Virus‐Based Chemical and Biological Sensing

Mao, Chuanbin; Liu, Aihua; Cao, Binrui

doi:10.1002/anie.200900231

Cited by 246 publications

(197 citation statements)

References 193 publications

(208 reference statements)

Supporting

Mentioning

197

Contrasting

Order By: Relevance

“…The M13 phage proved useful in detecting target materials with high selectivity and sensitivity, because target-specific phage can be identified through well-established phage display techniques [36][37][38] . 4a) 39 .…”

Section: Resultsmentioning

confidence: 99%

Biomimetic virus-based colourimetric sensors

et al. 2014

View full text Add to dashboard Cite

Many materials in nature change colours in response to stimuli, making them attractive for use as sensor platform. However, both natural materials and their synthetic analogues lack selectivity towards specific chemicals, and introducing such selectivity remains a challenge. Here we report the self-assembly of genetically engineered viruses (M13 phage) into targetspecific, colourimetric biosensors. The sensors are composed of phage-bundle nanostructures and exhibit viewing-angle independent colour, similar to collagen structures in turkey skin. On exposure to various volatile organic chemicals, the structures rapidly swell and undergo distinct colour changes. Furthermore, sensors composed of phage displaying trinitrotoluene (TNT)-binding peptide motifs identified from a phage display selectively distinguish TNT down to 300 p.p.b. over similarly structured chemicals. Our tunable, colourimetric sensors can be useful for the detection of a variety of harmful toxicants and pathogens to protect human health and national security.

show abstract

Section: Resultsmentioning

confidence: 99%

Biomimetic virus-based colourimetric sensors

et al. 2014

View full text Add to dashboard Cite

show abstract

“…There remains an additional source of velocity fluctuations whose origin is not yet properly understood. Finally, we speculate that the labelfree detection of fd might have practical applications, as fd virus is a marker of sewage contamination in ground water 47 , and of E. coli contamination of the food supply 48 .…”

Section: Discussionmentioning

confidence: 99%

Stiff filamentous virus translocations through solid-state nanopores

et al. 2014

View full text Add to dashboard Cite

The ionic conductance through a nanometer-sized pore in a membrane changes when a biopolymer slides through it, making nanopores sensitive to single molecules in solution. Their possible use for sequencing has motivated numerous studies on how DNA, a semiflexible polymer, translocates nanopores. Here we study voltage-driven dynamics of the stiff filamentous virus fd with experiments and simulations to investigate the basic physics of polymer translocations. We find that the electric field distribution aligns an approaching fd with the nanopore, promoting its capture, but it also pulls fd sideways against the membrane after failed translocation attempts until thermal fluctuations reorient the virus for translocation. fd is too stiff to translocate in folded configurations. It therefore translocates linearly, exhibiting a voltage-independent mobility and obeying first-passage-time statistics. Surprisingly, lengthwise Brownian motion only partially accounts for the translocation velocity fluctuations. We also observe a voltage-dependent contribution whose origin is only partially determined.

show abstract

“…Additionally, bacterial surface display has been used to discover a small peptide employed for targeted ultrasound (Brown et al 2000;Weller et al 2005). In another emerging area, phage engineered to interact with nanomaterials offer great promise for in vivo imaging applications (Petrenko 2008;Mao et al 2009). For example, self-assembling hydrogels composed of phage and gold nanoparticles have recently been reported (Souza et al 2006;Souza et al 2008).…”

Section: Horizonsmentioning

confidence: 99%

Phage display and molecular imaging: expanding fields of vision in living subjects

Cochran

2010

Biotechnology and Genetic Engineering Reviews

View full text Add to dashboard Cite

In vivo molecular imaging enables non-invasive visualization of biological processes within living subjects, and holds great promise for diagnosis and monitoring of disease. The ability to create new agents that bind to molecular targets and deliver imaging probes to desired locations in the body is critically important to further advance this field. To address this need, phage display, an established technology for the discovery and development of novel binding agents, is increasingly becoming a key component of many molecular imaging research programs. This review discusses the expanding role played by phage display in the field of molecular imaging with a focus on in vivo applications. Furthermore, new methodological advances in phage display that can be directly applied to the discovery and development of molecular imaging agents are described. Various phage library selection strategies are summarized and compared, including selections against purified target, intact cells, and ex vivo tissue, plus in vivo homing strategies. An outline of the process for converting polypeptides obtained from phage display library selections into successful in vivo imaging agents is provided, including strategies to optimize in vivo performance. Additionally, the use To whom correspondence may be addressed (cochran1@stanford.edu) Abbreviations: scFv, single chain variable fragment; Fab, fragment antigen binding; ELISA, Enzyme-Linked Immunosorbent Assay; NEB, New England Biolabs; PET, positron emission tomography; SPECT, single photon emission computed tomography; NIR, near infrared; PEG, polyethylene glycol; SPARC, secreted protein acidic and rich in cysteine; VCAM-1, vascular cell adhesion molecule; MRI, magnetic resonance imaging; DOTA, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; IC 50 , half maximal inhibitory concentration; CT, computed tomography; K D , equilibrium dissociation constant; EGFR, epidermal growth factor receptor; EGFR-ECD, EGFR extracellular domain; Aβ 42 , amyloid-beta; MMP, matrix metalloprotease; uPAR, urokinase-type plasminogen activator receptor; VEGF, vascular endothelial growth factor; IL-11, Interleukin-11; IL-11αR, Interleukin-11 α-receptor. 58F.V. CoChran and J.r. CoChran of phage particles as imaging agents is also described. In the latter part of the review, a survey of phage-derived in vivo imaging agents is presented, and important recent examples are highlighted. Other imaging applications are also discussed, such as the development of peptide tags for site-specific protein labeling and the use of phage as delivery agents for reporter genes. The review concludes with a discussion of how phage display technology will continue to impact both basic science and clinical applications in the field of molecular imaging.

show abstract

Virus‐Based Chemical and Biological Sensing

Cited by 246 publications

References 193 publications

Biomimetic virus-based colourimetric sensors

Biomimetic virus-based colourimetric sensors

Stiff filamentous virus translocations through solid-state nanopores

Phage display and molecular imaging: expanding fields of vision in living subjects

Contact Info

Product

Resources

About