Aims: Protein S-bacillithiolations are mixed disulfides between protein thiols and the bacillithiol (BSH) redox buffer that occur in response to NaOCl in Bacillus subtilis. We used BSH-specific immunoblots, shotgun liquid chromatography (LC)-tandem mass spectrometry (MS/MS) analysis and redox proteomics to characterize the Sbacillithiolomes of B. subtilis, B. megaterium, B. pumilus, B. amyloliquefaciens, and Staphylococcus carnosus and also measured the BSH/oxidized bacillithiol disulfide (BSSB) redox ratio after NaOCl stress. Results: In total, 54 proteins with characteristic S-bacillithiolation (SSB) sites were identified, including 29 unique proteins and eight proteins conserved in two or more of these bacteria. The methionine synthase MetE is the most abundant Sbacillithiolated protein in Bacillus species after NaOCl exposure. Further, S-bacillithiolated proteins include the translation elongation factor EF-Tu and aminoacyl-tRNA synthetases (ThrS), the DnaK and GrpE chaperones, the two-Cys peroxiredoxin YkuU, the ferredoxin-NADP + oxidoreductase YumC, the inorganic pyrophosphatase PpaC, the inosine-5¢-monophosphate dehydrogenase GuaB, proteins involved in thiamine biosynthesis (ThiG and ThiM), queuosine biosynthesis (QueF), biosynthesis of aromatic amino acids (AroA and AroE), serine (SerA), branched-chain amino acids (YwaA), and homocysteine (LuxS and MetI). The thioredoxin-like proteins, YphP and YtxJ, are S-bacillithiolated at their active sites, suggesting a function in the de-bacillithiolation process. Sbacillithiolation is accompanied by a two-fold increase in the BSSB level and a decrease in the BSH/BSSB redox ratio in B. subtilis. Innovation: Many essential and conserved proteins, including the dominant MetE, were identified in the S-bacillithiolome of different Bacillus species and S. carnosus using shotgun-LC-MS/MS analyses. Conclusion: S-bacillithiolation is a widespread redox control mechanism among Firmicutes bacteria that protects conserved metabolic enzymes and essential proteins against overoxidation.
BackgroundBlood cultures are commonly employed to identify bacterial pathogens causing sepsis. PCR assays to diagnose septicemia require extraction of bacterial DNA from blood samples and thus, delay the initiation of appropriate antimicrobial treatment. The presence of abundant human DNA may hamper the sensitivity of PCR in the detection of bacteria.MethodsWe used serial dilutions of E. Coli spiked pseudo-blood-sepsis samples to develop a simple method that combines the use of a polar detergent solvent and adjustment of the basic pH to remove human DNA. A 16S rRNA gene-based screening algorithm was established to differentiate Gram-positive and Gram-negative groups of bacteria and the family of Enterobacteriaceae. A stringent validation with appropriate controls was implemented. The method of human DNA removal was then applied on 194 sepsis blood samples and 44 cerebrospinal fluid (CSF) samples by real-time PCR.ResultsThis uncomplicated and straightforward approach allows to remove up to 98 % of human DNA from peripheral blood of septic patients. The inhibitory effect of human DNA is efficiently prevented and the detection limit of real-time PCR is increased to 10 E. Coli CFUs/ml. This sensitivity is 10 times higher compared to conventional real-time PCR assays. The classical blood culture detected 58/194 (30 %) of sepsis and 9/44 (21 %) of CSF samples. Out of the 194 blood samples tested, the conventional real-time PCR targeting 13 common sepsis causing pathogens correctly detected the bacterial DNA in 16/194 (8 %) only and 14/44 (32 %) in cerebrospinal fluid samples. Our newly established approach was able to provide correct diagnoses in 78 (40 %) of the 194 blood samples and in 14 (32 %) of the CSF samples. The combination of both blood cultures and our technique raised the rate of sepsis diagnoses to 112/194 (58 %). Of the total group tested positive, 46 (24 %) cases showed overlap with the classical methodology.ConclusionWe report a simple optimized in-house protocol for removal of human DNA from blood sepsis samples as a pre-analytical tool to prepare DNA for subsequent PCR assays. With the detection increase of our in-house DNA removal approach, subsequent PCR assays can reach detection limits of 10 E. coli CFUs/ml and significantly improve the diagnostic rate in blood sepsis cases.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-016-1568-1) contains supplementary material, which is available to authorized users.
