“…More recently, advances in flow cytometry instrumentation have allowed for viruses and vesicles in the 100 nm range to readily be detected on high-sensitivity cytometers [ 41 , 42 , 43 , 44 , 45 , 46 ]. With these advances, our lab and others have moved towards staining viral surface antigens on particles directly in cell culture supernatants, without the requirement for magnetic beads or fluorescently tagged labels to detect virions [ 41 , 42 , 43 , 44 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ].…”