Direct Serotyping of Enteroviruses in Cerebrospinal Fluid of Children With Aseptic Meningitis

Mamishi, Setareh; Rahimi, Pooneh; Sohrabi, Amir; Sabuni, Farah; Edalat, Rosita; Mostafavi, Ehsan; Ashtiani, Mohammad Taghi Haghi; Azadmanesh, Kayhan; Poorakbari, Babak; Motamedi-Rad, Mahdieh; Abdoli, Fatemeh

doi:10.5812/jjm.7852

Cited by 7 publications

(8 citation statements)

References 19 publications

(49 reference statements)

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“…Also the results from repeated cultures and the similar isolation rates in other studies would reject the possibility of contamination of specimens. However, final approval for bacterial meningitis caused by CoNS was accompanied with the results of CSF examination (glucose and protein level) to differentiate from asymptomatic meningitis (22) (16,26). In Ethiopia, N. meningitides was reported as the most common isolate among neonates and young children, and S. pneumonia, as the leading causes of bacterial meningitis in the adults groups, in a one -year hospital survey (17).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Etiological Agents and Antimicrobial Resistance Patterns of Bacterial Meningitis in Nemazee Hospital, Shiraz, Iran

Motamedifar

Ebrahim‐Saraie

Mansury

et al. 2015

Arch Clin Infect Dis

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Background:Meningitis is a serious inflammation of the meninges, mainly caused by bacteria. Treatment of this life threatening disease should not be delayed; therefore, rapid empirical antibiotic therapy should be immediately started. Antimicrobial therapy based on early Cerebrospinal Fluid (CSF) examination has an important role in limitation of bacterial meningitis. Objectives: The current study aimed to identify the prevalence of etiological agents of bacterial meningitis and their susceptibility patterns against commonly used antibiotics. Patients and Methods:This cross-sectional study was performed within a two-year period 2011-2013 on all patients with suspected meningitis referring to Nemazee Hospital, Shiraz, Iran. Cerebrospinal fluid specimens were collected aseptically, and conventional methods were employed to isolate and identify the bacteria. Antimicrobial susceptibility tests were performed using disk diffusion method according clinical and laboratory standards institute (CLSI) recommendation. Results: Out of 2229 suspected meningitis cases, 255 (11.4%) were detected as positive culture. Most of the isolates were Gram-positive cocci, which 116 (45.5%) were Staphylococcus epidermidis, and 20 (7.8%) identified as Streptococcus spp. Among Gram-negative isolates, Escherichia coli and Acinetobacter spp., each with 19 isolates (5.9%), were predominant. Overall, the most effective antibiotics against Gram-positive cocci were vancomycin (91.5%), chloramphenicol (75.2%), and gentamicin (59.3%), respectively. Gram-negative isolates were mostly susceptible to gentamicin (65.1%), chloramphenicol (42.9%), and ciprofloxacin (39.7%), respectively. Conclusions: Gram-positive cocci, such as (Coagulase-Negative Staphylococci) CoNS and aerobic Gram-negative bacilli, were identified as the common etiologic agents of bacterial meningitis. Since the majority of these isolates was part of the normal flora and considered as opportunistic nosocomial infections, it is required to consider the importance of infection control programs in the healthcare centers.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Etiological Agents and Antimicrobial Resistance Patterns of Bacterial Meningitis in Nemazee Hospital, Shiraz, Iran

Motamedifar

Ebrahim‐Saraie

Mansury

et al. 2015

Arch Clin Infect Dis

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“…In contrast to our study, the mean age of the cases in these studies has been mainly less than the 8 years. Another study in children medical center in Tehran showed the "enterovirus detection rate" was 10.16% in all 118 CSFs and most patients were 0 -2 years old (40.67%) (16).…”

Section: Discussionmentioning

confidence: 98%

Molecular Evaluation of Human Enterovirus-Related Aseptic Meningitis in Isfahan, Iran 2017