Surgical site infection (SSI) is common in Vietnamese post-operative patients. It contributes to increased morbidity, mortality, hospitalization time and health care expenditure. Bacterial culture is considered the gold standard procedure to identify SSI pathogens and antibiotic resistant properties; however, it can detect microbes that can readily grow and is time-consuming. We propose optimized multiplex PCR assays to diagnose the most relevant microbes and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from Vietnamese patients with SSI in a hospital setting in Hanoi.MethodsNinety-one patients (n = 91) were collected in order to identify microbial pathogens and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases by both conventional bacterial culture and in-house multiplex PCR assays.Result and conclusionThe novel in-house multiplex PCR assays are comparable to the bacterial culture approach in screening for common pathogens causing SSI and for relevant genotypes conferring betalactam/carbapenem resistance for bacteria. This is the first report of Turkey-specific ESBL gene (PER-1) and two Oxacilinase families (Oxa23 and Oxa 58) in Vietnam.
BACKGROUND: Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe cutaneous adverse drug reactions. Human leukocyte antigens (HLA) may play an important role in the pathogenesis of SJS/TEN. AIMS: This study aims to identify HLA-B alleles in Vietnamese patients with SJS/TEN and to investigate the possible link between HLA-B alleles and causative drugs. MATERIALS AND METHODS: Sixty patients including SJS (30 patients) and TEN (30 patients) were enrolled in a cross-sectional descriptive study at two hospitals in Hanoi, Vietnam, from July 2018 to July 2019. Clinical features and laboratory findings were noted, HLA-B alleles were analyzed by the polymerase chain reaction (PCR)-sequence-specific oligonucleotide assay and LuminexTM Multiplex Technology. RESULTS: The most common HLA-B allele was HLA-B*15:02 (41.7%) followed by HLA-B*58:01 (25%) and HLA-B*46:01 (15%). Of the 25 patients possessing HLA-B*15:02 allele, culprit medicines were carbamazepine (13 patients; 52%), traditional medicine (two patients; 8%), and unknown drugs (seven patients; 28%). Of the 15 patients carrying HLA-B*58:01 allele, there were 13 patients whose offending medicine was allopurinol. Of the eight patients whose culprit drug was traditional medicine, there were 6 patients (75%) carrying HLA-B*51:02. Patients who carry HLA-B*15:02 were found to have 4 times higher risk of developing carbamazepine-induced SJS/TEN as compared with the tolerant control group (OR=4.17; 95% CI=2.07–8.37; p < 0.001). CONCLUSION: HLA-B*15:02 was the most common HLA-B allele in Vietnamese patients with SJS/TEN. In traditional medicine-induced SJS/TEN patients, HLA-B*51:02 allele might play an important role. The link between the HLA-B genotypes and causative drugs may suggest physicians to avoid risk medications for certain patients.
