Virulence-Associated Gene Profiles of Escherichia coli Isolated from Chickens with Colibacillosis in Japan and Their Correlation with Pathogenicity in Chicken Embryos

Fujimoto, Yoshikazu; Inoue, Hideya; Kanda, Takuya; Ijiri, Moe; Uemura, Ryoko

doi:10.1637/0005-2086-65.3.401

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…However, correlations between the presence of virulence-associated genes and the embryo lethality assay results were not definitive among colibacillosis-related isolates in the present study. Fujimoto et al (2021) reported that several E. coli isolates from broilers with suspected colibacillosis were considered avirulent based on the embryo lethality assay. Our previous study ( Ozaki et al, 2018 ) demonstrated that an avirulent (mortality rate of 17%) E. coli isolate from a layer chicken with colibacillosis caused airsacculitis and pericarditis in experimentally-inoculated chickens.…”

Section: Discussionmentioning

confidence: 99%

Relationship between phylogenetic groups of Escherichia coli and Pathogenicity among Isolates from chickens with Colibacillosis and healthy chickens

Murase¹,

Ozaki²

2022

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Relationship between phylogenetic groups of Escherichia coli and Pathogenicity among Isolates from chickens with Colibacillosis and healthy chickens

Murase¹,

Ozaki²

2022

Poultry Science

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Phenotypic virulence characterization of avian pathogenic Escherichia coli (APEC) isolates from broiler breeders with colibacillosis in Mississippi

Joseph,

Magee,

Jia

et al. 2024

Journal of Applied Microbiology

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Aims In this study, we evaluated the phenotypic virulence characteristics of avian pathogenic Escherichia coli (APEC) isolates from broiler breeders with colibacillosis in Mississippi. Also, the relationship between phenotypic and genotypic virulence patterns was determined. Methods and results Twenty-eight APEC isolated from lesions of broiler breeders diagnosed with colibacillosis were used for embryo lethality assay and chick challenge study. The percentage of embryo mortality following embryo lethality assay and pathogenicity score following the chick challenge study were used to categorize the isolates based on virulence. Pearson correlation analysis was performed to determine the relationship between embryo mortality, chick pathogenicity, and the presence of virulence-associated genes in the isolates. Overall, 39.3% of the isolates were highly virulent and 3.5% were avirulent following both assays. There existed a positive correlation between embryo mortality and chick pathogenicity (r = 0.73, P < 0.01) as well as percentage embryo mortality and pathogenicity score with the presence of some virulence genes. Conclusions Even though all the APEC were isolated from lesions of diseased breeders, the virulence potential varied from being avirulent to highly virulent. Further, we identified a positive relationship between phenotypic virulence and the frequency of virulence-associated genes.

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Antibiotic-Resistant Escherichia coli Strains Isolated from Captive Giant Pandas: A Reservoir of Antibiotic Resistance Genes and Virulence-Associated Genes

Fan

Jiang

Luo

et al. 2022

Veterinary Sciences

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Recent studies showed that Escherichia coli (E. coli) strains isolated from captive giant pandas have serious resistance to antibiotics and carry various antibiotic resistance genes (ARGs). ARGs or virulence-associated genes (VAGs) carried by antibiotic-resistant E. coli are considered as a potential health threat to giant pandas, humans, other animals and the environment. In this study, we screened ARGs and VAGs in 84 antibiotic-resistant E. coli strains isolated from clinically healthy captive giant pandas, identified the association between ARGs and VAGs and analyzed the phylogenetic clustering of E. coli isolates. Our results showed that the most prevalent ARG in E. coli strains isolated from giant pandas is blaTEM (100.00%, 84/84), while the most prevalent VAG is fimC (91.67%, 77/84). There was a significant positive association among 30 pairs of ARGs, of which the strongest was observed for sul1/tetC (OR, 133.33). A significant positive association was demonstrated among 14 pairs of VAGs, and the strongest was observed for fyuA/iroN (OR, 294.40). A positive association was also observed among 45 pairs of ARGs and VAGs, of which the strongest was sul1/eaeA (OR, 23.06). The association of ARGs and mobile gene elements (MGEs) was further analyzed, and the strongest was found for flor and intI1 (OR, 79.86). The result of phylogenetic clustering showed that the most prevalent group was group B2 (67.86%, 57/84), followed by group A (16.67%, 14/84), group D (9.52%, 8/84) and group B1 (5.95%, 5/84). This study implied that antibiotic-resistant E. coli isolated from captive giant pandas is a reservoir of ARGs and VAGs, and significant associations exist among ARGs, VAGs and MGEs. Monitoring ARGs, VAGs and MGEs carried by E. coli from giant pandas is beneficial for controlling the development of antimicrobial resistance.

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Virulence-Associated Gene Profiles of Escherichia coli Isolated from Chickens with Colibacillosis in Japan and Their Correlation with Pathogenicity in Chicken Embryos

Cited by 3 publications

References 21 publications

Relationship between phylogenetic groups of Escherichia coli and Pathogenicity among Isolates from chickens with Colibacillosis and healthy chickens

Relationship between phylogenetic groups of Escherichia coli and Pathogenicity among Isolates from chickens with Colibacillosis and healthy chickens

Phenotypic virulence characterization of avian pathogenic Escherichia coli (APEC) isolates from broiler breeders with colibacillosis in Mississippi

Antibiotic-Resistant Escherichia coli Strains Isolated from Captive Giant Pandas: A Reservoir of Antibiotic Resistance Genes and Virulence-Associated Genes

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