Viral etiology of aseptic meningitis among children in Southern Iran

Hosseininasab, Ali; Alborzi, Abdolvahab; Ziyaeyan, Mazyar; Jamalidoust, Marzieh; Moeini, Mahsa; Pouladfar, Gholamreza; Abbasian, Amin; Kadivar, Mohammad Rahim

doi:10.1002/jmv.22056

Cited by 34 publications

(35 citation statements)

References 20 publications

(23 reference statements)

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“…During 2007 -2008 enteroviruses were detected in 30 out of 65 children with viral meningitis in south of Iran (Shiraz city in Fars province) followed by other viruses (46.2%) (15). In another study from south of Iran (Ahvaz city in Khuzestan province) enteroviruses were detected in 52.63% of CSF specimens (16).…”

Section: Discussionmentioning

confidence: 98%

Direct Serotyping of Enteroviruses in Cerebrospinal Fluid of Children With Aseptic Meningitis

Mamishi

Rahimi

Sohrabi

et al. 2013

Jundishapur J Microbiol

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Background: Viral meningitis is an inflammation of the leptomeninges as a manifestation of central nervous system (CNS) infection. More than 85% of viral meningitis cases are caused by non-polio enteroviruses. This is the first study on the description of the enteroviruses and the related serotypes involved in viral meningitis in Iran. Objectives: This project was conducted to improve our knowledge about the role of enteroviruses and their circulating serotypes in viral meningitis in Iran. Patients and Methods: Cerebrospinal fluids from 118 children under 13 years old with primary clinical diagnosis of aseptic meningitis were collected in Children Medical Center in Tehran and sent to Department of Virology of Pasteur Institute of Iran. To investigate the enteroviruses, 5`-noncoding regions were amplified by Real-Time PCR method using Pan-EV primers and a specific probe. Serotype identification in enterovirus positive samples was conducted by RT-PCR. Results: Enterovirus detection rate in all 118 Cerebrospinal fluid specimens was 10.16%. Most patients were 0 -2 years old (40.67%). Serotyping was achieved from 6 specimens with two polio viruses type 1 (vaccine type), two echoviruses 14, one echovirus 5 and one echovirus 30. Conclusions: Enteroviruses should be considered as the main cause of viral meningitis in Iran. Molecular detections of 5-'NCR and VP1-2A RT-PCR with sequence analysis were found to be better than the conventional methods, for direct diagnosis and EVs typing that may lead to decrease of the unnecessary hospitalizations.

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Section: Discussionmentioning

confidence: 98%

Direct Serotyping of Enteroviruses in Cerebrospinal Fluid of Children With Aseptic Meningitis

Mamishi

Rahimi

Sohrabi

et al. 2013

Jundishapur J Microbiol

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“…This result is similar to previous reports (52% and 57%) in Ahvaz, which indicates the predominance of enterovirus infections in patients with aseptic meningitis. [34,35] Hosseininasab et al [36] reported a 43% prevalence of enterovirus infections in patients with aseptic meningitis in Shiraz, Iran. Another study, conducted by Mamishi et al [37] in Tehran, Iran, found that 10.16% of patients with aseptic meningitis have enterovirus infections.…”

Section: Discussionmentioning

confidence: 99%

“…Only one 14-day-old boy, who was born of a mother infected with the varicella-zoster virus at the time of labor, tested positive for VZV infection. Kim et al [39] reported a 14-year-old boy with VZV aseptic meningitis, and Hosseininasab et al [36] reported 2 cases (ages 1–5) of aseptic meningitis with VZV infection. Three cases of mumps virus infection were detected despite the vaccination programme against the mumps virus in Iran.…”

Section: Discussionmentioning

confidence: 99%

Three cases of mumps virus and enterovirus coinfection in children with enteroviral meningitis

Rasti¹,

Makvandi²,

Neisi³

et al. 2016

Medicine

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Several viruses are responsible for aseptic meningitis; however, in the region of Southwest Iran, the role played by each virus is still not very well known. The aim of this study is to determine the relative frequencies of mumps virus, herpes viruses, and enteroviruses, as well as coinfections among them, in patients with aseptic meningitis.In this cross-sectional study, samples of cerebrospinal fluid were collected between December 2012 and December 2013 from patients under 14 years, who were hospitalized in Abuzar Children's Hospital in Ahvaz, Southwest Iran (the only children's hospital in Khuzestan province and Southwest Iran).All 66 cerebrospinal fluid samples and corresponding clinical data were collected from patients with aseptic meningitis by specialists, and with the patients’ consent. The DNA and RNA were extracted from these samples and subjected to polymerase chain reaction as well as reverse transcription polymerase chain reaction (RT-PCR) for detection of mumps virus, herpes viruses, and enteroviruses. Nine of the samples (3 mumps-positive and 6 enterovirus-positive) were sequenced. The mumps virus sequences were investigated for possible mutations in the SH and partial HN regions.Up to 39 patients (59.09%) were found to be positive for enteroviruses, 3 (4.5%) for mumps virus, and 1 (1.5%) for herpes viruses (specifically, the varicella-zoster virus). Two patients (3.03%) had a mumps virus and enterovirus coinfection. Among the 3 detected mumps virus samples, 1 belonged to genotype B, while the others belonged to genotype N. Six sequenced enteroviruses indicated the highest similarity with Echovirus 30. An amino acid substitution at position 51 (N→T) was detected in the HN region of genotype N mumps virus samples, in comparison to the reference strain.

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“…On the other hand, our findings are different from previous reports showing HSV-2 being the most common cause of herpes simplex virus meningitis (5). Recent study in South of Iran revealed the 20% prevalence of HSV meningitis that about 7% belonged to HSV with no discrimination between type 1 and 2 (10). …”

Section: Discussionmentioning

confidence: 99%

Herpes Simplex Virus Meningitis in Children in South East of Caspian Sea, Iran

Azadfar

Cheraghali

Moradi

et al. 2014

Jundishapur J Microbiol

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Background:Herpes simplex virus (HSV) is a member of Herpesviridae and a leading cause of human viral diseases. Meningitis occurs as a complication of HSV-1 or HSV-2 primary infection.Objectives:We aimed to evaluate HSV meningitis in children in Gorgan province, Iran.Patients and Methods:Forty-five cerebrospinal fluid samples were taken from children referred with meningitis symptoms. Samples with negative bacterial culture results were tested for viral, biochemical and cytological assays. DNA extraction and PCR were performed.Results:HSV-1 detected in 4 (8.8%) samples without any HSV-2 infections. Cases with positive results had fever and CSF pleocytosis. Vomiting, headache and higher count of WBC were observed in 3, 2 and 3 cases respectively. The cerebrospinal fluid (CSF) glucose and protein levels were normal and 3 cases showed positive C-reactive protein (CRP) results. Also erythrocyte sedimentation rate (ESR) was higher than normal in all positive cases.Conclusions:Distribution of HSV types in children with meningitis in our area predominantly was type 1 compared with type 2, which has been reported more in other area.

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Viral etiology of aseptic meningitis among children in Southern Iran

Cited by 34 publications

References 20 publications

Direct Serotyping of Enteroviruses in Cerebrospinal Fluid of Children With Aseptic Meningitis

Direct Serotyping of Enteroviruses in Cerebrospinal Fluid of Children With Aseptic Meningitis

Three cases of mumps virus and enterovirus coinfection in children with enteroviral meningitis

Herpes Simplex Virus Meningitis in Children in South East of Caspian Sea, Iran

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