Beyond the borders — Biomedical applications of non-linear Raman microscopy

Winterhalder, Martin; Zumbusch, Andreas

doi:10.1016/j.addr.2015.04.024

Cited by 21 publications

(18 citation statements)

References 150 publications

(167 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One major limitation is the long acquisition time required to construct the Raman image. Several approaches can be used to overcome this limitation providing a faster imaging modality and allowing investigation of several sperm cells simultaneously, these include (i) the use of galvanomirrors to scan the beam and capture a two-dimensional data set containing all spectra of one spatial line [36], (ii) the use of spatial light modulators and orthogonal illuminating fields to realize a scan-free spontaneous Raman imaging system [53], (iii) the use of a faster Raman-based imaging approach, such as CARS microscopy [79][80][81][82][83][84] or SERS microscopy [29,[85][86][87][88][89][90], providing chemical images with large fields of view at a video rate.…”

Section: Discussionmentioning

confidence: 99%

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Angelis

Managò

Ferrara

et al. 2017

Journal of Spectroscopy

View full text Add to dashboard Cite

The diagnosis of male infertility is vastly complex. To date, morphology, motility, and concentration have been used as key parameters to establish the sperm normality and achieve pregnancy both in natural and in assisted fecundation. However, spermatozoa from infertile men could present a variety of alterations, such as DNA fragmentation, alterations of chromatin structure, and aneuploidy, which have been demonstrated to decrease reproductive capacity of men. Therefore, the ability to see detailed relationships between morphology and physiology in selected spermatozoa with submicrometric resolution in a nondestructive and noninvasive way and within a functional correlated context could be extremely important for the intracytoplasmic sperm injection procedure. In this review, we describe label-free optical spectroscopy and imaging techniques, based on the combination of Raman spectroscopy/imaging with holographic imaging, which are able to noninvasively measure the (bio)chemistry and morphology of sperm cells. We discuss the benefits and limitation of the proposed photonic techniques, with particular emphasis on applications in detection/characterization of sperm cell morphological defects and photodamage, and the identification/sorting of X- and Y-bearing bovine spermatozoa.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Angelis

Managò

Ferrara

et al. 2017

Journal of Spectroscopy

View full text Add to dashboard Cite

show abstract

“…These techniques can allow relatively high‐resolution visualization of the epidermis and give insight into chemical composition . However, their rate of image capture can be slow, and in some cases, sensitivity when imaging the skin may be low . Reflectance confocal microscopy (RCM) provides excellent lateral resolution with which to visualize the skin down to the papillary dermis in vivo .…”

mentioning

confidence: 99%

Novel approaches to characterize age‐related remodelling of the dermal‐epidermal junction in 2D, 3D and in vivo

Newton

Bradley

Séroul³

et al. 2016

Skin Research and Technology

View full text Add to dashboard Cite

Background/purpose: The dermal-epidermal junction (DEJ) forms epidermal protrusions down into the dermis (rete ridges) and dermal projections up into the epidermis (dermal papillae). Usually visualized in two-dimensions (2D), our knowledge of how the DEJ changes with ageing is limited. We aimed to characterize how this structure exists in 3D and changes with age. Methods: Photoprotected and photoexposed skin were imaged using reflectance confocal microscopy (RCM) in young and aged individuals. Biopsies of the imaged areas were processed for histological sectioning and for imaging using microcomputed X-ray tomography (microCT). Results: Images obtained from RCM and microCT were used to 3D reconstruct the DEJ. DEJ heights obtained from microCT images showed strong correlation with histology-measured heights. We proposed a novel definition of rete ridges (RR m ) and dermal papillae (DP m ), which allowed easier automated measurement of reduced DP m and RR m volumes in aged skin from microCT reconstructions. An algorithm to map DP m connectivity showed reduced lengths of DP m branches with age.

show abstract

“…As a result, spectral integration times can be reduced compared to conventional Raman spectroscopy to tens of ms, rendering CARS a valuable novel tool for in situ quantitative monitoring of dynamic molecular processes on the nanoscale. Commonly, CARS is employed in the field of bio(medical) imaging and has only recently been introduced to the field of materials science. In the following, we highlight the potential CARS holds for the study of important (electro)chemical processes occurring inside nanopores, such as the catalytic chemical conversion on zeolite particles and the interaction and diffusion of water inside fuel‐cell membranes.…”

Section: Monitoring Molecular Interactions and Diffusion In Nanoporesmentioning

confidence: 99%

Raman Under Water – Nonlinear and Nearfield Approaches for Electrochemical Surface Science

Sabanés

Domke

2017

ChemElectroChem

View full text Add to dashboard Cite

Electrochemistry is re‐gaining attention among scientists because the complex interplay between electronic and chemical interfacial processes lies at the bottom of a broad range of important research disciplines like alternative energy conversion or green catalysis and synthesis. While rapid progress has been made in recent years regarding novel technological applications, the community increasingly recognizes that the understanding of the molecular processes that govern macroscopic device properties is still rather limited – which hinders a systematic and more complete exploration of novel material and functionality space. Here, we discuss advanced Raman spectroscopies as valuable analysis tools for electrochemists. The chemical nature of a material and its interaction with the environment is contained in the label‐free vibrational fingerprint over a broad energy range so that organic species, solid‐state materials, and hybrids thereof can be investigated alike. For surface studies, the inherently small Raman scattering cross sections can be overcome with advanced nonlinear or nearfield‐based approaches that provide signal enhancements between three and seven orders of magnitude, sufficient to detect few scatterers in nano‐confined spaces or adsorbate (sub)monolayers. Our article highlights how advanced Raman techniques with extreme chemical, spatial and temporal resolution constitute valuable alternative surface analysis tools and provide otherwise inaccessible information about complex interfacial (electro)chemical processes.

show abstract

Beyond the borders — Biomedical applications of non-linear Raman microscopy

Cited by 21 publications

References 150 publications

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Novel approaches to characterize age‐related remodelling of the dermal‐epidermal junction in 2D, 3D and in vivo

Raman Under Water – Nonlinear and Nearfield Approaches for Electrochemical Surface Science

Contact Info

Product

Resources

About