Virological Characteristics of Hospitalized Children With SARS-CoV-2 Infection

Pinninti, Swetha; Pati, Sunil; Poole, Claudette; Latting, Misty; Seleme, Maria C.; Yarbrough, April; Arora, Nitin; Britt, William J.; Boppana, Suresh B.

doi:10.1542/peds.2020-037812

Cited by 19 publications

(18 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 11 , 12 ] However, other reports have reported a good correlation between Ct value and viral loads in samples obtained from children with SARS-CoV-2 infection (r = -1), with lower values of viral loads in asymptomatic children and children aged >1 year. [ 13 ] Some centers are reporting Ct values after normalization or in a semi-quantitative format and, in practice, most centers use CT as a proxy for high or low viral load. Regarding serologic results, because there was appreciable dropout from the 136 included to the 59 who completed the protocol, a selection bias should be considered.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection

Tagarro

Sanz-Santaeufemia

Grasa

et al. 2022

The Journal of Pediatrics

View full text Add to dashboard Cite

Objectives To determine the time to RT-PCR negativity after the first positive RT-PCR test, factors associated with longer time to RT-PCR negativity, proportion of children seroconverting after proven SARS-CoV-2 infection, and factors associated with the lack of seroconversion. Study design EPICO-AEP is a multicenter study conducted in Spanish children to assess the characteristics of COVID-19. In a subset of patients, three serial RT-PCR tests on nasopharyngeal swab specimens were performed after the first RT-PCR test, and IgG serology for SARS-CoV-2 antibodies was performed in the acute and follow up (<14 and ≥14 days after diagnosis) phase. Results In total, 324 patients were included in the study. Median [IQR] time to RT-PCR negativity was 17 [8–29] days, and 35% of patients remained positive >4 weeks after the first RT-PCR test. The probability of RT-PCR negativity did not differ across groups defined by sex, disease severity, immunosuppressive drugs, or clinical phenotype. Globally, 24% of children failed to seroconvert after infection. Seroconversion was associated with hospitalization, persistence of RT-PCR positivity and days of fever. Conclusions Time to RT-PCR negativity was long, regardless of severity of symptoms or other patients’ features. This should be considered when interpreting RT-PCR results in a child with symptoms, especially those with mild symptoms. Seroprevalence and post-immunization studies should consider that one in four infected children fail to seroconvert.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection

Tagarro

Sanz-Santaeufemia

Grasa

et al. 2022

The Journal of Pediatrics

View full text Add to dashboard Cite

show abstract

“…It is important to highlight that it has been previously reported in a study from Latin American where girls had a lower frequency of hospitalization and disease severity than boys. Nevertheless, this report included not only COVID-19 patients but also COVID-19 related Multisystem Inflammatory Syndrome (MIS-C) patients, showing limitations in this report due to diverse clinical courses among both pathologies (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14).…”

Section: Discussionmentioning

confidence: 99%

Correlation of SARS-CoV-2 Viral Load and Clinical Evolution of Pediatric Patients in a General Hospital From Buenos Aires, Argentina

et al. 2022

View full text Add to dashboard Cite

BackgroundSARS-CoV-2 infection is associated with a wide range of clinical manifestations and severity. Pediatric cases represent <10% of total cases, with a mortality rate below 1%. Data of correlation between SARS-CoV-2 viral load in respiratory samples and severity of disease in pediatric patients is scarce. The cycle threshold (CT) value for the detection of SARS-CoV-2 could be used as an indirect indicator of viral load in analyzed respiratory samples.ObjectiveThe aim of this study was to describe CT values and their correlation with clinical manifestations, epidemiology and laboratory parameters in pediatric patients with confirmed COVID-19.MethodsIn this observational, retrospective, analytic and single-center study we included patients under 15 years with confirmed COVID-19 by RT-PCR SARS-CoV-2 admitted to the Isidoro Iriarte Hospital (Argentina) between March 1st 2020 and April 30th 2021.Results485 patients were included, the distribution according to disease severity was: 84% (408 patients) presented mild disease, 12% (59 patients) moderate disease and 4% (18 patients) severe disease. Patients with moderate and severe illness had an increased hospitalization rate, prolonged hospitalization, higher frequency of comorbidities and oxygen and antibiotics use. CT values, that could be used as an indirect measure of viral load, was associated with severity of clinical manifestations and age under 12 months. No patient required admission to PICU nor mechanical ventilation. No deaths were registered.ConclusionsIn this study, the viral load of SARS-CoV-2 in respiratory samples, determined by the cycle threshold, was significantly correlated with moderate to severe cases and with age.

show abstract

“…27 In contrast, some studies show no correlation between age and viral loads. 20 21 22 23 24 25 26 27 28 In addition, male patients' Ct values were lower that suggested a higher viral load. However, gender was not associated with viral load or clinical severity in previous studies.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Alpha Variant (B.1.1.7), Viral Load, and Age on the Clinical Course of Pediatric COVID-19 Patients

Unalan-Altintop

Celep

Milletli-Sezgin

et al. 2022

J Pediatr Infect Dis

View full text Add to dashboard Cite

Objective The research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mainly consists of adult patients, leaving its impact on children understudied. This study aims to investigate the correlations between viral load, clinical course, age, and Alpha variant (B.1.1.7) in children. Methods The study was conducted on children under the age of 18 years, who were admitted to Amasya University Sabuncuoglu Serefeddin Research and Training Hospital in Turkey between February and April 2021. ΔCt values, which were obtained by real-time polymerase chain reaction (PCR), were analyzed to estimate the viral loads of the patients. Alpha variant (B.1.1.7) positivity was determined by real-time PCR. Results There was no difference between estimated viral loads of different clinical courses (p > 0.05), or between asymptomatic and symptomatic patients (p > 0.05). Viral loads were found to decrease with increasing age (p = 0.002). Also, a higher rate of symptomatic disease was found in children under the age of 4 years (p < 0.05). Alpha variant (B.1.1.7) was not found to be associated with severe disease in children (p > 0.05). Conclusion Our results demonstrate higher viral loads and symptomatic disease in children under the age of 4 years. Alpha variant (B.1.1.7) was not found to be related to disease severity. There has not been a consensus on the vaccination of the pediatric population worldwide. More studies are needed to understand the viral kinetics of SARS-CoV-2 and its severity on children to build effective vaccination strategies in children as public health restrictions are eased.

show abstract

Virological Characteristics of Hospitalized Children With SARS-CoV-2 Infection

Cited by 19 publications

References 44 publications

Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection

Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection

Correlation of SARS-CoV-2 Viral Load and Clinical Evolution of Pediatric Patients in a General Hospital From Buenos Aires, Argentina

The Impact of Alpha Variant (B.1.1.7), Viral Load, and Age on the Clinical Course of Pediatric COVID-19 Patients

Contact Info

Product

Resources

About