Virulence genotypes, antibiotic resistance and the phylogenetic background of extraintestinal pathogenic Escherichia coli isolated from urinary tract infections of dogs and cats in Brazil

Osugui, Lika; Castro, Antonio Fernando Pestana de; Iovine, R; Irino, Kinue; Carvalho, Vânia Carneiro de

doi:10.1016/j.vetmic.2014.03.027

Cited by 29 publications

(30 citation statements)

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“…The E. coli phylogroup B2 is a possible zoonotic agent that may cross species and cause UTI in humans. 11,15 The B2 phylogroup is highly associated with UTI in humans and this has consistently been reported in the literature. 10,18 However, because not all phylogroup B2 strains are UPEC, 3,13 it is tempting to speculate that B2 UPEC strains are more commonly isolated from “animals that live with humans” because there is an anthropozoonotic association.…”

Section: Discussionsupporting

confidence: 67%

Phylogroup and virulence gene association with clinical characteristics of Escherichia coli urinary tract infections from dogs and cats

et al. 2017

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Escherichia coli isolates from infections outside the gastrointestinal tract are termed extra-intestinal pathogenic E. coli (ExPEC) and can be divided into different subpathotypes; one of these is uropathogenic E. coli (UPEC). The frequency with which UPEC strains cause urinary tract infections in dogs and cats is not well documented. We used an oligonucleotide microarray to characterize 60 E. coli isolates associated with the urinary tract of dogs (n = 45) and cats (n = 15), collected from 2004 to 2007, into ExPEC and UPEC and to correlate results with patient clinical characteristics. Microarray analysis was performed, and phylogroup was determined by a quadruplex PCR assay. Isolates that were missing 1 or 2 of the gene determinants representative of a function (capsule, iron uptake related genes, or specific adhesins) were designated as “non-classifiable” by microarray. Phylogroup B2 was positively associated with the UPEC subpathotype (p < 0.0005) and negatively associated with “non-classifiable” isolates (p < 0.0005). Phylogroup D was positively associated with ExPEC pathotype (p = 0.025) and negatively associated with UPEC subpathotype (p = 0.014). The ExPEC pathotype was positively associated with hospitalization for one or more days (p = 0.031). The UPEC subpathotype was negatively associated with previous antimicrobial therapy (p = 0.045) and previous hospitalization within the 3 mo prior to the positive culture (p = 0.041). The UPEC subpathotype was positively associated with prostatitis (p = 0.073) and negatively associated with current immunosuppressive therapy (p = 0.090). Our results indicate that the case history observations may be critically important during the interpretation of laboratory results to encourage judicious use of antimicrobials.

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Section: Discussionsupporting

confidence: 67%

Phylogroup and virulence gene association with clinical characteristics of Escherichia coli urinary tract infections from dogs and cats

et al. 2017

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“…Interestingly, E. coli strains isolated from septicemic human patients belonged mainly to groups B2 and D (ČUROVÁ et al, 2014). Furthermore, cnf1 strains in phylogroup B2 were associated with diarrhea and mortality of puppies (TURCHETTO et al, 2015) and phylogroup B2 was associated with urine samples that were positive for cnf1 (OSUGUI et al, 2014). Collectively, these results suggested that NTEC from dogs possess ExPEC characteristics.…”

