Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken

Abstract

Numerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of poultry E. coli strains in vitro and in vivo via antibiotic susceptibility, biofilm formation and adhesion, and invasion and intracellular survivability assays in Caco-2 and Raw 264.7 cell lines as well as the determination of the median lethal dose in two-day old chickens. A clinical pathogenic multidrug-resistant isolate, E. coli 381, isolated from broilers, was found to be highly virulent in cell culture and 1000-fold more virulent in a chicken model than other strains; accordingly, the isolate was subsequently selected for transcriptome analysis. The comparative gene expression profile of MDR E. coli 381 and the reference human strain E. coli ATCC 25922 was completed with Illumina HiSeq. 2500 transcriptome analysis. Differential gene expression analysis indicates that there are multiple pathways involved in the resistance and virulence of this highly virulent strain. The results garnered from this study provide critical information about the highly virulent MDR E. coli strain of poultry origin and warrant further investigation due to its significant threat to public health.

Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains

“…Most of the multidrug-resistant strains in this study were in group A-UT-␤1. A similar result with group A-MDR in poultry E. coli was reported by another research group (50).…”

Phenotypic and virulence traits ofEscherichia coliandSalmonellastrains isolated from vegetables and fruits from India

“…To the best of our knowledge, we are first to report the invasion/intracellular survival of E. coli and Salmonella strains isolated from the vegetables/fruits. More interestingly, the observed invasive efficiency and intracellular survivability of vegetable-/fruit-origin E. coli and Salmonella isolates were approximately similar to those reported in clinical or animal-isolated strains of E. coli and Salmonella (Pasmans et al 2004;Hussain et al 2017). Considering that invasion/intracellular survivability of pathogens facilitate to evade the host immune system, that allows the establishment of persistent infections (Finlay and Falkow 1997), it can be speculated that the vegetable-/fruit-origin E. coli and Salmonella would pose a potential risk towards public health.…”

“…; Hussain et al . ). Considering that invasion/intracellular survivability of pathogens facilitate to evade the host immune system, that allows the establishment of persistent infections (Finlay and Falkow ), it can be speculated that the vegetable‐/fruit‐origin E. coli and Salmonella would pose a potential risk towards public health.…”

CRISPR-cas3 of Salmonella Upregulates Bacterial Biofilm Formation and Virulence to Host Cells by Targeting Quorum-Sensing Systems

“…The standard growth curves of cas3 WT, ∆cas3, and ∆cas3/p-cas3 strains were determined by a method described previously [60,61]. An overnight culture of three strains grown at 37 • C was inoculated (1:1000) in fresh LB broth, and incubated at 37 • C in a shaker.…”