2018
DOI: 10.1080/21505594.2018.1496778
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IgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism

Abstract: Streptococcus suis (S. suis) causes meningitis, arthritis and endocarditis in piglets. The aim of this study was to characterize the IgM degrading enzyme of S. suis (IdeSsuis) and to investigate the role of IgM cleavage in evasion of the classical complement pathway and pathogenesis. Targeted mutagenesis of a cysteine in the putative active center of IdeSsuis abrogated IgM cleavage completely. In contrast to wt rIdeSsuis, point mutated rIdeSsuis_C195S did not reduce complement-mediated hemolysis indicating tha… Show more

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Cited by 21 publications
(45 citation statements)
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References 53 publications
(88 reference statements)
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“…(rIde Ssuis _h_95°C) as a negative control as well as the loss-of-function point-mutated variant rIde Ssuis _h_C195S (9). Complete cleavage of IgM in IgG-depleted hyperimmune serum through the addition of rIde Ssuis _h was proven by Western blotting, as demonstrated in Fig.…”
Section: Figmentioning
confidence: 82%
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“…(rIde Ssuis _h_95°C) as a negative control as well as the loss-of-function point-mutated variant rIde Ssuis _h_C195S (9). Complete cleavage of IgM in IgG-depleted hyperimmune serum through the addition of rIde Ssuis _h was proven by Western blotting, as demonstrated in Fig.…”
Section: Figmentioning
confidence: 82%
“…Complement is involved in oxidative burst induction of porcine granulocytes in response to S. suis. To investigate the involvement of complement in the induction of the oxidative burst and killing of streptococci, we used vaccinia virus complement control protein (VCP), which was previously described to be an effective complement inhibitor in porcine blood (9,20). Bactericidal and oxidative burst assays with S. suis strain 10 were conducted in the presence of VCP, apocynin, or the combination of both inhibitors.…”
Section: Figmentioning
confidence: 99%
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“…Copper efflux [20] PmtA Ferrous iron and cobalt efflux, tolerance to hydrogen peroxide-induced oxidative stress [21] MsmK Utilization of multiple carbohydrates, pathogenesis [22,23] Ide Ssuis Degradation of porcine IgM, complement evasion [24][25][26] IgdE Degradation of porcine IgG [27] IgA1 protease/ZmpC Degradation of human IgA1, pathogenesis 1 [28][29][30][31] eSTK Maintaining bacterial morphology, tolerance to stresses, pathogenesis [32][33][34] eSTP Virulence, cell adhesion, and immune evasion 2 [35,36] SspA-1 Virulence, trigger of proinflammatory cytokines [37,38] SspA-2 Pathogenesis, proinflammatory response in macrophages [39][40][41] Superoxide dismutase Oxidative stress resistance, virulence [42,43] NADH oxidase Tolerance to oxidative stress, virulence [44,45] SsnA Degradation of human and porcine neutrophil extracellular traps, pathogenesis [46][47][48] EndAsuis Degradation of neutrophil extracellular traps [49] Enolase Binding of extracellular matrix components, pathogenesis [50][51][52][53][54][55][56][57][58] LuxS Growth, biofilm formation, capsule synthesis, hydrogen peroxide resistance, resistance to fluoroquinolones, pathogenesis [59]…”
Section: Copamentioning
confidence: 99%