Virulence-associated gene profiling of Streptococcus suis isolates by PCR

Silva, Luciana M. G.; Baums, Christoph Georg; Rehm, Thomas; Wisselink, Henk J.; Goethe, Ralph; Valentin‐Weigand, Peter

doi:10.1016/j.vetmic.2005.12.013

Cited by 160 publications

(230 citation statements)

References 30 publications

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“…The high frequency of S. suis serotype 2 isolation, especially from the central nervous system, corroborates previous studies of swine infection in Brazil and worldwide (Pagnani et al 2002, Vela et al 2003, Del`Arco et al 2008, Rocha et al 2012. Also the high frequency of mrp + /epf + / sly + and mrp + /epfv/sly + genotypes corroborates previous reports of S. suis infection in Europe and China (Silva et al 2006, Wei et al 2009). …”

Section: Resultssupporting

confidence: 90%

“…The most studied virulence factors are suilysin (SLY; sly), muramidase-released protein (MRP; mrp) and extracellular factor (EF; epf) (Vecht et al 1992, Jacobs et al 1994. Regardless that the mrp + /epf + /sly + genotype is the most prevalent worldwide, it has already been suggested a correlation between the mrp + /epf + /sly + genotype with clinical human and porcine isolates (Silva et al 2006, Wei et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

et al. 2016

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Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp + /epf + /sly + genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

et al. 2016

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“…Three of the 4 are isolates from Canada that belong to ST25 †Isolates with MNCM number and LPH number were isolated from patients at Maharaj Nakorn Chiang Mai Hospital and Lamphun Hospital, Thailand, respectively. ‡Virulence-associated gene profiling was done as described previously (10). cps1J and cps2J, serotype 1 (and 14) and 2 (and 1/2) specific genes, respectively, involved in the capsular biosynthesis; sly, suilysin gene; epf, extracellular factor gene; mrp, muraminidase-released protein gene; +, positive; -, negative.…”

Section: Streptococcus Suis In Humans Thailandmentioning

confidence: 99%

“…A Canadian study reported invasive group A Streptococcus infections following surgery in 1.1 cases per 100,000 admissions (6). Outbreaks have been infrequently described (5,(7)(8)(9)(10), and sources of colonization range from throat to anus and vagina. …”

mentioning

confidence: 99%

in Humans, Thailand

Takamatsu

Wongsawan

Osaki

et al. 2008

Emerg. Infect. Dis.

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“…Necropsies were performed by a standardized protocol and samples for histopathological as well as bacteriological investigations were taken as described previously [46]. Isolated S. suis strains were screened in a MP-PCR for detection of the cps 1 , cps2, cps7, cps9, mrp, epf, sly, arcA and gdh [51]. All isolates from the 10∆ide Ssuis infection group were tested in an ide Ssuis PCR with the primer pair 800vorEcoRI, 800nachEcoRI to confirm the absence of the ide Ssuis gene.…”

Section: Methodsmentioning

confidence: 99%

IgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism

et al. 2018

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Streptococcus suis (S. suis) causes meningitis, arthritis and endocarditis in piglets. The aim of this study was to characterize the IgM degrading enzyme of S. suis (IdeSsuis) and to investigate the role of IgM cleavage in evasion of the classical complement pathway and pathogenesis. Targeted mutagenesis of a cysteine in the putative active center of IdeSsuis abrogated IgM cleavage completely. In contrast to wt rIdeSsuis, point mutated rIdeSsuis_C195S did not reduce complement-mediated hemolysis indicating that complement inhibition by rIdeSsuis depends on the IgM proteolytic activity. A S. suis mutant expressing IdeSsuis_C195S did not reduce IgM labeling, whereas the wt and complemented mutant showed less IgM F(ab’)2 and IgM Fc antigen on the surface. IgM cleavage increased survival of S. suis in porcine blood ex vivo and mediated complement evasion as demonstrated by blood survival and C3 deposition assays including the comparative addition of rIdeSsuis and rIdeSsuis_C195S. However, experimental infection of piglets disclosed no significant differences in virulence between S. suis wt and isogenic mutants without IgM cleavage activity. This work revealed for the first time in vivo labeling of S. suis with IgM in the cerebrospinal fluid of piglets with meningitis. In conclusion, this study classifies IdeSsuis as a cysteine protease and emphasizes the role of IgM cleavage for bacterial survival in porcine blood and complement evasion though IgM cleavage is not crucial for the pathogenesis of serotype 2 meningitis.

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Virulence-associated gene profiling of Streptococcus suis isolates by PCR

Cited by 160 publications

References 30 publications

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

in Humans, Thailand

IgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism

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