Virulence of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis in reconstituted human tissue models

Gácser, Attila; Schäfer, Wilhelm; Nosanchuk, Jerome S.; Salomon, Siegfried; Nosanchuk, Joshua D.

doi:10.1016/j.fgb.2007.02.002

Cited by 118 publications

(95 citation statements)

References 23 publications

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“…C. orthopsilosis is a newly defined species within the C. parapsilosis species complex [17]. However, differentiation within this species complex may be clinically irrelevant [17,18].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Montero

Shea

Jones

et al. 2008

Eur J Clin Microbiol Infect Dis

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Pyrosequencing was used to identify 133 isolates of clinically relevant non-dematiaceous yeasts. These included 97 ATCC strains (42 type strains), 7 UAMH strains, and 29 clinical isolates. Isolates belonged to the following genera: Candida (18 species), Trichosporon (10), Cryptococcus (7), Malassezia (3), Rhodotorula (2), Geotrichum (1), Blastoschizomyces (1) and Kodamaea (1). Amplicons were obtained of a hypervariable ITS region and analyzed using Pyrosequencing technology. The data were evaluated by a BLAST search against the GenBank database and correlated with data obtained by conventional cycle sequencing of the ITS1-5.8S-ITS2 region. Cycle sequencing identified (78.9%) if the isolates to the species level. Pyrosequencing technology identified 69.1%. In 90.1% of all the strains tested, identification results of both sequencing methods were identical. Most Candida isolates can be identified to the species level by pyrosequencing. Trichosporon species and some Cryptococcus cannot be differentiated at the species level. Pyrosequencing can be used for the reliable identification of most commonly isolated nondematiaceous yeasts, with a reduction of cost per identification compared to conventional sequencing.

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“…C. orthopsilosis is a newly defined species within the C. parapsilosis species complex [17]. However, differentiation within this species complex may be clinically irrelevant [17,18].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Montero

Shea

Jones

et al. 2008

Eur J Clin Microbiol Infect Dis

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“…C. parapsilosis is a common cause of bloodstream infection in neonates [3-5], and has overtaken C. albicans in frequency of hospital-acquired infection in some hospitals worldwide [4, 6, 7]. Recent studies have identified the importance of secreted lipase in the virulence of this organism and have documented its ability to damage tissue in vitro [8, 9]. …”

Section: Introductionmentioning

confidence: 99%

High efficiency opsonin-independent phagocytosis of Candida parapsilosis by human neutrophils

et al. 2009

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Candida species are associated with invasive fungal infections, and C. parapsilosis has become increasingly prevalent. As key antifungal effector cells, the function of human neutrophils confronting C. parapsilosis was investigated. We hypothesized that interaction between neutrophils and Candida species may not be uniform. Opsonins were omitted from these studies to understand the antifungal mechanisms at their most basic level. Human neutrophils underwent phagocytosis of C. parapsilosis with much higher efficiency than C. albicans. Immunofluorescence assays with β-glucan specific antibody detected more surface exposed β-glucan on C. parapsilosis than C. albicans. However, blockade of the β-glucan receptor, Dectin-1, reduced phagocytosis of C. albicans but not C. parapsilosis. Inclusion of excess β-glucan, mannan, or chitin also had no effect on phagocytosis of C. parapsilosis. Consistent with the difference in phagocytosis, neutrophils mediated damage to C. parapsilosis but not C. albicans in assays of residual metabolic activity. C. parapsilosis was more sensitive to oxidative stress, and inclusion of antioxidant in toxicity assays decreased neutrophil mediated damage, suggesting that generation of reactive oxygen species contributes to the mechanism of toxicity. These data suggest that the interaction between neutrophils and Candida species is not uniform and may partially account for differences observed in the epidemiology and natural history of infections caused by these species.

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“…In previous study, the biofilm formation was inhibited with lipase-negative of C. parapsilosis by using lipase inhibitors and their growth was significantly reduced in lipid-rich media. The lipase-negative C. parapsilosis were more efficiently ingested and killed by macrophage-like cells, while less virulent in infections models that involve inoculation of reconstituted human oral epithelium or a murine intraperitoneal challenge (Gácser et al, 2007a). Interestingly, C. parapsilosis infections frequently occur in patients, often LBW neonates, receiving lipid-rich total parenteral nutrition.…”

Section: Discussionmentioning

confidence: 99%

An outbreak of Candida parapsilosis fungemia among preterm infants

Guo

Zhang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. In this article, we describe the first outbreak of Candida parapsilosis fungemia in our hospital. We examined a cluster of four nosocomial cases of C. parapsilosis fungemia that occurred in the neonatal intensive care unit (NICU) of the Affiliated Xingtai People's Hospital of Hebei Medical University over a two-week period. We ascertained patient parameters including clinical characteristics, blood and sputum cultures, and drug sensitivity test results. Cultures from eight blood samples obtained from the four infected preterm infants showed identical characteristics and were identified as C. parapsilosis. In order to determine the infection-related factors and to control the spread of the infection among the population, we immediately initiated the emergency plan. All four of the preterm infants recovered from the infection; there 18260 W. Guo et al.

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Virulence of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis in reconstituted human tissue models

Cited by 118 publications

References 23 publications

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

High efficiency opsonin-independent phagocytosis of Candida parapsilosis by human neutrophils

An outbreak of Candida parapsilosis fungemia among preterm infants

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