Virus‐Engineered Colloidal Particles—A Surface Display System

Fischlechner, Martin; Toellner, Lars; Messner, Paul; Grabherr, Reingard; Donath, Edwin

doi:10.1002/anie.200502620

Cited by 46 publications

(23 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This strategy was applied to ensure co‐delivery of both entities to the same cell. So far, lipid layers were deposited on LbL capsules for various purposes, for example to modulate the LbL shell permeability for slow release of encapsulated substances,25 to coat them with viral lipids for bead‐based multiplex assays 26, 27. The potential of lipid‐coated microcapsules for antigen delivery has not been investigated yet.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Dendritic Cells by Lipid Grafted Polyelectrolyte Microcapsules

Temmerman

Rejman

Lucas

et al. 2012

Adv Funct Materials

View full text Add to dashboard Cite

Polyelectrolyte microcapsules are fabricated by layer-by-layer deposition of dextran sulfate and poly-L -arginine layers at the surface of calcium carbonate template microparticles followed by core removal to produce hollow microcapsules. In the context of vaccination, these biodegradable LbL capsules emerge as promising antigen carriers and are believed to have potential for the co-delivery of antigens and immunomodulators associated within the same particle to enhance and steer the type of immune response. To this end, it is shown that LbL microcapsules can be functionalized at their surface with lipid layers containing immunopotentiators of lipid nature. The potency of the different lipid modifi ed microcapsules to activate dendritic cells (DCs) is demonstrated by increased expression levels of the migration marker CCR7 and the maturation markers CD40 and CD86. Additionally, the DCs cytokine secretion profi le is evaluated. The fi ndings reveal that the lipid grafted microcapsules are superior to non-modifi ed microcapsules in DC activation and suggest their potential as immune modulating antigen delivery systems.

show abstract

Section: Discussionmentioning

confidence: 99%

Modulation of Dendritic Cells by Lipid Grafted Polyelectrolyte Microcapsules

Temmerman

Rejman

Lucas

et al. 2012

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…VERO cells showed an enhanced uptake of virus-coated PMLCs compared to lipid-coated PMLCs without virus particles. [158][159][160][161] Such virus-functionalized particles may find applications in diagnostics, vaccination, and gene delivery. For example, Toellner et al described a bead assay based on virus-functionalized PMLCs for the simultaneous detection of viral antibodies in serum.…”

Section: Reviewsmentioning

confidence: 99%

Polymeric Multilayer Capsules in Drug Delivery

et al. 2010

View full text Add to dashboard Cite

Recent advances in medicine and biotechnology have prompted the need to develop nanoengineered delivery systems that can encapsulate a wide variety of novel therapeutics such as proteins, chemotherapeutics, and nucleic acids. Moreover, these delivery systems should be "intelligent", such that they can deliver their payload at a well-defined time, place, or after a specific stimulus. Polymeric multilayer capsules, made by layer-by-layer (LbL) coating of a sacrificial template followed by dissolution of the template, allow the design of microcapsules in aqueous conditions by using simple building blocks and assembly procedures, and provide a previously unmet control over the functionality of the microcapsules. Polymeric multilayer capsules have recently received increased interest from the life science community, and many interesting systems have appeared in the literature with biodegradable components and biospecific functionalities. In this Review we give an overview of the recent breakthroughs in their application for drug delivery.

show abstract

“…Recently, bioactive adenoviral vectors embedded in multilayered polyelectrolyte films on a flat surface were shown to efficiently transfect different cell lines. [62] Fischlechner et al also suggested virus coating of LbLcoated colloids [63,64] and introduced these materials for use in multiplex suspension arrays to detect virus specific antibodies. [65] Hobson et al proposed using the extremely tight interaction between streptavidin and biotin to immobilize adenoviral vectors on the surface of wells and microparticles.…”

Section: Transduction Of the Cells On The Microcarriers By Adenoviralmentioning

confidence: 99%

Evaluation of Digitally Encoded Layer‐by‐layer Coated Microparticles as Cell Carriers

Fayazpour¹,

Lucas²,

Vandenbroucke³

et al. 2008

Adv Funct Materials

View full text Add to dashboard Cite

To obtain more biologically relevant data there is a growing interest in the use of living cells for assaying the biological activity of unknown chemical compounds. Density ‘multiplex’ cell‐based assays, where different cell types are mixed in one well and simultaneously investigated upon exposure to a certain compound are beginning to emerge. To be able to identify the cells they should be attached to microscopic carriers that are encoded. This paper investigates how digitally encoded microparticles can be loaded with cells while keeping the digital code in the microcarriers readable. It turns out that coating the surface of the encoded microcarriers with polyelectrolytes using the layer‐by‐layer (LbL) approach provides the microcarriers with a ‘highly functional’ surface. The polyelectrolyte layer allows the growth of the cells, allows the orientation of the cell loaded microcarriers in a magnetic field, and does not hamper the reading of the code. It has further been shown that the cells growing on the polyelectrolyte layer can become transduced by adenoviral particles hosted by the polyelectrolyte layer. It is concluded that the digitally encoded microparticles are promising materials for use in biomedical and pharmaceutical in‐vitro research where cells are used as tools.

show abstract

Virus‐Engineered Colloidal Particles—A Surface Display System

Cited by 46 publications

References 30 publications

Modulation of Dendritic Cells by Lipid Grafted Polyelectrolyte Microcapsules

Modulation of Dendritic Cells by Lipid Grafted Polyelectrolyte Microcapsules

Polymeric Multilayer Capsules in Drug Delivery

Evaluation of Digitally Encoded Layer‐by‐layer Coated Microparticles as Cell Carriers

Contact Info

Product

Resources

About