Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study

Bona, Mariana Duarte; Santos, Ana K. S.; Freitas, Thiago Miranda de; Prata, Mara A; Veras, Herlice N.; Amaral, Marília S. M. G.; Oliveira, Daniel Pereira de; Havt, Alexandre; Lima, Aldo Ângelo Moreira

doi:10.1016/j.ijmm.2019.01.003

Cited by 14 publications

(14 citation statements)

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“…In our study, the genes that were associated with specific symptoms in earlier studies [15,16], were not confirmed. In another study that was conducted in Brazil among children with shigellosis, sepA was associated with abdominal pain, and the combination of sepA, sigA Fig.…”

Section: Discussioncontrasting

confidence: 89%

“…They found that the sen (shET-2) gene was associated with diarrhea in children in general, but not with specific symptoms of shigellosis patients. They associated the virA gene with fever in children with shigellosis, however virA was also found in 44% of controls [15]. In our study, we have used a larger sample size consisting of patients with other demographics in another setting, analyzed all genes harbored instead of a predefined selection, used other methods with higher resolution as it was based on whole genomes, and included correction for multiple testing.…”

Section: Discussionmentioning

confidence: 99%

“…A few studies have investigated the association of virulence genes with disease severity for shigellosis, using Pearson's correlation and regression analyses [15,16]. In one of these studies, the virulence gene sepA was associated with abdominal pain and the combination of sepA, sigA and ial genes with bloody stools [16].…”

Section: Introductionmentioning

confidence: 99%

“…In one of these studies, the virulence gene sepA was associated with abdominal pain and the combination of sepA, sigA and ial genes with bloody stools [16]. Another study found that detection of the sen (shET-2) gene was associated with diarrhea and the virA gene was associated with fever [15]. Both studies had a limited sample number, did not correct for multiple testing, and in one study the presence of virulence genes was established using direct detection in fecal samples.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide association studies of Shigella spp. and Enteroinvasive Escherichia coli isolates demonstrate an absence of genetic markers for prediction of disease severity

et al. 2020

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Background: We investigated the association of symptoms and disease severity of shigellosis patients with genetic determinants of infecting Shigella and entero-invasive Escherichia coli (EIEC), because determinants that predict disease outcome per individual patient could be used to prioritize control measures. For this purpose, genome wide association studies (GWAS) were performed using presence or absence of single genes, combinations of genes, and k-mers. All genetic variants were derived from draft genome sequences of isolates from a multicenter cross-sectional study conducted in the Netherlands during 2016 and 2017. Clinical data of patients consisting of binary/dichotomous representation of symptoms and their calculated severity scores were also available from this study. To verify the suitability of the methods used, the genetic differences between the genera Shigella and Escherichia were used as control. Results: The isolates obtained were representative of the population structure encountered in other Western European countries. No association was found between single genes or combinations of genes and separate symptoms or disease severity scores. Our benchmark characteristic, genus, resulted in eight associated genes and > 3,000,000 k-mers, indicating adequate performance of the algorithms used. Conclusions: To conclude, using several microbial GWAS methods, genetic variants in Shigella spp. and EIEC that can predict specific symptoms or a more severe course of disease were not identified, suggesting that disease severity of shigellosis is dependent on other factors than the genetic variation of the infecting bacteria. Specific genes or gene fragments of isolates from patients are unsuitable to predict outcomes and cannot be used for development, prioritization and optimization of guidelines for control measures of shigellosis or infections with EIEC.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide association studies of Shigella spp. and Enteroinvasive Escherichia coli isolates demonstrate an absence of genetic markers for prediction of disease severity

et al. 2020

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“…инфекционные гемоколиты), опасны осложнениями и неблагоприятными исходами, поэтому представляют серьезную проблему здравоохранения [1][2][3]. В свою очередь, перенесенную кишечную инфекцию многие авторы рассматривают в качестве триггера при формировании гастроэнтерологической патологии функциональной и органической природы [4][5][6]. Показано, что риск развития патологии желудочно-кишечного тракта (ЖКТ) выше у детей, имеющих отягощенную наследственность, неблагополучный антенатальный анамнез, перинатальные поражения ЦНС, получавших раннее искусственное вскармливание, часто болеющих респираторными и аллергическими заболеваниями, перенесших повторные кишечные инфекции [7].…”

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Risk factors for the formation of gastroenterological pathology after infectious hemocolitis in children

Klimova¹,

Гончар

Lobzin

et al. 2020

Det. infekc.

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The aim of the study was to determine the risk factors for the formation of gastroenterological pathology in convalescents of infectious hemocolites. In the Department of intestinal infections, 120 children aged over 1 month who had intestinal infections with hemocolitis syndrome were observed. The etiology of the disease was verified, clinical and laboratory signs of intoxication, dehydration, local and systemic inflammatory response were detected. Non-specific complications (respiratory diseases, urinary tract infection) were diagnosed. In the acute period of the disease, the composition of the intestinal microbiota was evaluated by PCR-RT. During 6 months, 65 convalescents were observed in the catamnesis. If there were complaints from the gastrointestinal tract, functional gastrointestinal disorders (FGID) were diagnosed. When «symptoms of anxiety» appeared, organic intestinal lesions were detected. When «symptoms of anxiety» appeared, organic intestinal lesions were detected. The results were evaluated using the student's test, the Mann-Whitney U-test, the Kruskal-Wallis test, the Pearson correlation method, and discriminant analysis. Studies have shown that there were 43 children with a favorable outcome (66.2%), 17 children with FGID (26.1%), and 5 children with organic intestinal lesions (7.7%). In patients who formed organic lesions, the bacterial etiology of hemocolitis was more often noted. Gastroenterocolitis was more often diagnosed in patients with a favorable outcome (41.9%; p > 0,05). Non-specific complications were more often observed in patients who formed FGID and organic lesions. The frequency of FGID decreased with age and was higher in girls (58.8%) than in boys (41.2%) (p > 0,05). Organic intestinal lesions were observed only in girls. In convalescents with FGID, more often (83.3%) than in convalescents with a favorable outcome (64.3%), signs of anaerobic intestinal imbalance were detected. A complex of clinical and laboratory signs of an acute period of infectious hemocolites that determine the risk of FGID formation has been established. It was shown that the risk of developing FGID is higher in young convalescents, and the risk of forming organic intestinal lesions is higher in girls of preschool and school age. Patients who showed more pronounced systemic inflammatory responses in the acute period of the disease were more likely to have a favorable outcome.

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Molecular characterization of Shigella species isolated from diarrheal patients in Tehran, Iran: phylogenetic typing and its association with virulence gene profiles and a novel description of Shigella invasion associated locus

Arabshahi

Novinrooz

Ranjbar

et al. 2020

Eur J Clin Microbiol Infect Dis

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Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study

Cited by 14 publications

References 36 publications

Genome-wide association studies of Shigella spp. and Enteroinvasive Escherichia coli isolates demonstrate an absence of genetic markers for prediction of disease severity

Genome-wide association studies of Shigella spp. and Enteroinvasive Escherichia coli isolates demonstrate an absence of genetic markers for prediction of disease severity

Risk factors for the formation of gastroenterological pathology after infectious hemocolitis in children

Molecular characterization of Shigella species isolated from diarrheal patients in Tehran, Iran: phylogenetic typing and its association with virulence gene profiles and a novel description of Shigella invasion associated locus

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