Virulence and in vitro antifungal susceptibility of Candida albicans and Candida catenulata from laying hens

Rhimi, Wafa; Aneke, Chioma Inyang; Annoscia, Giada; Camarda, Antonio; Cantacessi, Cinzia; Otranto, Domenico; Cafarchia, Claudia

doi:10.1007/s10123-020-00141-1

Cited by 9 publications

(4 citation statements)

References 33 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D. catenulata animal isolates produce and secrete potent hydrolases (e.g., proteases and phospholipases), as previously shown by Brilhante et al [ 19 ] and Rhimi et al [ 43 ]. These enzymes are considered as virulence factors, which play fundamental roles in the pathogenesis of yeast infections [ 44 ].…”

Section: Discussionmentioning

confidence: 57%

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts

Buela

Cuenca

Sarmiento-Ordoñez

et al. 2022

Animals

View full text Add to dashboard Cite

Guinea pigs (Cavia porcellus) have been reared for centuries in the Andean region for ceremonial purposes or as the main ingredient of traditional foods. The animals are kept in close proximity of households and interact closely with humans; this also occurs in western countries, where guinea pigs are considered pets. Even though it is acknowledged that domestic animals carry pathogenic yeasts in their tissues and organs that can cause human diseases, almost nothing is known in the case of guinea pigs. In this work we used traditional microbiological approaches and molecular biology techniques to isolate, identify, and characterize potentially zoonotic yeasts colonizing the nasal duct of guinea pigs raised as livestock in Southern Ecuador (Cañar Province). Our results show that 44% of the 100 animals studied were colonized in their nasal mucosa by at least eleven yeast species, belonging to eight genera: Wickerhamomyces, Diutina, Meyerozyma, Candida, Pichia, Rhodotorula, Galactomyces, and Cryptococcus. Noticeably, several isolates were insensitive toward several antifungal drugs of therapeutic use, including fluconazole, voriconazole, itraconazole, and caspofungin. Together, our results emphasize the threat posed by these potentially zoonotic yeasts to the farmers, their families, the final consumers, and, in general, to public and animal health.

show abstract

Section: Discussionmentioning

confidence: 57%

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts

Buela

Cuenca

Sarmiento-Ordoñez

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…A total of 68 strains isolated from the cloaca of domestic and wild animals or from the skin of hospitalized human patients with M. furfur fungemia were employed for antifungal testing ( Table 5 ). The strains were identified biochemically and molecularly, as previously reported [ 55 ]. All strains were obtained from the fungal collection of the Department of Veterinary Medicine at the University of Bari Aldo Moro, Italy.…”

Section: Methodsmentioning

confidence: 99%

Antifungal, Antioxidant and Antibiofilm Activities of Essential Oils of Cymbopogon spp.

et al. 2022

Self Cite

View full text Add to dashboard Cite

Essential oils (EOs) of Cymbopogon citratus and Cymbopogon proximus are known as sources of monoterpenes and sesquiterpenoids, although their biological activities have not been well investigated. In this study, the compositions of C. citratus and C. proximus EOs of Egyptian origin and their antifungal and antibiofilm properties against Candida spp. and Malassezia furfur were investigated. Antioxidant activities were also evaluated. GC-MS showed the presence of nine and eight constituents in C. citratus and C. proximus EOs, respectively, with geranial and neral as the major compounds of C. citratus EO and piperitone and α-terpinolene as the major compounds of C. proximus EO. Both EOs showed antifungal (MIC values ranging from 1.25 to 20 µL/ mL) and antibiofilm activities (% of reduction ranging from 27.65 ± 11.7 to 96.39 ± 2.8) against all yeast species. The antifungal and antibiofilm activities of C. citratus EO were significantly higher than those observed for C. proximus EO. M. furfur was more susceptible to both EOs than Candida spp. Both EOs exhibited the highest antioxidant activity. This study suggests that C. citratus and C. proximus EOs might be an excellent source of antifungal, antibiofilm and antioxidant drugs and might be useful for preventing Malassezia infections in both medical and veterinary medicine.