The effect of three different light qualities on growth, photosynthesis, quality and safe parameters of hydroponic cultivated spinach (Spinacia oleracea L.) were investigated indoor. Three different light qualities were created of red (660 nm), blue (450 nm) and green (550 nm) LEDs corresponding at ratio R660/B450 = 4/1 (RBL); R660/B450/G550= 5/2/3 (WWL); R660/B450/G550 = 1/1/1 (WL), which were tested at the same intensity (PPFD =190 µmol m-2 s-1). The results showed that the plant height and leaf number were the lowest in WL treatment. The SPAD, Net photosynthesis rate Pn, Fv/Fm, Leaf area index LAI values and all parameters of root characteristics were the highest in RBL treatment and were significantly different from two others. Fresh weight of stem, leaf and root, dry weight of root in the three light qualities were significantly different. In contrast, the highest K + content in WL was different from WWL and RBL treatments, while Ca 2+ and Fe 2+ content were the highest in the RBL treatment. Vitamin C content was significantly different between the three treatments. nitrate and oxalic acid contents were the highest in WL treatment, whereas soluble-solids contents and vitamin C contents were the highest in RBL treatment. Oxalic acid, nitrate contents were observed tending reduced under WWL although oxalic acid content in RBL treatment was not different from WL and WWL treatments. In all three different light treatments were not detected Salmonella, E.coli. Our results suggest that RBL may be appropriate light for growth of spinach, but supplementary green light to a combination of red and blue LEDs at the reasonable rate can change the quality of spinach in a positive direction. Hydroponic cultivated spinach was safe for users.
The combination of culture and the multiplex RT-PCR assay provided an excellent diagnostic accomplishment and significantly supported the identification of causative pathogens in clinical samples obtained from septic patients.
Since Jan 4th 2016, the State Bank of Vietnam (SBV) has applied the central exchange rate regime pegging VND to a basket of 8 currencies, which reflects the adaptation of macro policies in general, exchange rate policy in particular when integration context has changed. In order to propose suitable solutions to administrate exchange rate policy effectively, this article employs the VAR model, in which the relationship between exchange rate and three objectives of exchange rate policy (including prices, output and trade balance) are tested. The data used in this model is quarterly, in the period 2001q1-2017q3. Based on the results of the VAR model, a number of policy implications has been proposed, including: (i) continuing to apply currency basket pegged exchange rate regime; (ii) in stead of choosing to devaluate VND, the SBV should use other exchange rate management tools; (iii) speeding up the development of derivative exchange rate market is necessary to reduce the level of ERPT to the import price index so that helps to control inflation in Vietnam and (iv) the SBV should prioritize the exchange rate policy administration towards price stability through adopting the inflation-targeting monetary policy. Keywords Exchange rate policy, exchange rate, inflation, economic growth, trade balance References [1] Campa, J. M. and Goldberg, L. S., “Exchange rate pass-through into import prices”, The Review of Economics and Statistics, 87(4) (2005), pp. 679-690. [2] Ghosh, A. and Rajan, R. S., “Exchange rate pass-through in Korea and Thailand: Trends and determinants”, Japan and the World Economy, No. 21 (2009), pp. 55–70.[3] McCarthy, J., “Pass-Through of Exchange Rates and Import Prices to Domestic Inflation in Some Industrialized Economies”, Eastern Economic Journal, No. 33(4) (2000), pp. 511-537.[4] Hahn, E., Pass-Through of External Shocks to Euro Area Inflation, European Central Bank, Working Paper No.243, 2003.[5] Ito, T. and Sato, K., “Exchange rate changes and inflation in post-crisis Asian economies: VAR analysis of the exchange rate pass-through”, Journal of Money, Credit and Banking, No 40 (2008), pp. 1407-1438.[6] Kim, K. H., “US Inflation and the Dollar Exchange Rate: A Vector Error Correction Model”, Applied Economics, 30(5), 1998, pp.613-619.