Serum samples from wild and domestic South American Camelids (SAC) from Argentina, collected before ELISA using a recombinant H1-hemmaglutinin from a reference strain (HA0 PuertoRico/8/1934) was also conducted. Serum samples from Guanacos (126), vicugnas (21) and llamas (100) from Jujuy, Mendoza and Río Negro provinces were analyzed; no clinical signs of respiratory disease were detected, reason for which no nasal swabs were obtained. No seropositive reactors to H3N2 nor H3N8 variants were detected, nevertheless high incidence of Ab reactive to A(H1N1)pdm09 were found by HIA; results which were confirmed by ELISA.The Ab seropositive animals to H1-like IAV found in llamas from Jujuy, and Mendoza (2009) were 78% and 86% by HIA and ELISA, respectively. Thirty-seven samples taken over the three years from guanacos kept in captivity in Rio Negro showed 62% of seropositive animals, while wild guanacos from Mendoza sampled in 2010 showed 36% seropositive animals to H1-like IAV, by both techniques. Finally, wild vicugnas from Jujuy,
Material and Methods
Serum SamplesSerum samples (n = 247) from three SAC species living Then, sera were hemoadsorbed with 10 ul of guinea pig/
Statistical MethodsSerum samples with a HIA Ab titers > 1:40 were considered positive -i. e. evidence of infection with IAV.Serum samples with ELISA Ab titers to H1 HA0 ≥ 1:256were considered as positive sample using previous data and control samples [27].
ResultsDuring this study, blood samples from 247 SAC (Table 1) ence is marked using letters, same letter show no differences (KW, p<0.05, Tukey post hoc test). As positive control llama hyperimmunized with a Human Flu vaccines was included and plotted plotted in last place of serial data.