Virgin olive oil administration improves the effect of aspirin on retinal vascular pattern in experimental diabetes mellitus

Abstract

The aim of the present study is to evaluate the possible influence of virgin olive oil (VOO) on the effect of acetylsalicylic acid (ASA) in platelet aggregation, prostanoid and NO production and retinal vascular pattern in rats with experimental type 1-like diabetes. We used 100 male Wistar rats that were distributed into five groups: (1) non-diabetic rats (NDR); (2) untreated diabetic rats (DR); (3) DR treated with ASA (2 mg/kg per d per os (p.o.)); (4) DR treated with VOO (0·5 ml/kg per d p.o.); (5) DR treated with ASA plus VOO. The duration of diabetes was 3 months, and each treatment was administered from the first day of diabetes. Variables that were quantified were platelet aggregation (I max ), thromboxane B 2 (TxB 2 ), aortic prostacyclin (6-keto-PGF 1a ) and NO, and the percentage of retina with horseradish peroxidase-permeable vessels (HRP-PV). Diabetic rats showed a higher I max (35 %) and TxB 2 (63 %) than NDR, and a lower 6-keto-PGF 1a , NO and HRP-PV than NDR (2 74·6 %). ASA and VOO administration reduced these differences and prevented the percentage of HRP-PV (2 59·7 % with ASA and 246·7 % with VOO). The administration of ASA plus VOO showed a strong platelet inhibition (80·2 v. 23·4 % for VOO and 50·6 % for ASA þ VOO, P,0·0001), and reduced HRP-PV differences to 2 31·6 % (P,0·001 with respect to DR and P,0·0001 with respect to DR treated with ASA). In conclusion, the administration of VOO to rats with type 1-like diabetes mellitus improves the pharmacodynamic profile of ASA, and increases its retinal anti-ischaemic effect.

Attenuation of streptozotocin-induced microvascular changes in the mouse retina with the endothelin receptor A antagonist atrasentan

“…The lack of change in microvascular density is consistent with our previous results (Wright and Harris 2008) in which we found that capillary filling with FITC-dextran is similar between diabetic and non-diabetic mice at the same time point (4 weeks hyperglycemia) as in the present study. This differs from reports in a different animal model, the diabetic rat (Moreno et al, 1995; De la Cruz et al, 1997), using an assay quantifying capillary density as the number of vessels stained with infused horseradish peroxidase (HRP). In their studies, the number of HRP-stained capillaries decreased significantly within the first four weeks of hyperglycemia in the rat and continued to decrease further in the subsequent weeks.…”

Analgesic, Anti-Inflammatory and Anticancer Activities of Extra Virgin Olive Oil

“…In fact, due to importance of the environment, culture condition, and soil to the final composition of the oil, difference exists between inter/intraregion EVOO compounds. On the other context, due to the benefic effect of the oil, we can propose the EVOO as an adjuvant, vehicle, or emulsion in a treatment context and have a synergistic effect [40] or to prevent undesired effects of other used lipids [41]. …”

Diabetic retinopathy: Role of inflammation and potential therapies for anti-inflammation

“…[61] , is used clinically as a coronary vasodilator and a platelet aggregation inhibitor [62,63] . Dipyridamole plus aspirin improves retinal vasculature patterns in experimental diabetes [64] .…”