Virgibacillus zhanjiangensis sp. nov., a marine bacterium isolated from sea water

Abstract

A Gram-positive, endospore-forming, catalase-and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 079157 T , was isolated from surface seawater off the coastline of Naozhou Island in South China Sea. The organism was able to grow with 1-15% (w/v) total salts (optimum, 4-7%), and at pH 6.0-10.0 (optimum, pH 7.5) and 10-45°C (optimum, 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7, and the polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were anteiso-C 15:0 (45.1%) and anteiso-C 17:0 (16.2%), and the DNA G ? C content was 39.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 079157 T should be assigned to the genus Virgibacillus, being related most closely to the type strains of Virgibacillus litoralis (97.4% sequence similarity), Virgibacillus necropolis (97.3%) and Virgibacillus carmonensis (97.1%). These four strains formed a distinct subcluster in the phylogenetic tree. The levels of DNA-DNA relatedness between the new isolate and the type strains of V. litoralis, V. necropolis and V. carmonensis were 30.4, 19.3 and 12.6%, respectively. The results of the phylogenetic analysis, combined with DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic information, support the suggestion that strain JSM 079157T represents a new species of the genus Virgibacillus, for which the name Virgibacillus zhanjiangensis sp. nov. is proposed. The type strain is JSM 079157 T (=DSM 21084 T = KCTC 13227 T ).Keywords Virgibacillus zhanjiangensis sp. nov. Á Halophilic Á South China SeaThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 079157 T is FJ425904.Electronic supplementary material The online version of this article

Bioflocculant Production by Virgibacillus sp. Rob Isolated from the Bottom Sediment of Algoa Bay in the Eastern Cape, South Africa

“…The genus Virgibacillus are Gram-positive, motile, endospore-forming, irregular rod-shaped and halophilic bacteria [ 19 ], and was first proposed by Heyndrickx et al [ 20 ] and later amended by Heyrman et al [ 21 ]. Now the genus is comprised of sixteen recognized species [ 19 , 22 ].…”

“…The genus Virgibacillus are Gram-positive, motile, endospore-forming, irregular rod-shaped and halophilic bacteria [ 19 ], and was first proposed by Heyndrickx et al [ 20 ] and later amended by Heyrman et al [ 21 ]. Now the genus is comprised of sixteen recognized species [ 19 , 22 ]. The members of the genus are noted for the presence of meso -diaminopimelic acid in their cell-wall peptidoglycan, MK-7 menaquinone, diphosphatidylglycerol and phosphatidylglycerol polar lipids and the G+C content is in the 36–43 mol% range [ 19 , 22 ].…”

“…Now the genus is comprised of sixteen recognized species [ 19 , 22 ]. The members of the genus are noted for the presence of meso -diaminopimelic acid in their cell-wall peptidoglycan, MK-7 menaquinone, diphosphatidylglycerol and phosphatidylglycerol polar lipids and the G+C content is in the 36–43 mol% range [ 19 , 22 ].…”

‘ Gracilibacillus phocaeensis ’ sp. nov., ‘ Sediminibacillus massiliensis ’ sp. nov. and ‘ Virgibacillus ndiopensis ’ sp. nov., three halophilic species isolated from salty human stools by culturomics

“…NR_116658.1) ( Fig. 3 ) [9] . On the basis of this result, we propose to classify ‘ Virgibacillus ndiopensis ’ as a new representative of the Virgibacillus genus belonging to the family Bacillaceae, of the phylum Firmicutes .…”

Virgibacillus halotolerans sp. nov., isolated from a dairy product

“…At the time of writing, the genus Virgibacillus comprises 26 species with validly published names (http://www.bacterio.net/uw/virgibacillus. html); two other species, designated 'Virgibacillus zhanjiangensis' (Peng et al, 2009) and 'Virgibacillus natachei' (Amziane et al, 2013), have been proposed, but these names have not been validly published. Phylogenetically related genera are Oceanobacillus, Ornithinibacillus, Lentibacillus, Paucisalibacillus and Cerasibacillus.…”