[7] Beirne, J. and Bijsterbosch, M., Exchange rate pass-through in central and eastern European member states, European Central Bank, Working Paper Series, No.1120, 2009.[8] Huong, T.T.X., V.X.Vinh and N.P. Canh, “Transmission of monetary policy: A number of appropriate regression models”, Journal of Development and Integration, No. 16 (26), 2014, tr.41-46.[9] Vinh, N.T.T, “The role of different channels on trasmitting monetary policy into output and price in Vietnam”, Journal of Economics and Development, No. 214 (2015), tr.20-30.[10] Giang, L.T., Applied structural vector autoregression model to analyze monetary transmission mechanism in Vietnam, Dotoral Thesis, National Economics University, 2017.[11] Trinh, P.T.T., “Impact of foreign exchange reserves to inflation: Approaching by VAR model”, Economic Development Review, No. 26 (2015), tr.46-68.[12] Minh, V.V., Exchange rate pass-through and its implications for inflation in Vietnam, Vietnam development forum, Working paper 0902, 2009.[13] Anh, N.D.M, T.M. Anh and V.T. Thanh, “Exchange rate pass-through into inflation in Vietnam: An assessment using Vector Autogression approach”, Vietnam Economic Management Review, 2010.[14] Anh, P.T., “Applying SVAR model to analyzing exchange rate pass-through effects (ERPT) in Vietnam”, Journal of Economics and Development, No. 220 (2015), tr.48-58. [15] Anh, P.V., Choosing the exchange rate regime in order to implement the inflation targeting policy in Vietnam, Doctoral Thesis, Foreign Trade University, 2017.[16] Minh, H.D., The relationship between inflation and exchange rate in Vietnamese economy, Doctoral Thesis, Hanoi University of Science and Technology, 2014. [17] Hausmann, R., Pritchett, L. and Rodrik, D., Growth accelerations, NBER Working paper series 10566, 2004.[18] Rodrik D., “The Real Exchange Rate and Economic Growth”, Brookings Papers on Economic Activity, Vol. 2008, pp. 365-412. [19] Gluzmann, P. A., Levy – Yeyati, E. and Sturzenegger, F., “Exchange rate undervaluation and economic growth: Díaz Alejandro (1965) revisited”, Economics Letters, No 117 (2012), pp. 666–672.[20] Kappler, M., Reisen, H., Schularick, M. and Turkisch, E., “The Macroeconomic Effects of Large Exchange Rate Appreciations”, Open Econ Rev, No.24 (2012), pp.471–494.[21] Habib, M. M, Mileva, E. and Stracca, L., “The real exchange rate and economic growth: Revisiting the case using external instruments”, Journal of International Money and Finance, Accepted Manuscript, 2017. [22] Rose, A. K., “Exchange rates and the trade balance: Some evidence from developing countries”, Economics Letters, No. 34 (1990), pp.271-275, North-Holland.[23] Vural, B. M. T., “Effect of Real Exchange Rate on Trade Balance: Commodity Level Evidence from Turkish Bilateral Trade Data”, Procedia Economics and Finance, No.38(2016), pp.499 – 507.[24] Trang, L.M, Exchange rate policy to promote export of Vietnam, Doctoral Thesis, Thuong mai University, 2017. [25] Bahmani – Oskooee, M., “Is there a long-run relation between the trade balance and the real effective exchange rate of LDCs?”, Economics Letters, No. 36 (1991), pp.403-407, North-Holland.[26] Anh, D.T.H., Impact of the real exchange rate on trade balace in the context of international economic integration, Doctoral Thesis, Banking Academy, 2012. [27] Arize, A. C., Malindretos, J. and Igwe, E. U., “A Convenient Method for the Estimation of ARDL Parameters and Test statistics: U.S.A Trade Balance and Real Effective Exchange Rate Relation”, International Review of Economics and Finance, 2017, http://dx.doi.org/10.1016/j.iref.2017.03.024.[28] Wang, C. H., Lin, C. H. and Yang. C. H. et al, “Short-run and long-run effects of exchange rate change on trade balance: Evidence from China and its trading partners”, Japan and the World Economy, No. 24 (2012), 266-273.[29] Koray, F. and McMillin, W. D., “Monetary shocks, the exchange rate, and the trade balance”, Journal of International Money and Finance, No.18 (1999), pp.925–940[30] Hang, N.T.T., D.T.Minh, T.T.Thanh, L.H.Giang and P.V.Ha, Exchange rate policy choice in the context of economic recovery, VEPR, Working Paper No, 2010.[31] Nhung, N.C. and T.T.T. Huyen, “Exchange rate pass-through into Vietnamese import prices by industries and by countries”, International Business Management, 11 (11), 2017, pp.1834-1843.
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