Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

Lei, Ming; Winterhalder, Martin; Selm, Romedi; Zumbusch, Andreas

doi:10.1117/1.3533707

Cited by 23 publications

(19 citation statements)

References 18 publications

(36 reference statements)

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“…CARS microscopy constitutes a fast imaging technique: The image acquisition rate ranges from few Hz in our experimental setup to more than 20 Hz. [37], [38] Therefore, the acquisition speed is compatible with in vivo applications. Further technical developments will pave the way to a clinical, intraoperative application.…”

Section: Resultsmentioning

confidence: 99%

Label-Free Delineation of Brain Tumors by Coherent Anti-Stokes Raman Scattering Microscopy in an Orthotopic Mouse Model and Human Glioblastoma

et al. 2014

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BackgroundCoherent anti-Stokes Raman scattering (CARS) microscopy provides fine resolution imaging and displays morphochemical properties of unstained tissue. Here, we evaluated this technique to delineate and identify brain tumors.MethodsDifferent human tumors (glioblastoma, brain metastases of melanoma and breast cancer) were induced in an orthotopic mouse model. Cryosections were investigated by CARS imaging tuned to probe C-H molecular vibrations, thereby addressing the lipid content of the sample. Raman microspectroscopy was used as reference. Histopathology provided information about the tumor's localization, cell proliferation and vascularization.ResultsThe morphochemical contrast of CARS images enabled identifying brain tumors irrespective of the tumor type and properties: All tumors were characterized by a lower CARS signal intensity than the normal parenchyma. On this basis, tumor borders and infiltrations could be identified with cellular resolution. Quantitative analysis revealed that the tumor-related reduction of CARS signal intensity was more pronounced in glioblastoma than in metastases. Raman spectroscopy enabled relating the CARS intensity variation to the decline of total lipid content in the tumors. The analysis of the immunohistochemical stainings revealed no correlation between tumor-induced cytological changes and the extent of CARS signal intensity reductions. The results were confirmed on samples of human glioblastoma.ConclusionsCARS imaging enables label-free, rapid and objective identification of primary and secondary brain tumors. Therefore, it is a potential tool for diagnostic neuropathology as well as for intraoperative tumor delineation.

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Section: Resultsmentioning

confidence: 99%

Label-Free Delineation of Brain Tumors by Coherent Anti-Stokes Raman Scattering Microscopy in an Orthotopic Mouse Model and Human Glioblastoma

et al. 2014

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“…The second important difference concerns the image integration times. Compared to spontaneous Raman microscopy, non-linear Raman imaging is several orders of magnitudes faster, with video-rate imaging being achievable [25][26][27]. However, the fast imaging capability comes at the expense of significantly reduced spectral information of one image, because most non-linear Raman microscopy implementations probe only one vibrational resonance at a time.…”

Section: Technical Backgroundmentioning

confidence: 98%

Beyond the borders — Biomedical applications of non-linear Raman microscopy

Winterhalder

Zumbusch

2015

Advanced Drug Delivery Reviews

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“…Narrow-band CRI methods have demonstrated image acquisition at video rate 20,21 but these derive contrast from single Raman bands, typically from the CH stretch region (2800 to 3200 cm −1 ). These narrow-band coherent Raman methods are limited in their utility for obtaining the types of functional and structural information referred to above, which comes from multiple peaks in the fingerprint region.…”

Section: Bodymentioning

confidence: 99%

Molecular imaging with CARS micro-spectroscopy

Cicerone¹

2016

Current Opinion in Chemical Biology

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After more than a decade of instrument and method development, broadband coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy is beginning to live up to its potential as a label-free imaging modality that can rapidly generate high resolution images with full vibrational spectra at each image pixel. Presently these instruments are able to obtain quantitative, spatially resolved information on lipids from the CH stretch region of the Raman spectrum, and some instrument designs facilitate acquisition of high quality fingerprint spectra, containing information on a host of molecular species including structural proteins, nucleotides, and metabolites. While most of the existing instruments are research projects themselves, it appears that the relevant technologies are maturing so that commercially available instruments may not be too far in the future, making this remarkable imaging modality widely available.

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Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

Cited by 23 publications

References 18 publications

Label-Free Delineation of Brain Tumors by Coherent Anti-Stokes Raman Scattering Microscopy in an Orthotopic Mouse Model and Human Glioblastoma

Label-Free Delineation of Brain Tumors by Coherent Anti-Stokes Raman Scattering Microscopy in an Orthotopic Mouse Model and Human Glioblastoma

Beyond the borders — Biomedical applications of non-linear Raman microscopy

Molecular imaging with CARS micro-spectroscopy

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