Cellular resolution &lt;italic&gt;ex vivo&lt;/italic&gt; imaging of gastrointestinal tissues with optical coherence microscopy

Aguirre, Aaron D.; Chen, Yu; Bryan, Bradley; Mashimo, Hiroshi; Huang, Qin; Connolly, James L.; Fujimoto, James G.

doi:10.1117/1.3322704

Cited by 38 publications

(12 citation statements)

References 42 publications

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“…Optical coherence microscopy (OCM) improves the transverse resolution of OCT by using a high-numerical aperture (NA) objective to generate en face images with cellular-level resolution [3], [4]. Real-time, high-resolution OCM imaging of scattering tissues has been previously performed by raster scanning a tightly focused beam [5]–[7] [see Fig. 1(a)].…”

Section: Introductionmentioning

confidence: 99%

Improved Detection Sensitivity of Line-Scanning Optical Coherence Microscopy

Chen

Huang

Zhou

et al. 2012

IEEE J. Select. Topics Quantum Electron.

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Optical coherence microscopy (OCM) is a promising technology for high-resolution cellular-level imaging in human tissues. Line-scanning OCM is a new form of OCM that utilizes line-field illumination for parallel detection. In this study, we demonstrate improved detection sensitivity by using an achromatic design for line-field generation. This system operates at 830-nm wavelength with 82-nm bandwidth. The measured axial resolution is 3.9 μm in air (corresponding to ~2.9 μm in tissue), and the transverse resolutions are 2.1 μm along the line-field illumination direction and 1.7 μm perpendicular to line illumination direction. The measured sensitivity is 98 dB with 25 line averages, resulting in an imaging speed of ~2 frames/s (516 lines/s). Real-time, cellular-level imaging of scattering tissues is demonstrated using human-colon specimens.

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Section: Introductionmentioning

confidence: 99%

Improved Detection Sensitivity of Line-Scanning Optical Coherence Microscopy

Chen

Huang

Zhou

et al. 2012

IEEE J. Select. Topics Quantum Electron.

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“…A detailed description of the system was previously published. 20,21 Briefly, a femtosecond Nd:glass laser was spectrally broadened to generate an output spectrum centered at 1,060 nm with a greater than 200 nm bandwidth. This enabled an axial resolution of less than 4 μ m for OCT and OCM.…”

Section: Methodsmentioning

confidence: 99%

Integrated Optical Coherence Tomography and Optical Coherence Microscopy Imaging of Ex Vivo Human Renal Tissues

et al. 2012

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Purpose We evaluated the feasibility of using optical coherence tomography and optical coherence microscopy technology to assess human kidney morphology. Materials and Methods A total of 35 renal specimens from 19 patients, consisting of 12 normal tissues and 23 tumors (16 clear cell renal cell carcinomas, 5 papillary renal cell carcinomas and 2 oncocytomas) were imaged ex vivo after surgical resection. Optical coherence tomography and optical coherence microscopy images were compared to corresponding hematoxylin and eosin histology to identify characteristic features of normal and pathological renal tissues. Three pathologists blinded to histology evaluated the sensitivity and specificity of optical coherence microscopy images to differentiate normal from neoplastic renal tissues. Results Optical coherence tomography and optical coherence microscopy images of normal kidney revealed architectural features, including glomeruli, convoluted tubules, collecting tubules and loops of Henle. Each method of imaging renal tumors clearly demonstrated morphological changes and decreased imaging depth. Optical coherence tomography and microscopy features matched well with the corresponding histology. Three observers achieved 88%, 100% and 100% sensitivity, and 100%, 88% and 100% specificity, respectively, when evaluating normal vs neoplastic specimens using optical coherence microscopy images with substantial interobserver agreement (κ = 0.82, p <0.01). Conclusions Integrated optical coherence tomography and optical coherence microscopy imaging provides coregistered, multiscale images of renal pathology in real time without exogenous contrast medium or histological processing. High sensitivity and specificity were achieved using optical coherence microscopy to differentiate normal from neoplastic renal tissues, suggesting possible applications for guiding renal mass biopsy or evaluating surgical margins.

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“…93 In 2010, combined OCT/OCM was used to improve imaging depth and contrast, enabling cellular imaging of human tissues, including those in the upper and lower GI tracts. 94 Coregistered OCM and OCT imaging was performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. 94 OCM enabled cellular imaging of specimens from the upper and lower gastrointestinal tracts over a smaller field of view, compared with OCT. Squamous cells and their nuclei, goblet cells in Barrett's esophagus, gastric pits and colonic crypts, and fine structures in adenocarcinomas were all visualized.…”

Section: Gastrointestinal Cancermentioning

confidence: 99%

“…94 Coregistered OCM and OCT imaging was performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. 94 OCM enabled cellular imaging of specimens from the upper and lower gastrointestinal tracts over a smaller field of view, compared with OCT. Squamous cells and their nuclei, goblet cells in Barrett's esophagus, gastric pits and colonic crypts, and fine structures in adenocarcinomas were all visualized. OCT provided complementary information through the assessment of tissue architectural features over a larger field of view.…”

Section: Gastrointestinal Cancermentioning

confidence: 99%

Review of optical coherence tomography in oncology

2017

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The application of optical coherence tomography (OCT) in the field of oncology has been prospering over the past decade. OCT imaging has been used to image a broad spectrum of malignancies, including those arising in the breast, brain, bladder, the gastrointestinal, respiratory, and reproductive tracts, the skin, and oral cavity, among others. OCT imaging has initially been applied for guiding biopsies, for intraoperatively evaluating tumor margins and lymph nodes, and for the early detection of small lesions that would often not be visible on gross examination, tasks that align well with the clinical emphasis on early detection and intervention. Recently, OCT imaging has been explored for imaging tumor cells and their dynamics, and for the monitoring of tumor responses to treatments. This paper reviews the evolution of OCT technologies for the clinical application of OCT in surgical and noninvasive interventional oncology procedures and concludes with a discussion of the future directions for OCT technologies, with particular emphasis on their applications in oncology.

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Cellular resolution <italic>ex vivo</italic> imaging of gastrointestinal tissues with optical coherence microscopy

Cited by 38 publications

References 42 publications

Improved Detection Sensitivity of Line-Scanning Optical Coherence Microscopy

Improved Detection Sensitivity of Line-Scanning Optical Coherence Microscopy

Integrated Optical Coherence Tomography and Optical Coherence Microscopy Imaging of Ex Vivo Human Renal Tissues

Review of optical coherence tomography in oncology

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