Virulence genotyping and population analysis of Streptococcus suis serotype 2 isolates from China

Wang, Dong; Ma, Jiale; Zhu, Yinchu; Zhu, Jielian; Yuan, Lvfeng; Wang, Yanan; Xu, Jiao; Pan, Zihao; Wu, Zongfu; Zhang, Wei; Chen, Lu; Yao, Huochun

doi:10.1016/j.meegid.2015.08.021

Cited by 22 publications

(37 citation statements)

References 34 publications

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“…Among S. suis isolates recovered from diseased ( n = 119) and healthy pigs ( n = 254) (Huang, Shang, Kashif, & Wang, ; Huang et al., ), thirty‐five isolates were unable to be assigned to 29 reference serotypes (Table S2). Twenty‐seven of 35 isolates were identified to Chz or NCLs (NCL1‐20), and the remaining isolates were non‐typeable by PCR assays, suggesting that these isolates carry unidentified NCLs.…”

Section: Resultsmentioning

confidence: 99%

“…Agglutination tests showed that these six isolates were poly‐agglutination or non‐agglutination with the antisera of 29 serotypes and Chz serotype (Table S3). Genomic DNA of these six S. suis isolates was extracted and was submitted for WGS.…”

Section: Resultsmentioning

confidence: 99%

“…To perform the virulence genotyping analysis, the draft genome sequence of all strains was used to detect the presence of 13 virulence‐associated genes of extracellular factor ( epf ), suilysin ( sly ), muramidase‐released protein ( mrp ), fibronectin‐fibrinogen binding protein ( fbps ), peptidoglycan N‐deacetylase ( pgdA ), glutamate dehydrogenase ( gdh ), glyceraldehyde‐3‐phosphate dehydrogenase ( gapdh ), mannose‐specific transport PTS IID ( manN ), phosphoribosylamine‐glycine ligase ( purD ), subtilisin‐like protease ( sspA ), SUM_61‐like ( comR ), permease (SpyM3_0908, scnF ) and endo‐β‐N‐acetylglucosaminidase D ( endoD ) (Dong et al., ; Fittipaldi, Segura, Grenier, & Gottschalk, ). Genes epf , sly , mrp , pgdA , sspA , comR , scnF and endoD that were absent in strains by using draft genome sequences were further validated by PCR assay according to our previous report (Dong et al., ). The primers for those genes are listed in Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…Genes epf , sly , mrp , pgdA , sspA , comR , scnF and endoD that were absent in strains by using draft genome sequences were further validated by PCR assay according to our previous report (Dong et al., ). The primers for those genes are listed in Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…The primers for multiplex PCR assay were designed based on the sequence of gene wzy , namely, cpsJ for NCL21 strain BSJ52, cpsG for NCL22 strain HCJ16, cpsI for NCL23 strain YTJ2, cpsM for NCL24 strain HMJ42, cpsM for NCL25 strain WUSS328 and cpsK for NCL26 strain NJ1112. The primers are listed in Table S1. The PCR mixture contained 1 × Rapid Taq Master Mix (Vazyme, Nanjing, China), 0.1 μM each primer and 50 ng chromosomal DNA in 25 μL final volume.…”

Section: Methodsmentioning

confidence: 99%

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Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Huang

Chen

et al. 2019

Transbound Emerg Dis

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Streptococcus suis is a major swine pathogen and an important zoonotic agent worldwide. At least nine serotypes can infect human so far. Although 29 serotypes (1–19, 21, 23–25, 27–31 and 1/2) strains are considered as authentic S. suis, a novel variant serotype Chz and strains carrying 20 novel capsular polysaccharide loci (NCL) have been identified recently. However, information about pathogenic and antimicrobial resistance characteristics of strains carrying NCLs is still unavailable. In this study, we identified six new NCLs (designated as NCL21‐26) from 35 non‐typeable S. suis strains by agglutination tests and whole genome sequencing analysis. Further analysis of the genetic context of NCL25 and NCL26 showed a mosaic structure of the capsular polysaccharide loci. NCL25 exhibited considerable similarity to that of serotypes 10 and 11, and NCL26 shared similarity to that of serotype 9 and NCL4. Antimicrobial susceptibility testing demonstrated that strains carrying NCL21‐26 were all resistant to clindamycin, lincomycin, erythromycin, tilmicosin and tetracycline. Animal infection experiments showed that the virulence of NCL26 strain NJ1112 isolated from a disease pig was similar to that of S. suis serotype 2 virulent strain SC070731 in both zebrafish and mouse infection models, highlighting the necessity for surveillance of strains belonging to NCL26. We also developed a multiplex PCR assay to detect NCL21‐26 strains. Our findings expand the views of genetic diversity of S. suis capsular polysaccharide loci and S. suis pathogenic characteristic.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Huang

Chen

et al. 2019

Transbound Emerg Dis

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Streptococcus suis in invasive human infections in Poland: clonality and determinants of virulence and antimicrobial resistance

Bojarska

Molska

Janas

et al. 2016

Eur J Clin Microbiol Infect Dis

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The purpose of this study was to perform an analysis of Streptococcus suis human invasive isolates, collected in Poland by the National Reference Centre for Bacterial Meningitis. Isolates obtained from 21 patients during 2000–2013 were investigated by phenotypic tests, multilocus sequence typing (MLST), analysis of the TR9 locus from the multilocus variable number tandem repeat (VNTR) analysis (MLVA) scheme and pulsed-field gel electrophoresis (PFGE) of SmaI-digested DNA. Determinants of virulence and antimicrobial resistance were detected by polymerase chain reaction (PCR) and analysed by sequencing. All isolates represented sequence type 1 (ST1) and were suggested to be serotype 2. PFGE and analysis of the TR9 locus allowed the discrimination of four and 17 types, respectively. Most of the isolates were haemolysis- and DNase-positive, and around half of them formed biofilm. Genes encoding suilysin, extracellular protein factor, fibronectin-binding protein, muramidase-released protein, surface antigen one, enolase, serum opacity factor and pili were ubiquitous in the studied group, while none of the isolates carried sequences characteristic for the 89K pathogenicity island. All isolates were susceptible to penicillin, cefotaxime, imipenem, moxifloxacin, chloramphenicol, rifampicin, gentamicin, linezolid, vancomycin and daptomycin. Five isolates (24 %) were concomitantly non-susceptible to erythromycin, clindamycin and tetracycline, and harboured the tet(O) and erm(B) genes; for one isolate, lsa(E) and lnu(B) were additionally detected. Streptococcus suis isolated in Poland from human invasive infections belongs to a globally distributed clonal complex of this pathogen, enriched in virulence markers. This is the first report of the lsa(E) and lnu(B) resistance genes in S. suis.Electronic supplementary materialThe online version of this article (doi:10.1007/s10096-016-2616-x) contains supplementary material, which is available to authorized users.

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Genomic epidemiology in Streptococcus suis: Moving beyond traditional typing techniques

Hatrongjit,

Fittipaldi,

Gottschalk

et al. 2024

Heliyon

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Virulence genotyping and population analysis of Streptococcus suis serotype 2 isolates from China

Cited by 22 publications

References 34 publications

Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Streptococcus suis in invasive human infections in Poland: clonality and determinants of virulence and antimicrobial resistance

Genomic epidemiology in Streptococcus suis: Moving beyond traditional typing techniques

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