Viral vector mimicking and nucleus targeted nanoparticles based on dexamethasone polyethylenimine nanoliposomes: Preparation and evaluation of transfection efficiency

Malaekeh‐Nikouei, Bizhan; Gholami, Leila; Asghari, Fariba; Askarian, Saeedeh; Barzegar, Saeedeh; Rezaee, Mehdi; Oskuee, Reza Kazemi

doi:10.1016/j.colsurfb.2018.02.043

Cited by 11 publications

(10 citation statements)

References 41 publications

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“…For example, liposomes were applied as DNA and RNA nanocarriers 395,396 as well as mimics of the viral envelope for gene delivery. 397 Recent reviews on non-viral application of lipids for gene delivery can be found elsewhere. 398,399 Lipids for viral gene delivery, which are discussed in the following section are summarized in Table 5.…”

Section: Lipidsmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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Section: Lipidsmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

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“… 2016 ; Yoon et al. 2016 ; Malaekeh-Nikouei et al 2018 ). Moreover, with high affinity to its intracellular receptor, Dexa attaches to its cytoplasmic receptor resulting in the transportation of this complex and whatever conjugated to Dexa to the nucleus (Ma et al.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, characterization and evaluation of transfection efficiency of dexamethasone conjugated poly(propyleneimine) nanocarriers for gene delivery#

Malaekeh‐Nikouei

Rezaee

Gholami

et al. 2018

Pharmaceutical Biology

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Context: Polypropylenimine (PPI), a cationic dendrimer with defined structure and positive surface charge, is a potent non-viral vector. Dexamethasone (Dexa) conveys to the nucleus through interaction with its intracellular receptor. Objective: This study develops efficient and non-toxic gene carriers through conjugation of Dexa at various percentages (5, 10 and 20%) to the fourth and the fifth generation PPIs (PPIG4s and PPIG5s). Materials and methods: The 21-OH group of Dexa (0.536 mmol) was modified with methanesulfonyl chloride (0.644 mmol) to activate it (Dexa-mesylate), and then it was conjugated to PPIs using Traut's reagent. After dialysis (48 h) and lyophilization, the physicochemical characteristics of products (PPI-Dexa) including zeta potential, size, buffering capacity and DNA condensing capability were investigated and compared with unmodified PPIs. Moreover, the cytotoxicity and transfection activity of the Dexa-modified PPIs were assessed using Neuro2A cells. Results: Transfection of PPIG4 was close to PEI 25 kDa. Although the addition of Dexa to PPIG4s did not improve their transfection, their cytotoxicity was improved; especially in the carrier to DNA weight ratios (C/P) of one and two. The Dexa conjugation to PPIG5s enhanced their transfection at C/P ratio of one in both 5% (1.3-fold) and 10% (1.6-fold) Dexa grafting, of which the best result was observed in PPIG5-Dexa 10% at C/P ratio of one. Discussion and conclusions: The modification of PPIs with Dexa is a promising approach to improve their cytotoxicity and transfection. The higher optimization of physicochemical characteristics, the better cell transfection and toxicity will be achieved.

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“…Dexamethasone reduced cell proliferation with increasing cell death. As a glucocorticosteroid with immunomodulatory and anti‐inflammation effects, it was expected that dexamethasone adjusted cytotoxicity (Malaekeh‐Nikouei et al, ; Yoon, Yang, Park, Kim, & Kim, ). In this case, dendrimers could not improve the solubility of drug significantly.…”

Section: Discussionmentioning

confidence: 99%

A comparative study on solubility improvement of tetracycline and dexamethasone by poly(propylene imine) and polyamidoamine dendrimers: An insight into cytotoxicity and cell proliferation

Najafi

Salami‐Kalajahi

Roghani‐Mamaqani

et al. 2019

J Biomedical Materials Res

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Many of new chemical discovered in pharmaceutical industry are hydrophobic compounds. Various techniques have been used to overcome solubility problems of hydrophobic drugs in aqueous media. In the meantime, dendrimers have been considered for sustainability, nanoscale size, high carry capacity, tunable terminal functional groups in terms of drug delivery and solubility. In this work, we have synthesized poly(propylene imine) (PPI) dendrimer up to fifth generation using reduction of nitrile groups after Michael addition and also, polyamidoamine (PAMAM) dendrimer up to fourth generation using Michael addition and amidation reactions. fourth and fifth generations of PPI dendrimer and fourth and third generations of PAMAM dendrimer in different concentrations were used to evaluate the solubility of two hydrophobic drugs (tetracycline and dexamethasone). Furthermore, cytotoxicity of dendrimers and dendrimers/drugs hybrids was studied. The results showed that with increasing concentrations and also the generation of dendrimers, the solubility of these two hydrophobic drugs was increased. Cytotoxicity study through MTT assay against Osteoblast-like cell line showed that dendrimers were relatively cytotoxic where adding dexamethasone caused higher cytotoxicity. However, tetracycline showed no significant effect on cytotoxicity whereas prevented cell proliferation. K E Y W O R D Scell proliferation, cytotoxicity, dendrimer, hydrophobic drug, solubility

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Viral vector mimicking and nucleus targeted nanoparticles based on dexamethasone polyethylenimine nanoliposomes: Preparation and evaluation of transfection efficiency

Cited by 11 publications

References 41 publications

Materials promoting viral gene delivery

Materials promoting viral gene delivery

Synthesis, characterization and evaluation of transfection efficiency of dexamethasone conjugated poly(propyleneimine) nanocarriers for gene delivery#

A comparative study on solubility improvement of tetracycline and dexamethasone by poly(propylene imine) and polyamidoamine dendrimers: An insight into cytotoxicity and cell proliferation

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