Virtual Histology of Colorectal Lesions Using Laser-Scanning Confocal Microscopy

Sakashita, Masanori; Inoue, Haruhiro; Kashida, Hiroshi; Tanaka, Junichi; Cho, J. Y.; Satodate, Hitoshi; Hidaka, Eiji; Yoshida, Tomohiko; Fukami, Norio; Tamegai, Yoshiroh; Shiokawa, Akira; Kudo, Shin Ei

doi:10.1055/s-2003-44595

Cited by 91 publications

(73 citation statements)

References 7 publications

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“…The two major features which identified normal and dysplastic or neoplastic tissue were visualisation of the cell nuclei characteristics, and architectural disruption of the mucosal epithelium and vasculature. [55][56][57] The absence of these features appeared to be confirmatory of normal colonic tissue. 55 A new classification for pCLE in the colon has been proposed and showed 'moderate' interobserver agreement for differentiating neoplasia from non-neoplastic tissue.…”

Section: Lower Gastrointestinal Applications Colorectal Cancermentioning

confidence: 75%

“…[55][56][57] The absence of these features appeared to be confirmatory of normal colonic tissue. 55 A new classification for pCLE in the colon has been proposed and showed 'moderate' interobserver agreement for differentiating neoplasia from non-neoplastic tissue. The overall accuracy (81%) for predicting neoplasia was acceptable and became excellent (94%) when all study experts agreed.…”

Section: Lower Gastrointestinal Applications Colorectal Cancermentioning

confidence: 75%

See 1 more Smart Citation

Status of confocal laser endomicroscopy in gastrointestinal disease

Humphris

Swartz

Egan

et al. 2012

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Confocal laser endomicroscopy (CLE) is an advanced imaging technique which combines conventional white light endoscopy (WLE) with an integrated or probe based confocal microscope. This allows microscopic examination of the surface epithelium and in vivo diagnosis during endoscopy. Established CLE applications include the diagnosis of Barrett's oesophagus, gastric intestinal metaplasia, coeliac disease and microscopic colitis. CLE can differentiate hyperplastic from adenomatous polyps in the colon and may obviate the need to biopsy all polyps at endoscopy. CLE is particularly helpful in surveillance endoscopy in inflammatory bowel disease where it has been shown to reduce the number of biopsies required and improve the detection of dysplasia. The future of CLE may be with new contrast agents to allow for molecular tagging and improved endoscopic diagnoses. The aim of this review is to describe the technology and techniques involved in CLE, and discuss the evolving applications in obtaining "virtual biopsy" throughout the GI tract.

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Section: Lower Gastrointestinal Applications Colorectal Cancermentioning

confidence: 75%

Section: Lower Gastrointestinal Applications Colorectal Cancermentioning

confidence: 75%

Status of confocal laser endomicroscopy in gastrointestinal disease

Humphris

Swartz

Egan

et al. 2012

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show abstract

“…can be acquired. The in vivo use of this new imaging system has already been published [23,24] for colonic neoplasms, and the cellular and ductal architecture can be clearly captured. This revolutionary method may enable in vivo real-time pathological diagnosis (virtual biopsy) with the reservation that the lesion is identifi ed beforehand by a conventional method.…”

Section: New Modalities Of Detecting Neoplastic Lesionsmentioning

confidence: 99%

New Diagnostic Approaches for Early Detection of Gastric Cancer

Sugano

Sato

Yao

2004

Dig Dis

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Detection of gastric cancer in early stages is vitally important for ascertaining better prognosis and quality of life for the patients. Therefore, every endoscopist should be trained to master enough diagnostic skills to identify early gastric cancer that often shows minimal alteration from the surrounding mucosa, easily evading detection. For the first step, it is essential that endoscopists understand the normal gastric mucosa as well as the mucosal changes caused by chronic H. pylori infection, a high-risk condition for the development of gastric cancer. Once a suspicious lesion is identified, use of a dye-spraying method may be useful to clarify structural alteration caused by neoplastic changes and facilitate the biopsy sampling. Development of zoom (magnifying) endoscopy enabling 80× magnification with a one-touch switch from conventional endoscopic observation helps to identify the detailed surface structure as well as the vascular architecture of the mucosa without tissue biopsy. Combined with chromoendoscopy, this powerful endoscopic method can be used to identify small cancer foci or delineate the margin of early gastric cancer that can be treatable by mucosal dissection. Other new modalities using a variety of optical devices have been developed but the real value of their utility still remains to be proven in the actual clinical settings.

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“…16 Several ex vivo studies investigated reflectance confocal microscopy in the gastrointestinal tract. [17][18][19][20] Results demonstrated the ability to visualize cellular features in the esophagus, and to detect differences in the nucleus-to-cytoplasm ratio between normal and cancerous tissues. 18 In the colon, hallmark features of normal, hyperplastic, and adenomatous crypts were identified on confocal images.…”

Section: Introductionmentioning

confidence: 99%

“…18 In the colon, hallmark features of normal, hyperplastic, and adenomatous crypts were identified on confocal images. 19 A preliminary report of endoscopic reflectance confocal microscopy has been published, 19 but the method has yet to become widely available in endoscopy.…”

Section: Introductionmentioning

confidence: 99%

Cellular resolution <italic>ex vivo</italic> imaging of gastrointestinal tissues with optical coherence microscopy

et al. 2010

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Abstract. Optical coherence microscopy ͑OCM͒ combines confocal microscopy and optical coherence tomography ͑OCT͒ to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OCM imaging and histology, and to provide a baseline for future endoscopic studies. Co-registered OCM and OCT imaging were performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. Imaging was performed at 1.06-m wavelength with Ͻ2-m transverse and Ͻ4-m axial resolution for OCM, and at 14-m transverse and Ͻ3-m axial resolution for OCT. Multiple sites on 75 tissue samples from 39 patients were imaged. OCM enabled cellular imaging of specimens from the upper and lower gastrointestinal tracts over a smaller field of view compared to OCT. Squamous cells and their nuclei, goblet cells in Barrett's esophagus, gastric pits and colonic crypts, and fine structures in adenocarcinomas were visualized. OCT provided complementary information through assessment of tissue architectural features over a larger field of view. OCM may provide a complementary imaging modality to standard OCT approaches for endoscopic microscopy.

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Virtual Histology of Colorectal Lesions Using Laser-Scanning Confocal Microscopy

Abstract: Laser-scanning confocal microscopy provides immediate images that correspond well with those of hematoxylin-eosin staining. An improved probe-type LCM endomicroscope is being developed which should provide better histological images of colorectal lesions in vivo.

Cited by 91 publications

References 7 publications

Status of confocal laser endomicroscopy in gastrointestinal disease

Status of confocal laser endomicroscopy in gastrointestinal disease

New Diagnostic Approaches for Early Detection of Gastric Cancer

Cellular resolution <italic>ex vivo</italic> imaging of gastrointestinal tissues with optical coherence microscopy

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