Virulence Gene’s Relationship With Biofilm Formation and Detection of aac (6’)/aph (2”) in Enterococcus faecalis Isolated From Patients With Urinary Tract Infection

Moniri, Rezvan; Ghasemi, Ahmad; Moosavi, Sayed Mojtaba; Dastehgoli, Kamran; Rezaei, Maryam

doi:10.5812/jjm.6244

Cited by 18 publications

(15 citation statements)

References 28 publications

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“…In our study, the frequency of esp, asa, and cyl genes were 77.8%, 79.4%, and 54% in urine and 74.6%, 65%, and 46% in fecal specimens, respectively. Our result was similar previous reports published by other studies in HA-UTIs [26,[30][31][32] and in opposing with some other reports [25,27].…”

Section: Discussionsupporting

confidence: 87%

Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Ghalavand

Alebouyeh

Ghanati

et al. 2020

Preprint

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Background: Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolated from the urine of symptomatic patients and their fecal samples. Results: Out of the studied patients, 63 pairs of E. faecalis isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤4 µg/ml), while resistance to tetracycline (Urine: 88.9%; stool: 76,2%) and minocycline (Urine: 87.3%, stool: 71.4%) was detected in most of them. The most common detected virulence genes were included efbA, ace, and gelE. RAPD-PCR and PFGE analyses showed the same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively. Conclusions: Similarity of E. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which shows their possible involvement in pathogenicity of uropathogenic E. faecalis strains.

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Section: Discussionsupporting

confidence: 87%

Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Ghalavand

Alebouyeh

Ghanati

et al. 2020

Preprint

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“…In the present study, the prevalence of esp was consistent with those of other studies in Iran and other parts of the world, although some studies failed to find esp + E. faecium (18,36,40,41). The Esp has a role in colonisation and persistence of E. faecalis in the urinary tract (18).…”

Section: Discussionmentioning

confidence: 94%

“…In other studies, asa1 was detected only in E. faecalis strains (28,44,51,54,57). Udo reported a relatively similar frequency of asa1; Moniri and Seno reported a higher frequency (41,58,59).…”

Section: Discussionmentioning

confidence: 99%

“…Investigation of cytolysin genes revealed the presence of cylA, cylB, and cylM in a high proportion of E. faecalis and a low proportion of E. faecium isolates, but in the studies by Udo, Moniri, and Cosentino, the prevalence of cylA, cylB, and cylM were lower than those of the current study (41,59,61). However, Abriouel reported a higher frequency of cyl operon in E. faecalis, although with lower haemolytic activity (62).…”

Section: Discussionmentioning

confidence: 99%

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Virulence Factor and Biofilm Formation in Clinical Enterococcal Isolates of the West of Iran

Azizi¹,

Banafshe²,

Kashef³

et al. 2017

Jundishapur J Microbiol

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Background: Enterococcus spp., a part of the normal flora of the human intestine, possess several virulence factors that can develop biofilms to endure harsh environments. Their ability to cause nosocomial infections makes them as critical opportunistic pathogens in hospital settings. Objectives: The current study aimed at determining the occurrence of 6 genes coding virulence factors and their ability to develop biofilms, and conducting phenotypical assessments of haemolysin and gelatinase in clinical enterococci isolated from the West of Iran. Methods: A total of 126 isolates were screened for harbouring the following genes: aggregation substance (asa1), cytolysin (cylABM), enterococcal surface protein (esp), and gelatinase (gelE). Isolates were tested for haemolysin and gelatinase expression phenotypically and for biofilm production quantitatively, using the microtiter method.Results: Of the 126 tested isolates, 95 (73%) were Enterococcus faecalis and 28 (21%) were E. faecium. The total frequency of virulence gene was cylA 92 (73%), cylB 85 (67%), cylM 57 (45%), asa1 26 (21%), gelE 64 (51%), and esp 66 (53%); while 98 (75%) of the isolates were able to form biofilm. A total of 74 (58%) and 46 (35%) isolates could secret haemolysin and gelatinase. Conclusions: There was a significant difference between the frequency of virulence gene in E. faecalis and E. faecium. Enterococcus faecium isolates lacked the gelE and asa1 genes and the frequency of cylABM genes were lower than that of E. faecalis isolates. Enterococcus faecalis isolates were relatively rich in virulence factors; no association was observed between biofilm formation and the presence of specific virulence genes.

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“…In different studies, the phenotypic and genotypic resistance of Enterococcus and Salmonella isolates against aminoglycosides has been reported from Iran and all over the world (10)(11)(12)(13)(14)(15)(16)(17). This study aimed to investigate the occurrence of aminoglycoside resistance and the prevalence of the resistance-modifying enzyme genes, acc(3)-Ia, aac(6′)-Ib, acc(3)-IIa, armA and rat in Enterococcus and Salmonella strains isolated from two hospitals in Iran (Imam Khomeni , Ilam and Milad, Tehran, Respectively).…”

Section: Objectivesmentioning

confidence: 99%

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Samadi

Pakzad

Sefidan

et al. 2015

Jundishapur J Microbiol

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Background: Aminoglycosides are a group of antibiotics that have been widely used in the treatment of life-threatening infections of Gram-negative bacteria. Objectives: This study aimed to evaluate the frequency of aminoglycoside resistance genes in Enterococcus and Salmonella strains isolated from clinical samples by PCR. Materials and Methods: In this study, 140 and 79 isolates of Enterococcus and Salmonella were collected, respectively. After phenotypic biochemical confirmation, 117 and 77 isolates were identified as Enterococcus and Salmonella, respectively. After the biochemical identification of the isolates, antibiotic susceptibility for screening of resistance was done using the Kirby-Bauer method for gentamicin, amikacin, kanamycin, tobramycin and netilmycin. DNA was extracted from resistant strains and the presence of acc (3)-Ia, aac (3′)-Ib, acc (6)-IIa ,16SrRNA methylase genes (armA and rat) was detected by PCR amplification using special primers and positive controls. Results: Enterococcus isolates have the highest prevalence of resistance to both kanamycin and amikacin (68.4%), and Salmonella isolates have the highest prevalence of resistance against kanamycin (6.9%). Ninety-three and 26 isolates of Enterococcus and Salmonella at least were resistant against one of the aminoglycosides, respectively. Moreover, 72.04%, 66.7%, and 36.6% of the resistant strains of Enterococcus had the aac (3′)-Ia, aac (3′)-IIa, and acc (6′)-Ib genes, respectively. None of the Salmonella isolates have the studied aminoglycoside genes. Conclusions: Our results indicate that acetylation genes have an important role in aminoglycoside resistance of the Enterococcus isolates from clinical samples. Moreover, Salmonella strains indicate very low level of aminoglycoside resistance, and aminoglycoside resistance genes were not found in Salmonella isolates. These results indicate that other resistance mechanisms, including efflux pumps have an important role in aminoglycoside resistance of Salmonella.

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Virulence Gene’s Relationship With Biofilm Formation and Detection of aac (6’)/aph (2”) in Enterococcus faecalis Isolated From Patients With Urinary Tract Infection

Cited by 18 publications

References 28 publications

Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Virulence Factor and Biofilm Formation in Clinical Enterococcal Isolates of the West of Iran

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

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