Chaharsoughi

Ataei

Khorvash

et al. 2020

Jundishapur J Microbiol

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Background: Human enteroviruses (HEVs) cause various infections, especially in children. These viruses are common causes of aseptic meningitis worldwide. Objectives: Limited information about HEV-related aseptic meningitis is available in Isfahan/Iran. Therefore, this study was designed to evaluate HEV-related meningitis in this city. Methods: In this cross-sectional study, 70 patients with the diagnosis of aseptic meningitis were enrolled and divided into 2 groups, including children and adults, in 2 centers of Alzahra and Imam Hossein hospitals from December 2016 to December 2017. Diagnosis of meningitis was made on clinical symptoms and lumbar puncture samples. The RNA of HEV was extracted with Roch extraction Kit and enteroviral infection was detected by real-time PCR test. Chi-square and descriptive statistics were used for data analysis. Results: Enteroviruses were detected in 2 cases out of 70 patients (2.8%). Two cases were in the children group (9.5%). The relationship between gender and prevalence was not significant. Two cases were found in summer and winter. Conclusions: We found that the positive test results of enterovirus in lumbar puncture (LP) samples were significant in the children patients (9.5%, P value = 0.026) but the positive test results in adult patients were zero. So it makes sense to do PCR tests in children with signs or symptoms of meningitis. A well-designed study with more samples is recommended for better evaluation of HEVrelated infections, especially aseptic meningitis in Iran. These types of studies help to find a good strategy for the management of HEV infections in Iran and, thereby reducing hospitalization and overuse of antibiotics.

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“…22,23 It has been shown that PCR is more susceptible than cell culture, especially different clinical samples with low volume and viral load such as cerebrospinal fluid, and some HPeV types are difficult to cultivate. [23][24][25][26][27] In this study, HPeVs were differentiated by RT-PCR amplification method and specific primers from 5′UTR region in viral genome. It has been shown that genotyping of HPeVs is based on VP1 sequence and is related to serotyping.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Respiratory Tract Pathogen Human Parechoviruses in Konya, Turkey

Tokak

Özdemir

Taşbent

2019

J Pediatr Infect Dis

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Objective This study aims to better understand the epidemiological characteristics of parechoviruses and to determine the genotype distribution in Konya, Turkey. Methods In this study, nasal and throat swab samples taken from 1,110 children who were diagnosed with respiratory tract infection (bronchitis, pneumonia, asthmatic bronchitis, and other respiratory diseases) and applied to various pediatric polyclinics of Meram Medical Faculty Hospital, Necmettin Erbakan University between September 2017 and March 2019 were evaluated. Human parechovirus (HPeV) RNA was investigated by multiplex polymerase chain reaction (PCR) in respiratory tract samples. Specific genotypes of the positive samples were identified by real-time PCR amplification of the VP1 region followed by sequence analysis. Results Of the total of 1,110 samples, 4 were positive for HPeV. Of these, HPeV1 was the most predominant genotype (n = 3), followed by HPeV4 (n = 1). HPeV infections were detected throughout the year in Konya, Turkey. Conclusion Although the number of positive samples for HPeV is low, these findings provide information about the genetic diversity and epidemiological of HPeV genotypes circulating in pediatric patients in Turkey. This is the first study to detect prevalence and genotyping of HPeV in respiratory tract infections in Turkey. HPeVs should be considered as causative agents especially in infants with sepsis, meningitis, or encephalitis, and routine testing panels for HPeV detection should be available in hospital laboratories. Further studies using molecular epidemiological methods will be beneficial for identifying genotypes of all HPeVs involved in the etiology and for better monitoring of these infections.

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Direct Serotyping of Enteroviruses in Cerebrospinal Fluid of Children With Aseptic Meningitis

Cited by 7 publications

References 19 publications

Prevalence of Etiological Agents and Antimicrobial Resistance Patterns of Bacterial Meningitis in Nemazee Hospital, Shiraz, Iran

Prevalence of Etiological Agents and Antimicrobial Resistance Patterns of Bacterial Meningitis in Nemazee Hospital, Shiraz, Iran

Molecular Evaluation of Human Enterovirus-Related Aseptic Meningitis in Isfahan, Iran 2017

Investigation of Respiratory Tract Pathogen Human Parechoviruses in Konya, Turkey

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