Viral detection profile in children with severe acute respiratory infection

Canela, Luciana Nascimento Pinto; Magalhães‐Barbosa, Maria Clara de; Raymundo, Carlos Eduardo; Carney, Sharon; Siqueira, Marilda Mendonça; Prata‐Barbosa, Arnaldo; Cunha, Antônio José Lêdo Alves da

doi:10.1016/j.bjid.2018.09.001

Cited by 16 publications

(15 citation statements)

References 32 publications

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“…e IV is known for its seasonal outbreaks of ARI during a few weeks in winter [29]. According to a study realized in Brazil by Canela et al, among children aged between 0 and 18 years, the virus positivity is higher with frequency of IV H1N1 (38%) [32]. PIV subtype 4 was detected in 2% of our cases, and it has been reported to be a much less frequent cause of ARIs [33].…”

Section: Discussionmentioning

confidence: 47%

Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants

Hattoufi

Tligui²,

Obtel

et al. 2020

Advances in Virology

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Background. In Morocco, pediatric pneumonia remains a serious public health problem, as it constitutes the first cause of mortality due to infectious diseases. e etiological diagnosis of acute respiratory tract infections is difficult. erefore, it is necessary to use Multiplex real-time polymerase chain reaction assay tests in a routine setting for exact and fast identification. Objectives. In this paper, we present the clinical results of pediatric pneumonia and describe their etiology by using molecular diagnosis. Study design: Tracheal secretion was collected from infants presenting respiratory distress isolated or associated with systemic signs, attending the unit of Neonatology between December 1, 2016, and Mai 31, 2018. Samples were tested with the multiplex RespiFinder ® SMART 22 FAST which potentially detects 18 viruses and 4 bacteria. Results. Of the 86 infants considered in this study (mean age 31 ± 19 days) suspected of acute respiratory tract infections, 71 (83%) were positive for one or multiple viruses or/and bacteria. e majority of acute respiratory tract infections had a viral origin (95%): respiratory syncytial viruses (A and B) (49%), rhinovirus (21%), coronaviruses 229E (11%), humain metapneumovirus (5%), influenza A (3%), influenza H1N1 (1%), adenovirus (2%), and parainfluenza virus type 4 (2%). Among our patients, 6% had Mycoplasma pneumoniae. Coinfections were not associated with severe respiratory symptoms. Conclusion. e clinical spectrum of respiratory infections is complex and often nonspecific. us, the early and fast detection of related causative agents is crucial. e use of multiplex real time polymerase chain reaction may help choose an accurate treatment, reduce the overall use of unnecessary antibiotics, preserve intestinal flora, and decrease nosocomial infection by reducing the length of hospitalization.

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Section: Discussionmentioning

confidence: 47%

Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants

Hattoufi

Tligui²,

Obtel

et al. 2020

Advances in Virology

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“…Co-detection with other viruses was observed in 15% of the patients in which it was investigated (only in non-MIS-C group), which is similar to other COVID-19 studies, 5 , 10 and in other severe respiratory virus infections in children. 17 …”

Section: Discussionmentioning

confidence: 99%

Pediatric patients with COVID-19 admitted to intensive care units in Brazil: a prospective multicenter study

Prata‐Barbosa

Lima‐Setta

Santos

et al. 2020

Jornal de Pediatria

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Objective To describe the clinical characteristics of children and adolescents admitted to intensive care with confirmed COVID-19. Method Prospective, multicenter, observational study, in 19 pediatric intensive care units. Patients aged 1 month to 19 years admitted consecutively (March–May 2020) were included. Demographic, clinical-epidemiological features, treatment, and outcomes were collected. Subgroups were compared according to comorbidities, age < 1 year, and need for invasive mechanical ventilation. A multivariable logistic regression model was used for predictors of severity. Results Seventy-nine patients were included (ten with multisystemic inflammatory syndrome). Median age 4 years; 54% male (multisystemic inflammatory syndrome, 80%); 41% had comorbidities (multisystemic inflammatory syndrome, 20%). Fever (76%), cough (51%), and tachypnea (50%) were common in both groups. Severe symptoms, gastrointestinal symptoms, and higher inflammatory markers were more frequent in multisystemic inflammatory syndrome. Interstitial lung infiltrates were common in both groups, but pleural effusion was more prevalent in the multisystemic inflammatory syndrome group (43% vs. 14%). Invasive mechanical ventilation was used in 18% (median 7.5 days); antibiotics, oseltamivir, and corticosteroids were used in 76%, 43%, and 23%, respectively, but not hydroxychloroquine. The median pediatric intensive care unit length-of-stay was five days; there were two deaths (3%) in the non- multisystemic inflammatory syndrome group. Patients with comorbidities were older and comorbidities were independently associated with the need for invasive mechanical ventilation (OR 5.5; 95% CI, 1.43–21.12; p = 0.01). Conclusions In Brazilian pediatric intensive care units, COVID-19 had low mortality, age less than 1 year was not associated with a worse prognosis, and patients with multisystemic inflammatory syndrome had more severe symptoms, higher inflammatory biomarkers, and a greater predominance of males, but only comorbidities and chronic diseases were independent predictors of severity.

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“…Although single respiratory viruses can be detected in patients with symptoms, other respiratory viruses may also exist simultaneously. Children, especially those under 5 years of age, present with a higher frequency of coinfections 70–72 . Multiplex assays that contain more than one viral gene target in a single tube have the advantage of rapid detection of several potential viral pathogens simultaneously.…”

Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

Recent advances in the detection of respiratory virus infection in humans

Zhang

Wang

Deng

et al. 2020

Journal of Medical Virology

427

363

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Respiratory tract viral infection caused by viruses or bacteria isone of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019-nCoV that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray-based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections. K E Y W O R D S adenovirus, coronavirus, diagnostic methods, influenza virus, respiratory syncytial virus, respiratory viral infection, rhinovirus

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Viral detection profile in children with severe acute respiratory infection

Cited by 16 publications

References 32 publications

Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants

Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants

Pediatric patients with COVID-19 admitted to intensive care units in Brazil: a prospective multicenter study

Recent advances in the detection of respiratory virus infection in humans

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