Virus and virus-like particles observed in the intestinal contents of the possum,Trichosurus vulpecula

Rice, Maxine; Wilks, C. R.

doi:10.1007/bf01718168

Cited by 16 publications

(14 citation statements)

References 15 publications

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“…These viruses would either be pathogenic to brushtail possums, or suitable as vectors for the delivery of contraceptive antigens. An electron microscopy (EM) survey of brushtail possum intestinal contents (Rice and Wilks, 1996) revealed the presence of four types of viruses: adenoviruses, herpesviruses, coronaviruses and coronavirus-like particles. Other reports of viruses of the brushtail possum include a papillomavirus (Perrott et al, 2000) and a retrovirus (Baillie and Wilkins, 2001).…”

mentioning

confidence: 99%

Molecular confirmation of an adenovirus in brushtail possums (Trichosurus vulpecula)

2002

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Partial genome characterisation of a non-cultivable marsupial adenovirus is described. Adenovirus-like particles were found by electron microscopy (EM) in the intestinal contents of brushtail possums (Trichosurus vulpecula) in New Zealand. Using degenerate PCR primers complementary to the most conserved genome regions of adenoviruses, the complete nucleotide sequence of the penton base gene, and partial nucleotide sequences of the DNA polymerase, hexon, and pVII genes were obtained. Phylogenetic analysis of the penton base gene strongly suggested that the brushtail possum adenovirus (candidate PoAdV-1) belongs to the recently proposed genus Atadenovirus. Sequence analysis of the PCR products amplified from the intestinal contents of brushtail possums originating from different geographical regions of New Zealand identified a single genotype. This is the first report of molecular confirmation of an adenovirus in a marsupial.

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mentioning

confidence: 99%

Molecular confirmation of an adenovirus in brushtail possums (Trichosurus vulpecula)

2002

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“…In Australian marsupials, electron microscopy and serologic surveys have detected evidence of herpesvirus infection in possums (Trichosurus vulpecula), dasyurids (Phascogale tapoatafa and Antechinus stuartii), and wombats (Vombatus ursinus), but no sequence data from these viruses have been reported (Webber and Whalley, 1978;Barker et al, 1981;Rothwell et al, 1988;Rice and Wilks, 1996). Most recently, two novel gammaherpesviruses (phascolarctid herpesviruses 1 and 2, PhaHV-1 and PhaHV-2) have been detected in wild koalas (Phascolarctos cinereus) by partial sequencing of the conserved region of the DNA polymerase gene (Vaz et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Novel Herpesvirus From a Free-Ranging Eastern Grey Kangaroo (Macropus Giganteus)

Vaz

Motha

McCowan

et al. 2013

Journal of Wildlife Diseases

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ABSTRACT:We isolated a macropodid herpesvirus from a free-ranging eastern grey kangaroo (Macropus giganteous) displaying clinical signs of respiratory disease and possibly neurologic disease. Sequence analysis of the herpesvirus glycoprotein G (gG) and glycoprotein B (gB) genes revealed that the virus was an alphaherpesvirus most closely related to macropodid herpesvirus 2 (MaHV-2) with 82.7% gG and 94.6% gB amino acid sequence identity. Serologic analyses showed similar cross-neutralization patterns to those of MaHV-2. The two viruses had different growth characteristics in cell culture. Most notably, this virus formed significantly larger plaques and extensive syncytia when compared with MaHV-2. No syncytia were observed for MaHV-2. Restriction endonuclease analysis of whole viral genomes demonstrated distinct restriction endonuclease cleavage patterns for all three macropodid herpesviruses. These studies suggest that a distinct macropodid alphaherpesvirus may be capable of infecting and causing disease in eastern grey kangaroos.

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“…No suitable candidates for development as possuminfective agents were isolated from possums in Australia, but adenovirus, herpesvirus, coronavirus-like virus, papillomavirus, enterovirus and an indigenous type D retrovirus isolates were recorded from possums in New Zealand (Rice & Wilks 1996;Perrott et al 2000b;Baillie & Wilkins 2001;Zheng 2007). Additionally, a novel possum-infective agent was identified to cause wobbly possum disease (WPD), a disease recorded in both captivity and the wild in New Zealand (Mackintosh et al 1995;Perrott et al 2000a).…”

Section: Species-specific Infectious Agents As Transmissible Vectors:mentioning

confidence: 99%

Could recombinant technology facilitate the realisation of a fertility-control vaccine for possums?

Cross

Zheng

Duckworth

et al. 2011

New Zealand Journal of Zoology

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While lethal control remains the primary tool for possum control in New Zealand, there has been substantial research effort over the last decade into controlling possum populations by reducing the animals' breeding potential. In particular, the potential of immunological control to reduce fertility has been investigated, whereby the possums' immune system is induced to react against its reproductive system, in order to block or destroy key components of the reproductive process. Two of the most targeted key components have been the blocking of circulating hormones (e.g. gonadotrophin-releasing hormone) and the inhibition of functional egg surface proteins (e.g. zona pellucida). While some success has been achieved for each approach, three main obstacles remain to the development of a working fertility-control vaccine for possums: first, ensuring that the vaccine remains efficacious in oral-delivery formulation; second, ensuring sufficient levels of fertility reduction; and third, ensuring that the induced immune response is sustained for a duration sufficient for long-term suppression of reproduction. The use of a genetically modified recombinant organism (parasite, bacterium or virus) to deliver a fertility-control vaccine could satisfy these requirements. A strong precedent for this approach has been set already in wildlife biology, namely the oral rabies vaccine, which is based on a recombinant vaccinia virus and which has been used successfully for the last 25 years as a disease-eliminating vaccine in mesocarnivores in Europe and North America. This review outlines the different forms and examples of recombinant organisms with potential for engineering into recombinant fertilitycontrol vaccines to reduce possum reproduction; non-transmissible agents or fully-transmissible vectors are discussed, with shortcomings and benefits outlined for each.

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Virus and virus-like particles observed in the intestinal contents of the possum,Trichosurus vulpecula

Cited by 16 publications

References 15 publications

Molecular confirmation of an adenovirus in brushtail possums (Trichosurus vulpecula)

Molecular confirmation of an adenovirus in brushtail possums (Trichosurus vulpecula)

Isolation and Characterization of a Novel Herpesvirus From a Free-Ranging Eastern Grey Kangaroo (Macropus Giganteus)

Could recombinant technology facilitate the realisation of a fertility-control vaccine for possums?

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