Viral etiology of community-acquired infection in Vietnam: unraveling the unknown by next-generation sequencing analysis

Nguyen, April; Le, Nikitina; Nguyen, Hoang; Tran, Thi Nhai; Anscombe, Catherine; C-Y., Lau; Limmathurotsakul, Direk; Nguyen, Colleen; Doorn, Rogier van; Deng, Xiaoling; Rahman, Mohammad Saidur; Delwart, Eric; Thwaites, Guy; Le, Thanh Tuan

doi:10.1016/j.ijid.2018.11.285

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“…In Vietnam, there have been some reports of adenoviral conjunctivitis (32,33), and gastroenteritis (13,34,35). HAdV is among the less common viral causes of seasonal respiratory infections as compared to many other more common viruses such as influenza, parainfluenza, respiratory syncytial virus (RSVs), enteroviruses, rhinovirus, etc (36)(37)(38)(39)(40)(41)(42)(43). HAdV is among the co-infection factors in patients with severe pneumonia (39).…”

Section: Introductionmentioning

confidence: 99%

Molecular subtypes of respiratory Adenovirus infection outbreak in children in Northern Vietnam and risk factors of more severe cases

Nguyen¹,

Phung

Huyền³

et al. 2023

Preprint

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Background: Under the pressure of the outbreak of respiratory Human Adenovirus (HAdV) infections in children in Northern Vietnam in the end of 2022, this study was initiated to identify the HAdV subtype(s) responsible for the outbreak in relation to the clinical features of the patients and examine the risk factors of more severe cases. Methods: The study was conducted on pediatric patients tested positive with HAdV using multiplex real-time PCR between October and November 2022. Nasal swab samples were used for sequencing to identify HAdV subtypes and clinical data were collected retrospectively. Results: Among 97 successfully sequenced samples, the predominant subtypes were HAdV-B3 (83%), HAdV-B7 (16%) and HAdV-C2 (1%). Lower respiratory manifestations were found in 25% of patients (5% with severe pneumonia). There was no significant association between HAdV type and clinical features except that those infected with HAdV type 3 exhibited higher WBC and neutrophil % (p<0.001). Co-infection of HAdV with ≥1 other respiratory viruses or bacteria was found in 70.8% of those with lower respiratory illnesses (OR (95%CI); p-value vs. those without =5.21 (1.60, 19.36); 0.0084 after adjusting for age at hospital visit, sex, birth delivery method, day of disease at hospital visit), and in 100% of those with severe pneumonia vs. 33% of those without (p=0.005). Conclusion: HAdV-B3 and HAdV-B7 were predominant in the outbreak. Co-infection of HAdV together with other respiratory viruses or bacteria was a strong risk factor for lower respiratory tract illnesses and severe pneumonia. The findings advocate the advantages of multi-factor microbial panels for the diagnosis and prognosis of respiratory infections in children.

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Section: Introductionmentioning

confidence: 99%

Molecular subtypes of respiratory Adenovirus infection outbreak in children in Northern Vietnam and risk factors of more severe cases

Nguyen¹,

Phung

Huyền³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In Vietnam, there have been some reports of adenoviral conjunctivitis [35,36], and gastroenteritis [12,13,37]. HAdV is among the less common viral causes of seasonal respiratory infections as compared to many other more common viruses such as influenza, parainfluenza, respiratory syncytial virus (RSVs), enteroviruses, and rhinovirus [38][39][40][41][42][43][44][45]. HAdV is among the co-infection factors in patients with severe pneumonia [41].…”

Section: Introductionmentioning

confidence: 99%

Molecular subtypes of Adenovirus-associated acute respiratory infection outbreak in children in Northern Vietnam and risk factors of more severe cases

Nguyen,

Phung,

Thanh Tran

et al. 2023

PLoS Negl Trop Dis

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Background Under the pressure of Human Adenovirus (HAdV)–associated acute respiratory infection (ARI) outbreak in children in Northern Vietnam in the end of 2022, this study was initiated to identify the HAdV subtype(s) and examine the associated clinical features and risk factors of more severe cases. Methods This study evaluated pediatric patients with ARI which had tested positive for HAdV between October and November 2022 using a multiplex real-time PCR panel. Nasopharyngeal aspirates or nasal swab samples were used for sequencing to identify HAdV subtypes. Clinical data were collected retrospectively. Results Among 97 successfully sequenced samples, the predominant subtypes were HAdV-B3 (83%), HAdV-B7 (16%) and HAdV-C2 (1%). Lower respiratory manifestations were found in 25% of the patients of which 5% were diagnosed with severe pneumonia. There was no significant association between HAdV subtype and clinical features except higher white blood cell and neutrophil counts in those detected with HAdV-B3 (p<0.001). Co-detection of HAdV with ≥1 other respiratory viruses was found in 13/24(54%) of those with lower respiratory manifestations and 4/5(80%) of those with severe pneumonia (odds ratio (95% confidence interval) vs. those without = 10.74 (2.83, 48.17) and 19.44 (2.12, 492.73) respectively after adjusting for age, sex, birth delivery method, day of disease). Conclusion HAdV-B3 and HAdV-B7 were predominant in the outbreak. Co-detection of HAdV together with other respiratory viruses was a strong risk factor for lower respiratory tract illnesses and severe pneumonia. The findings advocate the advantages of multi-factor microbial panels for the diagnosis and prognosis of ARI in children.

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Viral etiology of community-acquired infection in Vietnam: unraveling the unknown by next-generation sequencing analysis

Abstract: Abstracts / International Journal of Infectious Diseases 79(S1) (2019) 1-150 115 from malignancy and a higher incidence of comorbidities than the general population without CP.

Cited by 2 publications

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Molecular subtypes of respiratory Adenovirus infection outbreak in children in Northern Vietnam and risk factors of more severe cases

Molecular subtypes of respiratory Adenovirus infection outbreak in children in Northern Vietnam and risk factors of more severe cases

Molecular subtypes of Adenovirus-associated acute respiratory infection outbreak in children in Northern Vietnam and risk factors of more severe cases

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