Viral detection by electron microscopy: past, present and future

Roingeard, Philippe

doi:10.1042/bc20070173

Cited by 107 publications

(73 citation statements)

References 92 publications

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“…Sapoviruses are morphologically distinguishable from other gastroenteritis pathogens (e.g., norovirus, rotavirus, astrovirus, or adenovirus) by their typical "Star of David" surface morphology under the electron microscope (1,33,90,125) (Fig. 1).…”

Section: Laboratory Diagnosis Virus Particle Detectionmentioning

confidence: 99%

Comprehensive Review of Human Sapoviruses

et al. 2015

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SUMMARY Sapoviruses cause acute gastroenteritis in humans and animals. They belong to the genus Sapovirus within the family Caliciviridae . They infect and cause disease in humans of all ages, in both sporadic cases and outbreaks. The clinical symptoms of sapovirus gastroenteritis are indistinguishable from those caused by noroviruses, so laboratory diagnosis is essential to identify the pathogen. Sapoviruses are highly diverse genetically and antigenically. Currently, reverse transcription-PCR (RT-PCR) assays are widely used for sapovirus detection from clinical specimens due to their high sensitivity and broad reactivity as well as the lack of sensitive assays for antigen detection or cell culture systems for the detection of infectious viruses. Sapoviruses were first discovered in 1976 by electron microscopy in diarrheic samples of humans. To date, sapoviruses have also been detected from several animals: pigs, mink, dogs, sea lions, and bats. In this review, we focus on genomic and antigenic features, molecular typing/classification, detection methods, and clinical and epidemiological profiles of human sapoviruses.

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Section: Laboratory Diagnosis Virus Particle Detectionmentioning

confidence: 99%

Comprehensive Review of Human Sapoviruses

et al. 2015

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“…The electron microscopy has been very helpful to identify several viral agents during outbreaks (Roingeard, 2008) being elected as an important tool in the study of viruses with icosahedral structure (Garcea & Cahen, 2007).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

et al. 2013

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SUMMARY:Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin lesion fragments. The ultrathin sections revealed the presence of groups of viral, intranuclear, rounded particles measuring 35 nm in diameter and tubular particles with a diameter of 35-39 nm. At immunoelectron microscopy technique, positivity obtained was marked by the presence of aggregates of viral particles formed by the antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction showed colloidal gold particles evenly distributed over the surface of the virus. These results showed the importance of the transmission electron microscopy techniques in the diagnosis of bovine papillomatosis that can be used in routine procedures to identify viral agent of this important disease.

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“…The transmission electron microscopy technique has been useful for identification of unknown viruses, enabling rapid identification of these agents (Gentile& Gelderblom, 2005;Roingeard, 2008).…”

Section: Introductionmentioning

confidence: 99%

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Catroxo¹,

Martins²,

Petrella³

et al. 2012

Int. J. Morphol.

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SUMMARY:Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered with spikes and characteristic helical herring-bone-like nucleocapsid measuring 15 to 20 nm in diameter. Ultrathin sections of the small intestine fragments revealed the presence of amorphous granular intracytoplasmic inclusions surrounded by membrane and containing viral nucleocapsid measuring 10-14 nm in diameter. Immature particles budding from cell membranes, pleomorphic, spherical and tubular particles containing viral nucleocapsid strands, and the complete particles measured up to 170 nm in diameter were seen in the cytoplasm. Intranuclear inclusions containing viral nucleocapsid were also visualized. Nuclei showed a marginalized chromatin.

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Viral detection by electron microscopy: past, present and future

Cited by 107 publications

References 92 publications

Comprehensive Review of Human Sapoviruses

Comprehensive Review of Human Sapoviruses

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

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