Section: Resultsmentioning

confidence: 84%

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

et al. 2018

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Detecção de genes de virulência e grupos filogenéticos de amostrasEscherichia coli isoladas de cães no Brasil INTRODUCTIONEscherichia coli are a component of normal intestinal microbiota in humans and other animals. Phenotypic and genotypic characteristics allow the identification of E. coli pathogenic strains or pathovars (COURA et al., 2014). Dogs are colonized by E. coli during the first days of their life and some strains can cause enteric or extra-intestinal infections (BEUTIN, 1999 (BEUTIN, 1999). Other pathovars are associated with diarrhea in other animals, namely enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC) (MAINIL, 2013). Because of the close contact with humans, dogs could increase the risk of transmitting potentially zoonotic microorganisms, such as EPEC and STEC (BEUTIN, 1999).In addition to the pathotype and pathovar classification, E. coli strains can be assigned to one of the seven phylogenetic groups, including A, B1, B2, C, D, E, and F (CLERMONT et al., 2013). E. coli genomic structure has shown that the strains belonging to different phylogroups are associated with the disease state and source of isolation (CLERMONT et al., 2013). The extraintestinal pathogenic E. coli (ExPEC) strains are clustered mostly in groups B2 and D and intestinal pathogenic E. coli are mostly in phylogroups A, B1, and E (ESCOBAR- PÁRAMO et al., 2004; CLERMONT et al., 2011). Although phylogenetic characterization is an important tool to improve the understanding of E. coli populations and the relationship between strains and disease (CLERMONT et al., 2011;COURA et al., 2015), few studies have determined phylogenetic groups of E. coli isolated from dogs worldwide, and of those that did, the most frequently used method was a triplex PCR method developed by CLERMONT in 2000. This method is only capable of determining phylogroups A, B1, B2, and D (HARADA et al., 2012;SALVARANI et al., 2012;SCHMIDT et al., 2015) instead of the seven phylogroups that were detected in this study. In Brazil, only two studies identified E. coli pathovars associated with diarrhea in dogs (ALMEIDA et al., 2012;PUÑO-SARMIENTO et al., 2013), but none determined the phylogenetic groups of E. coli. Therefore, this study aimed to detect the virulence genes, pathovars, and phylogenetic groups of E. coli strains isolated from the feces of dogs with and without diarrhea. MATERIALS AND METHODS Stool samplesStool samples were collected from 154 dogs, of which 92 were diarrheic and 62 were without diarrhea. Samples from diarrheic dogs were obtained directly from the rectum, at the Veterinary Hospital of Universidade Federal de Minas Gerais (Belo Horizonte city), upon admission. Collections were performed from dogs that were undergoing a consultation for the occurrence of diarrhea. With owner's consent, fecal samples from non-diarrheic animals were obtained from the dogs of students attending the Universidade Federal de Minas Gerais. For non-diarrheic dogs, fecal samples were either taken directly from the rectum or after spontaneous ...

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“…Henriques et al (2014) also compared E. coli strains isolated from cows with clinical metritis and bitches with pyometra and found that the predominant group was B2 (93%) after group A (7%). In a recent study, Osugui et al (2014) revealed that the most prevalent phylo-group was B2 (62%), D (18%) and B1 (16%) in UTIs of dogs and cats. All these results were obtained from the triplex PCR of Clermont et al (2000), and these confirmed that the most prevalent phylo-group is B2 in E. coli isolated from canine pyometra and also canine UTIs.…”

Section: Resultsmentioning

confidence: 99%

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

Müştak

Günaydin²,

Kaya

et al. 2015

Veterinary Quarterly

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(2015) Phylo-typing of clinical Escherichiacoli isolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR, Veterinary Quarterly, 35:4, 194-199, DOI: 10.1080/01652176.2015 Background: Escherichia coli is one of the major causative agents of bovine mastitis worldwide, and is typically associated with acute, clinical mastitis. Besides this, E. coli strains which belong to the extra-intestinal pathogenic group are also the major cause of urinary tract infections and pyometra in dogs. Objectives: In this study, it was aimed to investigate phylo-groups/subgroups in 155 E. coli isolates obtained from acute bovine mastitis, 43 from urinary tract infections of dogs and 20 from canine pyometra by a formerly described triplex PCR and recently described new quadruplex polymerase chain reaction (PCR) method. Results: Group A 1 (n D 118; 76%) and B1 (n D 71; 46%) were found to be the most prevalent groups by triplex and quadruplex PCR assays in mastitis isolates, respectively. Phylo-typing of 43 urinary tract isolates also revealed that most of the isolates belonged to A 1 (n D 23; 54%) by triplex and B2 (n D 36; 84%) by quadruplex PCR assays. The isolates assigned as group A 1 (n D 17; 85%) by triplex PCR could not be classified by quadruplex PCR in pyometra isolates. Conclusions:The results support the hypothesis that E. coli strains isolated from bovine mastitis cases are environmental. Also, groups C, E and F were identified as new phylo-groups for the first time in acute bovine mastitis cases. The comparison of triplex PCR with quadruplex PCR results revealed that most of the groups assigned in triplex PCR were altered by quadruplex PCR assay.

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Virulence genotypes, antibiotic resistance and the phylogenetic background of extraintestinal pathogenic Escherichia coli isolated from urinary tract infections of dogs and cats in Brazil

Cited by 29 publications

References 24 publications

Phylogroup and virulence gene association with clinical characteristics of Escherichia coli urinary tract infections from dogs and cats

Phylogroup and virulence gene association with clinical characteristics of Escherichia coli urinary tract infections from dogs and cats

Detection of virulence genes and the phylogenetic groups of Escherichia coli isolated from dogs in Brazil

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

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