Virulence of Vibrio harveyi responsible for the “Bright-red” Syndrome in the Pacific white shrimp Litopenaeus vannamei

Soto-Rodríguez, Sonia A.; Gómez‐Gil, Bruno; Lozano, Rodolfo; Río-Rodríguez, Rodolfo del; Diéguez, Ana L.; Romalde, Jesús L.

doi:10.1016/j.jip.2012.01.006

Cited by 78 publications

(42 citation statements)

References 60 publications

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“…When the infection has become systemic, hemocytes migrate to other tissues (antennal gland, heart, lymphoid organ, and striated muscle) to fight other bacteria (25,30). This response might explain why GCs were replaced by YCs in the TCBS as the infection progressed (unpublished data), which was observed in labo- ratory assays in which the pathogen strain M09-04 was used, and why YCs were predominant in shrimp from farms as well as in challenge assays during the terminal stage of the disease.…”

Section: Discussionmentioning

confidence: 99%

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Soto-Rodríguez

Gómez‐Gil

Lozano‐Olvera

et al. 2015

Appl Environ Microbiol

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308

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Moribund shrimp affected by acute hepatopancreatic necrosis disease (AHPND) from farms in northwestern Mexico were sampled for bacteriological and histological analysis. Bacterial isolates were molecularly identified as Vibrio parahaemolyticus by the presence of the tlh gene. The tdh-negative, trh-negative, and tlh-positive V. parahaemolyticus strains were further characterized by repetitive extragenic palindromic element-PCR (rep-PCR), and primers AP1, AP2, AP3, and AP and an ems2 IQ2000 detection kit (GeneReach, Taiwan) were used in the diagnostic tests for AHPND. The V. parahaemolyticus strains were used in immersion challenges with shrimp, and farmed and challenged shrimp presented the same clinical and pathological symptoms: lethargy, empty gut, pale and aqueous hepatopancreas, and expanded chromatophores. Using histological analysis and bacterial density count, three stages of AHNPD (initial, acute, and terminal) were identified in the affected shrimp. The pathognomonic lesions indicating severe desquamation of tubular epithelial cells of the hepatopancreas were observed in both challenged and pond-infected shrimp. The results showed that different V. parahaemolyticus strains have different virulences; some of the less virulent strains do not induce 100% mortality, and mortality rates also rise more slowly than they do for the more virulent strains. The virulence of V. parahaemolyticus strains was dose dependent, where the threshold infective density was 10 4 CFU ml ؊1 ; below that density, no mortality was observed. The AP3 primer set had the best sensitivity and specificity. Field and experimental results showed that the V. parahaemolyticus strain that causes AHPND acts as a primary pathogen for shrimp in Mexico compared with the V. parahaemolyticus strains reported to date.

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Section: Discussionmentioning

confidence: 99%

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Soto-Rodríguez

Gómez‐Gil

Lozano‐Olvera

et al. 2015

Appl Environ Microbiol

Self Cite

308

212

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“…V. harveyi is a saprophytic and opportunistic bacterium, living in fish culture environment in normal condition and becomes pathogen in worsening environmental and host condition (Garrity et al 2004). Vibriosis disease occurs in shrimp including in larvae stadia up to adult shrimp (Soto-Rodriguez et al 2012, Munaeni et al 2014, is antibiotic use (Van Hai et al 2015). Medicinal plant is considered to be able to show potential effect on the growth and survival and also has antimicrobial properties on aquatic organism Citarasu 2010).…”

Section: Abstract: Eleutherine Palmifolia Inhibitory Potential Phymentioning

confidence: 99%

In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi

et al. 2017

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B (40 mg mL ), C (60 mg mL ), D (80 mg mL ). Hasil uji fitokimia secara kualitatif menunjukkan bahwa ekstrak bawang hutan buton mengandung senyawa flavonoid, tanin, saponin, quinon, steroid dan triterpenoid. Uji daya hambat terbesar terdapat pada perlakuan D, sedangkan daya hambat minimum terdapat pada perlakuan A. Perlakuan terbaik dari uji kultur bersama juga ditemukan pada perlakuan D. Ekstrak bawang hutan buton pada penelitian ini dapat menghambat pertumbuhan V. harveyi.

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“…, larva postlarva 100% , (Gopal et al, 2005, Ruwandeepika et al, 2010, Saori et al, 2011, Soto-Rodriguez et al, 2012. Jung et al (2012Jung et al ( ) 2007Jung et al ( -2010 2,413 15.5-61.8%…”

Section: 모노퍼설페이트 화합물의 Vibrio Harveyi에 대한 살균력 및 횐다리새우(Litopenaeus Vannmentioning

confidence: 99%

Bactericidal Efficacy of a Monopersulfate Compound against Vibrio harveyi and Toxicity to Litopenaeus vannamei

Min¹,

Na²,

Chong³

et al. 2015

Korean Journal of Fisheries and Aquatic Sciences

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Vibrio harveyi is one of the most serious pathogens causing vibriosis in larval and grow-out shrimp culture. This study was performed to investigate the bactericidal effect of a monopersulfate compound against V. harveyi and its toxicity in Litopenaeus vannamei. The monopersulfate compound was prepared at 0, 0.15, 0.3, 0.6, 1.2, and 2.4 ppm for the bactericidal efficacy study, and then V. harveyi was added at a rate of 1×10 6 CFU/mL. Subsequently, five shrimps/dose were added to the water bath. The other groups of shrimp were exposed to monopersulfate, but not to the bacterium. None of the shrimps exposed to any of the monopersulfate treatment doses without bacteria died, and no changes in their movement were detected for 7 days. However, shrimps exposed to bacteria without monopersulfate showed decreased movement and lethargy, but no death. The total number of other bacteria and V. harveyi at the different concentrations decreased significantly by 4-6 log values compared to that in the bacterial control group. The monopersulfate compound completely inhibited V. harveyi growth beginning 1 h after exposure to 2.4 ppm. These results show that the monopersulfate compound is an efficient disinfectant against naturally occurring marine bacteria and V. harveyi without being toxic to shrimp.

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Virulence of Vibrio harveyi responsible for the “Bright-red” Syndrome in the Pacific white shrimp Litopenaeus vannamei

Cited by 78 publications

References 60 publications

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi

Bactericidal Efficacy of a Monopersulfate Compound against Vibrio harveyi and Toxicity to Litopenaeus vannamei

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