Virulence traits and antibiotic resistance among enterococci isolated from Eurasian otter (Lutra lutra)

Semedo-Lemsaddek, Teresa; Nóbrega, Cláudia S.; Ribeiro, Tânia; Pedroso, Nuno M.; Sales-Luís, Teresa; Lemsaddek, Abdelhak; Tenreiro, Rogério; Tavares, Luís; Vilela, C. L.; Oliveira, Manuela

doi:10.1016/j.vetmic.2012.12.032

Cited by 36 publications

(31 citation statements)

References 20 publications

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“…occurrence, colonisation, counts, sensitivity to ATBs and other antimicrobials, probiotic features and antimicrobial activities), there are still some new attributes/information which are important not only from the basic research point of view but also from the protection possibility aspect and those attributes are also virulence determinants. While the virulence traits in free living animals (Eurasian otters and beavers) have already been described (Semedo- Lemsaddek et al, 2013;Lauková et al, 2015), the detection of virulence factor genes in enterococci from rabbits as food-producing animals had never been tested before. Virulence factor genes were also tested in dogs and cats (Iseppi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In free-living animals this gene was also absent (Semedo-Lemsaddek et al, 2013;Lauková et al, 2015). There was a low prevalence of the esp gene in rabbit isolates (10%); in beavers this gene was detected only in one strain (Lauková et al, 2015) and strains from otter were esp gene absent (Semedo-Lemsaddek et al, 2013), similar to those from dogs and cats (Iseppi et al, 2015). The esp gene occurs mainly in clinical isolates (Medeiros et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Haemolysis was performed by streaking the strains on Columbia Agar (Oxoid, Basingstoke, United Kingdom) supplemented with 5% defibrinated sheep blood (Semedo-Lemsaddek et al, 2003, 2013. In the gelatinase assay, enterococci were inoculated on Todd-Hewitt agar (Becton and Dickinson) supplemented with gelatine (30 g/L, Becton and Dickinson) and incubated at 37°C for 2 d. Then the plates were flooded with a 15% solution consisting of 15% HgCl 2 in 20% HCl.…”

Section: Detection Of Haemolysis and Gelatinase Activitymentioning

confidence: 99%

“…Moreover, some enterococci possess probiotic characteristics and can improve the microbial balance of the intestine and prevent gastrointestinal diseases in humans and animals (Franz et al, 1999;Foulquie Moreno et al, 2006;Strompfová et al, 2006;Pogány Simonová et al, 2009) which means they have to be individually evaluated. Besides their beneficial effects, enterococci are often recognised as nosocomial pathogens because of their increased resistance to antibiotics and the presence of virulence factor genes which ease bacterial adherence to the host cell membranes (Franz et al, 1999;Foulquie Moreno et al, 2006;Özmen Toğay et al, 2010;Semedo-Lemsaddek et al, 2013). Although a number of enterococcal virulence factors have been described, the range is still incomplete.…”

Section: Introductionmentioning

confidence: 99%

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Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins

Simonová

Lauková

2017

World Rabbit Sci.

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Information concerning the virulence factor genes and antibiotic resistance of rabbit enterococci is limited, so in this study we tested the virulence factor genes in Enterococcus faecalis strains from rabbits. Moreover, their resistance/sensitivity to antibiotics and sensitivity to enterocins was also tested, with the aim of contributing to our enterocin spectra study and to indicate the possibility of enterocin application in prevention or contaminant elimination in rabbit husbandry. A total of 144 rabbit samples were treated using a standard microbiological method. Thirty-one pure colonies of the species Enterococcus faecalis were identified, using the MALDI-TOF identification system and confirmed using phenotyping, among which 15 strains were virulence factor gene absent. The gelE gene was the most detected (42%); however, the expression of gelatinase phenotype did not always correlate with the detection of gelE. Strains did not show ß-haemolysis and were mostly resistant to tested antibiotics, but sensitive to enterocins (Ent), mainly to Ents EK13=A (P), 2019 and Ent M. Rabbit E. faecalis strains displayed antibiotic resistant traits and the presence of expressed and silent virulence genes, but they showed high levels of sensitivity to natural antimicrobials-enterocins, which indicates the possible prevention of multidrug and virulent enterococcal contaminants by enterocins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Detection Of Haemolysis and Gelatinase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins

Simonová

Lauková

2017

World Rabbit Sci.