show abstract

“…The nuclear ribosomal ITS region was ampli ed using ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') primers (REF). The PCR reaction consisted of 4 µl genomic DNA 100 ng and 46 µl of PCR mix containing 2.5 mM MgCl 2 , 10 mM Tris-HCl (pH8.3) and 50 mM KCl, 250 µM of each dNTP, 100 pmol of each primer and 1.25 U of AmpliTaq Gold (Applied Biosystems, Foster City, CA, USA), and run PCR protocol previously described [9]. PCR products were examined on a 2% agarose gel stained with GelRed (VWR International PBI, Milano, Italy) and visualised on a Gel Logic 100 gel documentation system (Kodak, New York, USA).…”

Section: Molecular Identi Cationmentioning

confidence: 99%

“…For example, C. albicans has been isolated from gut, cloaca and bird droppings of domestic (i.e., laying hens, broiler chickens, pigeons) and wild birds (rhea, psittacinae, raptors, cockatiels) [8]. Besides Candida, other yeast genera such as Cryptococcus, Geotrichum, Rhodotorula and Trichosporon were also isolated from environments, avian sources, and eggs [5,9], with a high number of yeasts species showing azole resistance phenomena, mainly from wild and domestic birds [9,10]. However, it has been shown that the occurrence of different fungal species and their antifungal susceptibility pro les varied accordingly with animal species and geographical area [8,11].…”

Section: Introductionmentioning

confidence: 99%

Wild Boar (Sus scrofa) as Reservoir of Zoonotic Yeasts: Bioindicator of Environmental Quality

et al. 2022

Self Cite

View full text Add to dashboard Cite

Wildlife animals are recognized as reservoirs for zoonotic fungi and their faeces might play an important role in introducing pathogens into the environment. Thought wild boar (Sus scrofa) population has dramatically increased across Europe, information about their possible role in dissemination of zoonotic pathogenic yeasts in the environment is scant. Therefore, fecal samples (n=124) from wild boars from Campania region (Southern Italy) were collected and yeasts identi ed biochemically and molecularly by sequencing of the internal transcribed spacer region (ITS) and their phylogenetical relationship assessed. The antifungal susceptibility pro les of yeasts were also investigated using AFST-EUCAST method. Yeasts were isolated from 50.1% of the samples with the highest occurrence in samples from the province of Salerno (61.1%). A total of 368 Candida strains belonging to nine species were identi ed, with Candida albicans (45.7%), followed by Candida krusei (15.2 %), Candida sloo ae (9.8%) and Candida parapsilosis (7.6%) as the most prevalent identi ed species. Among C. albicans four sequence types (i.e., ST1-ST4) were identi ed with an intraspeci c nucleotide difference up to 0.21%. The ML tree grouped all representative sequence types as paraphyletic clades with those of the references yeast species, respectively and supported by high bootstrap values.Fluconazole was the less active drug whereas, posaconazole, voriconazole, and isavuconazole the most active one. No resistance phenomena were observed for C. albicans and high MICs values for 5FC, azoles and echinocandines were registered in non-albicans Candida spp. This study showed, for the rst time, the important role of wild boars in dissemination of pathogenic fungi in the environment. The absence of resistance phenomena in the Candida spp. might re ect environmental free from residues of azoles antifungals pollution or chemicals and suggests the role of wild boar as bio indicators of environment quality.

show abstract

Virulence and in vitro antifungal susceptibility of Candida albicans and Candida catenulata from laying hens

Cited by 9 publications

References 33 publications

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts

Role of Guinea Pigs (Cavia porcellus) Raised as Livestock in Ecuadorian Andes as Reservoirs of Zoonotic Yeasts

Antifungal, Antioxidant and Antibiofilm Activities of Essential Oils of Cymbopogon spp.

Wild Boar (Sus scrofa) as Reservoir of Zoonotic Yeasts: Bioindicator of Environmental Quality

Contact Info

Product

Resources

About