Virulence Genes, Genetic Diversity, Antimicrobial Susceptibility and Phylogenetic Background of Escherichia coli Isolates

Abdi, Hussein Ali; Ghalehnoo, Mehdi Rashki

doi:10.17795/ijep.25692

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…In the current study, the phylogenetic grouping of tested UPEC revealed that 64.57%, 18.85%, 10.85%, and 5.71% of the isolates belonged to the phylogenetic group B2, D, A, and B1, respectively. Similar phylogenetic grouping studies in Barcelona and Iran showed comparable results, with high predominance of phylogenetic groups B2 and D in UPEC isolates (29,30 (35,36). The current study detected the co-presence of two efflux pump genes, acrA and acrB in 74.28% of tested UPEC clinical isolates, and the presence of tolC in 98.85% of UPEC isolates.…”

Section: Discussionsupporting

confidence: 84%

“…The antibiotic susceptibility of the UPEC isolates was determined by the Kirby-Bauer disk diffusion method, according to the clinical and laboratory standards institute (CLSI) guidelines, using the following antibiotics; amikacin (30 which were supplied by Oxoid (UK). The E. coli strain ATCC 25922 was the reference strain (22).…”

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

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Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance

Gawad¹,

Helmy²,

Tawakkol³

et al. 2018

Jundishapur J Microbiol

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Background: Urinary tract infections are the most commonly encountered infections in clinics and outpatient settings and are mainly caused by Uropathogenic Escherichia coli (UPEC). Multidrug-resistant bacteria (MDR) have become a major public health threat, worldwide. Objectives: This study aimed to investigate the prevalence of the MDR phenotype, efflux pump-mediated resistance, and the ability for in vitro biofilm formation among UPEC clinical isolates from Egypt. Methods: Uropathogenic E. coli isolates were collected from two Egyptian governorates, identified, and classified to their corresponding phylogenetic group by the polymerase chain reaction (PCR). Antimicrobial susceptibility testing was done using the Kirby-Bauer disk diffusion method. AcrAB-TolC efflux pump major genes were detected by PCR; efflux pump-mediated resistance was determined by the efflux pump inhibitor microplate-based assay. The ability for in-vitro biofilm formation was also tested. Results:The phylogenetic analysis of the UPEC isolates revealed that most of the isolates belonged to groups B2 and D. The MDR phenotype was detected in 90.8% of UPEC isolates; efflux pump-mediated resistance was detected in all MDR isolates. The acrA, acrB, and tolC were detected in 74.84% of MDR isolates. The ability for in-vitro biofilm formation was recorded in 76.5% of the UPEC isolates. Conclusions:The MDR phenotype and the ability for in-vitro biofilm formation were predominant among UPEC in Egypt. The high prevalence of MDR efflux pumps necessitates the application of new treatment strategies to inhibit this phenomenon.

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Section: Discussionsupporting

confidence: 84%

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance

Gawad¹,

Helmy²,

Tawakkol³

et al. 2018

Jundishapur J Microbiol

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“…They showed that most extra-intestinal pathogenic strains were in group B2, and then group D. It should be noted that the strains belonging to the phylogenetic group B1 was not found among the studied strains in the present study, being similar with the research of Grude et al (2009) The findings are consistent with the results obtained in the study of Bashir et al (2012) in Faisalabad, Pakistan on 59 UPEC isolates from patients, and one study by Abdi et al (2014) on E. coli isolated from UTIs in Sistan region. These were also consistent with the study of Iranpour et al (2015) who studied the phylogenetic typing of strains of E. coli isolated from UTIs, and with the study conducted by Zhao et al (2009) in China on 202 strains of E. coli isolated from UTIs [12][13][14][15].…”

Section: Discussionsupporting

confidence: 91%

Phylogenetic Classification of Escherichia coli Isolated from Urinary Tract Infections in the Central Regions of Guilan Province

Salehzadeh

Ghauor

2017

JoMMID

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“…Colibacillosis, an infection with Escherichia coli (E. coli) is a major cause of mortality among young calves [1,2]. E. coli is a gram-negative bacterium belonging to the family of Enterobacteriaceae.…”

Section: Introductionmentioning

confidence: 99%

Molecular Study of Colibacillosis Susceptibility in Calves and Lambs

Judi¹,

Judi²,

Saqban³

2020

Nano BioMed ENG

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Single nucleotide polymorphism (SNP) is hereditary change in a DNA sequence that occurs when a single nucleotide in a genome is modified; SNPs are usually considered to be point mutations that have been evolutionarily successful enough to recur in a significant proportion of the population of a species. The ability of certain individuals to respond appropriately to Toll-like receptor (TLR) ligands may be impaired by single-nucleotide polymorphisms (SNPs) within TLR genes, terminating in a modified susceptibility to infectious or inflammatory disease that might contribute to the pathogenesis of complex diseases. Out of the 400 clinical samples of blood collected from calves and lambs suffering from diarrhea, the samples originated from Babylon Veterinary Teaching Hospital and Veterinary Clinics during the period from October 2018 to January 2019, 200 samples were suffering from diarrhea (100 samples of calves and 100 samples of lambs), and 200 samples were the control groups. Genotyping of TLR4 polymorphisms was carried out by using amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique. For calves' TLR4 (rs8193046) gene polymorphisms, the results showed there were significant differences in genotypes and alleles frequencies between the diarrheic cases and control groups (p < 0.05). GG homozygous genotype was overrepresented among diarrheic calves. The frequencies of G allele was higher in diarrheic calves compared to control groups. The calves' GG homozygous genotype was significantly associated with increased susceptibility to Colibacillosis in calves. For lamb-TLR4 (rs160202325) gene polymorphism, AG heterozygous genotype was overrepresented among the cases as of 20 (52.63%), whereas AA was obviously more presented among control individuals as of 6 (60%). There were no difference between diarrheic cases and control groups for allele frequencies.

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Virulence Genes, Genetic Diversity, Antimicrobial Susceptibility and Phylogenetic Background of Escherichia coli Isolates

Cited by 7 publications

References 31 publications

Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance

Antimicrobial Resistance, Biofilm Formation, and Phylogenetic Grouping of Uropathogenic Escherichia coli Isolates in Egypt: The Role of Efflux Pump-Mediated Resistance

Phylogenetic Classification of Escherichia coli Isolated from Urinary Tract Infections in the Central Regions of Guilan Province

Molecular Study of Colibacillosis Susceptibility in Calves and Lambs

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