating defense responses with an emphasis on soybean-pathogen interactions that have been amenable to gene function analyses using gene silencing technologies. Further we describe new research striving to identify genes involved in durable broad-spectrum resistance. Finally, we consider future prospects for functional genomic studies in soybean and demonstrate that understanding soybean disease and stress tolerance will be expedited at an unprecedented pace.

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Virus-Induced Gene Silencing of Endogenous Genes and Promotion of Flowering in Soybean by Apple latent spherical virus-Based Vectors

Cited by 7 publications

References 49 publications

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial <em>Rosa</em> Plants under Field Conditions

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial <em>Rosa</em> Plants under Field Conditions

Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Gaining insight into soybean defense responses using functional genomics approaches: Figure 1

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Virus-Induced Gene Silencing of Endogenous Genes and Promotion of Flowering in Soybean by Apple latent spherical virus-Based Vectors

Cited by 7 publications

References 49 publications

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial &lt;em&gt;Rosa&lt;/em&gt; Plants under Field Conditions

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial &lt;em&gt;Rosa&lt;/em&gt; Plants under Field Conditions

Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

Gaining insight into soybean defense responses using functional genomics approaches: Figure 1

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Product

Resources

About

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial <em>Rosa</em> Plants under Field Conditions

Establishment of Virus-Induced Gene Silencing (VIGS) System in Perennial <em>Rosa</em> Plants under Field Conditions