Vigna angularis inhibits IL-6-induced cellular signalling and ameliorates collagen-induced arthritis

Oh, Hyun-Mee; Lee, Seung Woong; Yun, Bo Ra; Hwang, Byung Kook; Kim, So Nam; Park, Chan Sun; Jeoung, See-Hwa; Kim, Hyun-Kyu; Lee, Woo Song; Rho, Mun‐Chual

doi:10.1093/rheumatology/ket302

Cited by 27 publications

(24 citation statements)

References 32 publications

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“…Hep3B cells stably expressing pSTAT3-Luc were established as described previously (Oh et al 2014). The Hep3B cells stably expressing pSTAT3-Luc were seeded onto 96-well culture plates at 2 9 10 4 cells/well.…”

Section: Il-6-induced Stat3 Activationmentioning

confidence: 99%

Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation

et al. 2017

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This study constitutes the first attempt to isolate various LABs from Gajuknamu kimchi and to discover IL-6/STAT3 inhibitors in the EtOAc extract of Lc. lactis KR-050L culture broth. Moreover, our data provide useful biochemical information regarding the commercialization of Lc. lactis isolated from Gajuknamu kimchi as an approach to use functional foods for the treatment of various diseases via IL-6/STAT3 activation.

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Section: Il-6-induced Stat3 Activationmentioning

confidence: 99%

Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation

et al. 2017

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“…IL-6 is a member of the neuropoietin family of cytokines which includes leukemia inhibitory factor, ciliary neurotrophic factor, cardiotrophin oncostatin M, and IL-11 [16,17]. And activated IL-6 receptor recruits JAK1, JAK2, and Tyk2 tyrosine kinases which can phosphorylate STAT1 and STAT3 [18,19]. Midazolam is a potent benzodiazepine derivative with powerful hypnotic, sedative, anxiolytic, amnestic, anticonvulsant and muscle-relaxant properties by modulating the GABA A receptor in the central nervous system [20] .…”

Section: Introductionmentioning

confidence: 99%

Midazolam Inhibits the Apoptosis of Astrocytes Induced by Oxygen Glucose Deprivation via Targeting JAK2-STAT3 Signaling Pathway

Liu

You²,

Tu³

et al. 2015

Cell Physiol Biochem

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Background: There is an increasing interest in the role of astrocytes contributing to the intrinsic bioremediation of ischemic brain injury. The purpose of this study was to disclose the effects and mechanism of midazolam (MDZ) on the proliferation and apoptosis of astrocytes under oxygen glucose deprivation (OGD) condition. Methods: The astrocytes were assigned randomly into four groups: control group, OGD group, OGD+MDZ group, and OGD+MDZ+IL-6 group. The astrocytes were treated with MDZ at dose of 10 μmol/L in OGD+MDZ group. And in OGD+MDZ+IL-6 group, the astrocytes were treated with MDZ at dose of 10μmol/L and IL-6 at dose of 50 ng/mL. MTT assay was used to assess cell proliferation, and cell apoptosis was analyzed by TUNEL apoptosis assay kit and flow cytometry. Furthermore, the expression of JAK2, p-JAK2, STAT3, p-STAT3, Bcl-2, Bax and Caspase-3 proteins were determined by western blotting assay. Results: Astrocytes proliferation was decreased obviously in OGD group, while MDZ could increase astrocytes proliferation under OGD condition. Moreover, OGD could induce apoptosis in astrocytes and MDZ could play an anti-apoptotic role. However, IL-6, a JAK2 activator, could attenuate cell proliferation and anti-apoptotic effects of MDZ in astrocytes. In addition, the expression of Bcl-2 protein in MDZ group increased markedly, while the JAK2/STAT3 signal proteins, Bax and Caspase-3 proteins decreased relative to OGD group. But IL-6 could counteract the anti-apoptotic effects of MDZ. Conclusion: Midazolam has protective effects on the proliferation and apoptosis of astrocytes via JAK2/STAT3 signal pathway in vitro. We firstly disclose the beneficial roles of midazolam in astrocytes under ischemic condition, which may be a rational treatment selection for ischemic cerebral protection.

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“…The JAK2/STAT3 cascade is responsible for IL-6-mediated cellular responses in both physiological and pathological events. The interference with STAT3 signalling could be a good therapeutic strategy to mitigate autoimmune diseases, including RA [60]. In our study, CTX reduced the IL-1 β -induced elevation of IL-6 and p-STAT3 protein levels in FLS.…”

Section: Discussionmentioning

confidence: 53%

Suppression of Inflammation and Arthritis by Orally Administrated Cardiotoxin fromNaja naja atra

Chen

Jie-yu

Kou

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Cardiotoxin (CTX) from Naja naja atra venom (NNAV) reportedly had analgesic effect in animal models but its role in inflammation and arthritis was unknown. In this study, we investigated the analgesic, anti-inflammatory, and antiarthritic actions of orally administered CTX-IV isolated from NNAV on rodent models of inflammation and adjuvant arthritis. CTX had significant anti-inflammatory effects in models of egg white induced nonspecific inflammation, filter paper induced rat granuloma formation, and capillary osmosis tests. CTX significantly reduced the swelling of paw induced by egg white, the inflammatory exudation, and the formation of granulomas. CTX reduced the swelling of paw, the AA clinical scores, and pathological alterations of joint. CTX significantly decreased the number of the CD4 T cells and inhibited the expression of relevant proinflammatory cytokines IL-17 and IL-6. CTX significantly inhibited the secretion of proinflammatory cytokine IL-6 and reduced the level of p-STAT3 in FLS. These results suggest that CTX inhibits inflammation and inflammatory pain and adjuvant-induced arthritis. CTX may be a novel therapeutic drug for treatment of arthritis.

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Vigna angularis inhibits IL-6-induced cellular signalling and ameliorates collagen-induced arthritis

Abstract: Administration of VAE has a therapeutic effect on CIA and this effect is associated with the inhibitory activity on IL-6/STAT3 signalling. These results suggest that extracts or compounds from V. angularis could be a useful treatment for diseases related to IL-6, including RA.

Cited by 27 publications

References 32 publications

Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation

Lactococcus lactis KR-050L inhibit IL-6/STAT3 activation

Midazolam Inhibits the Apoptosis of Astrocytes Induced by Oxygen Glucose Deprivation via Targeting JAK2-STAT3 Signaling Pathway

Suppression of Inflammation and Arthritis by Orally Administrated Cardiotoxin fromNaja naja atra

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