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“…Plates were incubated at 37 o C for 24-48 h under semi-anaerobic conditions. The presence or absence of clearing zones around the colonies was interpreted as β-hemolysis and negative gama-hemolysis respectively (Semedo et al 2003(Semedo et al , 2013.…”

Section: Hemolysis Forming and Gelatinase Activitymentioning

confidence: 99%

Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Kandričáková¹,

Lauková²,

Strompfová³

2015

Polish Journal of Veterinary Sciences

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With an increasing number of pheasants as gamebirds being reared each year, these species are becoming a more prominent part of the workload of many veterinary practices. Only limited information can be found concerning the microflora of common pheasants. A significant part of the obligate microflora consists of lactic acid bacteria, including enterococci. In this study, faeces were sampled from 60 pheasants aged 16-17 weeks. Enterococcal counts reached 5.48±1.9 (log10) CFU/g. Strains (17) were taxonomically classified to the genus Enterococcus using the Maldi-Tof identification system; they were allotted to the species E. hirae (58.8%), E. faecium (23.5%) and E. faecalis (17.7%) by highly probable species identification or by secure genus identification/probable species identication. Species allocation was also confirmed using conventional biochemical tests. Most strains formed β-hemolysis. Gelatinase active phenotype was found in three E. faecalis strains. Enterococci were β-glucuronidase negative, mostly trypsin negative with slight or moderate production of α-chymotrypsin. EH52b and EF42 strains possessed the highest potential for pathogenicity. Average value of lactic acid was 1.78±0.33 mmo/L. Most strains were tetracycline resistant (82.4%). Polyresistant E. faecalis strains with positive gelatinase phenotype and possessing virulence factor genes confirmed using PCR (gelE, efaAfs, ccf cob, cpd) were sensitive to enterocins (activity 1600-25 600 AU/mL).

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Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain

et al. 2023

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Nowadays, developed more precisious identi cation techniques have allowed to identify and validate newer enterococcal species. Among those species, also the species Enterococcus moraviensis was validated, the representat of which was rstly isolated from surface waters. However, in this study, characteristic and bacteriocin potential of E. moraviensis strain EMo 1-1Nik isolated from buccal mucosa of Slovak warm-blood horse breed has been studied. BLASTn analysis allotted EMo 1-1Nik strain to the species E. moraviensis with percentage identity BLASTn 16S rRNA sequence in the strain up to 100% (99.93% similarity with E. moraviensis NR113937.1 strain). The EMo 1-1Nik strain has been provided with GenBank accession number MW326085 (for nucleotide sequence SUB8683960 Seq1). EMo 1-1Nik strain is hemolysis-negative (γ -hemolysis), DNase negative and gelatinase negative; absent of virulence factor genes, low-grade bio lm-positive (0.133 ± 0.36), mostly susceptible to tested antibiotics. Moreover, 60% of EMo1-1Nik colonies were found as bacteriocin-producing against the principal indicator Enterococcus avium EA5. EMo1-1Nik concentrated substance pH 4.5 (CS) showed the inhibitory activity against EA5 strain (200 AU/mL); CSs with pH 6.3 and 7.3 reached inhibitory activity 100 AU/mL against EA5 strain.Oppositelly, EMo 1-1Nik was susceptible to Mundticin EM41/3 (800 AU/mL) produced by fecal horses strain E. mundtii EM41/3. In spite of the preliminary results, it has been shown bacteriocin potential of safe E. moraviensis EMo1-1Nik strain. The additional studies are in processing.

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Virulence traits and antibiotic resistance among enterococci isolated from Eurasian otter (Lutra lutra)

Cited by 36 publications

References 20 publications

Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins

Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins

Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain

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