Virological characteristics of hepatitis B genotype G/A2 recombination virus in Japan

Tsuzuki, Yuji; Watanabe, Tsunamasa; Iio, Etsuko; Fujisaki, Seiichiro; Ibe, Shiro; Kani, Satomi; Hamada-Tsutsumi, Susumu; Yokomaku, Yoshiyuki; Iwatani, Yoshinori; Sugiura, Wataru; Okuse, Chiaki; Okumura, Akihiko; Sato, Yoshihisa; Tanaka, Yasuhito

doi:10.1111/hepr.12612

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…17 The long-term presence of different HBV genotypes in a single patient may increase the risk of recombinant strains with new clinical properties. 32 Herein, the most common recombinant HBV strain was A/G, consistent with other studies. [32][33][34] Unlike elsewhere, we also found recombinant strains with the endemic genotype H, such as H/F and H/G.…”

Section: Discussionsupporting

confidence: 89%

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High Frequency of Antiviral Resistance Mutations in HBV Genotypes A2 and H: Multidrug Resistance Strains in Mexico

José-Ábrego¹,

Román²,

Pinho³

et al. 2023

J Clin Transl Hepatol

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Background and Aims: Lamivudine (3TC), telbivudine (LdT), entecavir (ETV), adefovir (ADF), and tenofovir (TFV) are drugs used to treat hepatitis B virus (HBV) infection, but specific mutations allow some viruses to become resistant to antiviral drugs or to acquire immune escape capacities. These mutations have not been thoroughly investigated in Mexico. This study aimed to estimate the prevalence of HBV antiviral resistance and escape mutations. Methods: This cross-sectional study analyzed 158 samples. HBV DNA was extracted, amplified, and sequenced in serum samples using the spin column method, PCR assay, and Sanger's sequencing, respectively. HBV genotypes were determined, and HBV mutations were tested using the Geno2pheno tool. Results: Overall, 68.4% (108/158) of HBV patients were infected with genotype H, followed by G (11.4%, 18/158), A2 (10.8%, 17/158), F1b (6.9.0%, 11/158), D (1.9%, 3/158), and E (0.6%, 1/158), and 5.1% (8/158) had evidence of recombination. The prevalence of resistance mutations was 8.2% (13/158) and the most common combined mutation was rt180M+rt204V. Notably, we found the combinations rt180M+rt204V+rt173L (n=2) and rt180M+rt204V+rt202G (n=1) that confer multidrug resistance to 3TC, LdT, and ETV. Resistance mutations were found in genotypes A2 (11.8%, 2/17), and H (10.2%, 11/108), and escape mutations were detected in HBV genotypes A2 (11.8%, 2/17), H (10.2%, 11/108), F1b (9.1%, 1/11) and G (5.6%, 1/18). Conclusions: The highest prevalence of antiviral resistance mutations or escape mutations was detected in HBV genotypes A2 and H. The earliest cases of HBV multidrug resistance were detected in Mexico.

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Section: Discussionsupporting

confidence: 89%

“…32 Herein, the most common recombinant HBV strain was A/G, consistent with other studies. [32][33][34] Unlike elsewhere, we also found recombinant strains with the endemic genotype H, such as H/F and H/G.…”

Section: Discussionsupporting

confidence: 89%

High Frequency of Antiviral Resistance Mutations in HBV Genotypes A2 and H: Multidrug Resistance Strains in Mexico

José-Ábrego¹,

Román²,

Pinho³

et al. 2023

J Clin Transl Hepatol

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“…Another study in Japan found HBV/A2 is the most frequent in HIV infected patients and is detected almost exclusively in homosexual patients (Yanagimoto et al 2012 ). Several HBV/G/A2 recombinant strains have been reported among MSM patients in Japan (Tsuzuki et al 2015 ; Kojima et al 2015 ) and all of these strains have a 36-nt insertion of the core gene. However, the insertion was not detected in the HBV/G/A2 recombinant from our patient, suggesting a possibility that he was infected with the strain outside Japan.…”

Section: Discussionmentioning

confidence: 99%

“…Recombination with HIV-HBV/A2 co-infection, which was frequently associated with high HBV-DNA levels, would explain his extremely high HBV-DNA level. Some studies revealed co-infection with HBV/A2 enhanced HBV/G replication (Dao et al 2011 ; Kato et al 2002a , b ; Tanaka et al 2008 ) and a in vivo study demonstrated the introduction of the HBV/A2 core promoter or core protein or both genomic regions into the HBV/G genome increased HBV/G replication efficiently (Tsuzuki et al 2015 ; Sakamoto et al 2013 ). Meanwhile, his CD4+ cell count was maintained at 226/μL, nevertheless HIV-HBV-IRIS occurred after the initiation of ART and HBV-specific CD8+ cell responses could be impaired.…”

Section: Discussionmentioning

confidence: 99%

Human immunodeficiency virus and hepatitis B genotype G/A2 recombinant co-infection: a case study

et al. 2016

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BackgroundHepatitis B virus (HBV) genotypes have distinct geographical distributions and are associated with different clinical courses. HBV genotype G (HBV/G) is extremely rare among Human immunodeficiency virus (HIV) infected populations in Japan. Genetic analysis and clinical course of recombinant forms with HBV/G infection are seldom reported in the literature.Case presentationA 36-year old homosexual man with HIV infection was referred to a general hospital for assessment of chronic HBV infection. We cloned full-length HBV isolates and determined the complete genome sequences of 2 obtained clones, although mixture of multiple variant with different length is detected by HBV-DNA genotyping. The Bootscaning analysis using a full-length HBV genome revealed the clones represented as the HBV/A2 and the HBV/G/A2 recombinant strain. The HBV-DNA decreased from >9.1 to 2.5 log copies/mL after 24 months of antiretroviral therapy.ConclusionsThis patient was co-infected with HBV/A2 and HBV/G/A2 recombinant strain. This recombinant strain was not identical to HBV/G/A2 strains previously reported from Japan. Recombination with other genotypes could alter the clinical manifestations of chronic hepatitis B in people living with HIV.

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Epidemiology and Natural History of Chronic Hepatitis B Virus Infection

Bogler

Wong

Gish

2017

Hepatitis B Virus and Liver Disease

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Virological characteristics of hepatitis B genotype G/A2 recombination virus in Japan

Abstract: We found that four cases of HBV/G/A2 recombinant among MSM patients in the metropolitan areas of Japan, and HBV/A co-infections are required for its efficient replication. High-risk groups such as MSM should be carefully tested for infection of genotype G-derived variants.

Cited by 3 publications

References 33 publications

High Frequency of Antiviral Resistance Mutations in HBV Genotypes A2 and H: Multidrug Resistance Strains in Mexico

High Frequency of Antiviral Resistance Mutations in HBV Genotypes A2 and H: Multidrug Resistance Strains in Mexico

Human immunodeficiency virus and hepatitis B genotype G/A2 recombinant co-infection: a case study

Epidemiology and Natural History of Chronic Hepatitis B Virus Infection

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