Virulence factors and antimicrobial resistance of
coagulase-negative staphylococci isolated from
drinking water

Čuvalová, Zuzana; Pipová, M.; Kantíková, M.; Brtková, A.; Fajber, Jozef

doi:10.1515/biol-2015-0034

Cited by 12 publications

(14 citation statements)

References 57 publications

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“…While pathogenicity of S pasteuri is uncertain, it is a known contaminant in human blood of patients that causes bacteremia in humans and found to be resistant to several classes of antibiotics (methicillin/oxacillin, macrolides, lincosamides, streptogramins, tetracyclines, chloramphenicol, streptomycin, fosfomycin, as well as quaternary ammonium compounds (24). The origin of S. pasteuri remains unknown; however, it has been identified in routinely used substances used in human consumption such as fish (25), milk, sausage (26,27) and drinking water (28). Its environmental presence is documented in air samples removed from the stratosphere, at an altitude of 41 km (29).…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus pasteuri isolates (Spvs) from human atherosclerotic plaques mediate virulence, intracellular resistance and transendothelial invasion of macrophages: A mechanistic paradigm for microbial pathogenicity in atherosclerosis

Haque

Modi

Haque

et al. 2019

Preprint

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Atherosclerotic cardiovascular disease, a chronic inflammatory condition of multifactorial etiology, is the leading cause of death worldwide. The concept of 'bacterial persistence' has been proposed as one of several contributing factors to this disease. We hypothesized that the infectious agent(s) found at the site of the atheroma may be dormant but perpetuate virulence in response to host defense and other physiological triggers. In this study, we sought to identify the source of persistent infection in human atherosclerotic plaque and define how pathogen virulence and host defenses mediate plaque vulnerability. Whole genome sequencing (WGS) was used to identify bacteria from pure cultures obtained from atherosclerotic tissues of living subjects diagnosed with more than 70% occlusion of the carotid artery undergoing carotid endarterectomy (CE). WGS identified the predominant species as S. pasteuri (Spv) in all CE isolates grown in pure culture except one isolate which was identified as B. licheniformis. All S. pasteuri isolates (Spvs) were found to contain genes for widespread virulence, invasion, and intracellular resistance. As macrophages (Mφs) play a decisive role at all stages of this disease we treated mouse Mφs (RAW 264.7) with Spvs. While all Spvs tested demonstrated their ability to survive phagocytosis, the highly virulent Spvs also activated Mφs and induced trans-endothelial cell migration of these cells which was mediated via the CC chemokine CCL1 and its receptor CCR8. In conclusion, we show that Spvs are found in CE plaques, have the capacity to survive phagocytosis and induce the transmigration of Mφs across an endothelial barrier in a CCL1-CCR8 dependent process. These findings highlight the significance of carotid vessels as a reservoir for S. pasteuri, a pathogen found in products used routinely in human consumption; this may explain how microbial pathogenicity modulates plaque vulnerability in atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

Staphylococcus pasteuri isolates (Spvs) from human atherosclerotic plaques mediate virulence, intracellular resistance and transendothelial invasion of macrophages: A mechanistic paradigm for microbial pathogenicity in atherosclerosis

Haque

Modi

Haque

et al. 2019

Preprint

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“…These species are reported as less virulent than S. aureus. However, numerous studies have revealed the presence of some virulence factors among CoNS [49][50][51][52], including their ability to produce heat-stable enterotoxins [53,54]. Some CoNS species are capable of causing serious human diseases and nosocomial infections.…”

Section: Clinical Significance Of Staphylococcimentioning

confidence: 99%

“…The increasing clinical significance of CoNS indicates that safety hazards associated with their occurrence in food can be higher than previously assumed [63]. Numerous species of CoNS have been isolated from cheeses, cured meats, sausages, smoked fishes [64], sea-water fish [65], chlorinated drinking water [52], wild rabbits [66], wild pheasants [67] and from Nigerian traditional fermented foods [68]. In general, the occurrence of virulence factors and antimicrobial resistance is more frequent in clinically important CoNS isolates.…”

Section: Clinical Significance Of Staphylococcimentioning

confidence: 99%

“…As compared with isolates from drinking water, virulence factors are more diversified in clinical CoNS strains. Through genes encoding virulence factors or resistance to antibiotics, CoNS may significantly increase the pathogenic potential of the normal skin microflora, including pathogenic coagulase-positive staphylococci [52].…”

Section: Clinical Significance Of Staphylococcimentioning

confidence: 99%

“…The occurrence of staphylococci has been observed in samples of drinking water, wastewaters [235] and biofilms from hospital wastewater [236]. The presence of the mecA gene has been reported in S. epidermidis, present in chlorinated drinking water [52], as well as in a biofilm from hospital wastewaters [236]. Municipal and swine slaughterhouse wastewaters may also participate in the dissemination of the mecA gene and MRSA and pose a health risk not only to workers, but also to the general public [237].…”

Section: Fighting Strategiesmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial resistance in Staphylococci

Pipov¹

2018

Fighting Antimicrobial Resistance

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Characterization and pathogenicity of multidrug-resistant coagulase-negative Staphylococci isolates in chickens

et al. 2023

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The pathogenic potential of vancomycin and methicillin-resistant coagulase-negative Staphylococci (VMRCoNS) on Egyptian poultry farms has received little attention. Therefore, this study aims to study the prevalence of CoNS in imported poultry flocks and commercial poultry farms, evaluate the presence of virulence and antibiotic resistance genes (sea, seb, sec, sed, see, and mecA), and assess their pathogenicity in broiler chicks. Seven species were identified among 25 isolates, such as 8 S. gallinarum, 5 S. saprophyticus, 5 S. chromogens, 3 S. warneri, 2 S. hominis, 1 S. caprae, and 1 S. epidermidis. All isolates were resistant to clindamycin, doxycycline, vancomycin, methicillin, rifampicin, and penicillin. The mecA gene was confirmed in 14 isolates, while the sed gene was revealed in seven isolates. Commercial 1-day-old Ross broiler chicks were divided into eight groups of three replicates (10 birds/group): group Ӏ was negative control; groups (П, Ш, IV, V, VI, VII, and VIII) were subcutaneously inoculated with 108 CFUml−1 of S. hominis, S. caprae, S. epidermidis, S. gallinarum, S. chromogens, S. warneri, and S. saprophyticus, respectively. Groups VIII and V had mortality rates of 100% and 20%, respectively, with no evidence of mortalities in the other groups. The highest re-isolation of CoNS species was recorded in groups VII, VIII, and V. Postmortem and histopathological examination revealed the common presence of polyserositis in the internal organs, and hepatic and myocardial necrosis in groups IV, V, and VI. These findings revealed the pathogenic potential of CoNS, so special attention must be directed toward their public health impact.

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Virulence factors and antimicrobial resistance of coagulase-negative staphylococci isolated from drinking water

Cited by 12 publications

References 57 publications

Staphylococcus pasteuri isolates (Spvs) from human atherosclerotic plaques mediate virulence, intracellular resistance and transendothelial invasion of macrophages: A mechanistic paradigm for microbial pathogenicity in atherosclerosis

Staphylococcus pasteuri isolates (Spvs) from human atherosclerotic plaques mediate virulence, intracellular resistance and transendothelial invasion of macrophages: A mechanistic paradigm for microbial pathogenicity in atherosclerosis

Antimicrobial resistance in Staphylococci

Characterization and pathogenicity of multidrug-resistant coagulase-negative Staphylococci isolates in chickens

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