Vimentin and laminin are altered on cheek pouch microvessels of streptozotocin-induced diabetic hamsters

Abstract

OBJECTIVES:Normal endothelial cells respond to shear stress by elongating and aligning in the direction of fluid flow. Hyperglycemia impairs this response and contributes to microvascular complications, which result in deleterious effects to the endothelium. This work aimed to evaluate cheek pouch microvessel morphological characteristics, reactivity, permeability, and expression of cytoskeleton and extracellular matrix components in hamsters after the induction of diabetes with streptozotocin.METHODS:Syrian golden hamsters (90–130 g) were injected with streptozotocin (50 mg/kg, i.p.) or vehicle either 6 (the diabetes mellitus 6 group) or 15 (the diabetes mellitus 15 group) days before the experiment. Vascular dimensions and density per area of vessels were determined by morphometric and stereological measurements. Changes in blood flow were measured in response to acetylcholine, and plasma extravasation was measured by the number of leakage sites. Actin, talin, α-smooth muscle actin, vimentin, type IV collagen, and laminin were detected by immunohistochemistry and assessed through a semiquantitative scoring system.RESULTS:There were no major alterations in the lumen, wall diameters, or densities of the examined vessels. Likewise, vascular reactivity and permeability were not altered by diabetes. The arterioles demonstrated increased immunoreactivity to vimentin and laminin in the diabetes mellitus 6 and diabetes mellitus 15 groups.DISCUSSION:Antibodies against laminin and vimentin inhibit branching morphogenesis in vitro. Therefore, laminin and vimentin participating in the structure of the focal adhesion may play a role in angiogenesis.CONCLUSIONS:Our results indicated the existence of changes related to cell–matrix interactions, which may contribute to the pathological remodeling that was already underway one week after induction of experimental diabetes.

Co-occurrence of opisthorchiasis and diabetes exacerbates morbidity of the hepatobiliary tract disease

“…uninfected and without diabetes. Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg body weight of streptozotocin (STZ) dissolved in 0.1 M tri-sodium citrate buffer, pH 4.5 (Sigma Chemical Co., St. Louis, MO) each day for three consecutive days [ 64 ]. Hamsters in the non-diabetes groups, i.e.…”

Cardiovascular research in CLINICS

“…Silva et al (29) aimed to evaluate cheek-pouch microvessel morphological characteristics, reactivity, permeability, and expression of cytoskeletal and extracellular matrix components in hamsters with streptozotocin-induced diabetes. The authors reported precocious changes that were related to cell-matrix interactions and may contribute to the pathological remodeling that was already underway one week after the induction of experimental diabetes.…”